pCPC155 vector (V008265)

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LOCUS       V008265                 6381 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008265
VERSION     V008265
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6381)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6381)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6381)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6381)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-JAN-2018) Research Center of Bioenergy "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6381
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(693..797)
                     /label="AmpR promoter"
     CDS             1766..2884
                     /gene="LEU2"
                     /label="3-isopropylmalate dehydrogenase"
                     /note="3-isopropylmalate dehydrogenase from Candida
                     maltosa. Accession#: P07139"
     protein_bind    complement(3137..3170)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4729..4755
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             4759..4785
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             4789..4815
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     rep_origin      complement(5454..6042)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(join(6216..6381,1..692))
                     /label="AmpR"
                     /note="beta-lactamase"