LOCUS       V008265                 6381 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008265
VERSION     V008265
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6381)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6381)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6381)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6381)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-JAN-2018) Research Center of Bioenergy "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6381
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(693..797)
                     /label="AmpR promoter"
     CDS             1766..2884
                     /gene="LEU2"
                     /label="3-isopropylmalate dehydrogenase"
                     /note="3-isopropylmalate dehydrogenase from Candida
                     maltosa. Accession#: P07139"
     protein_bind    complement(3137..3170)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4729..4755
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             4759..4785
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             4789..4815
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     rep_origin      complement(5454..6042)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(join(6216..6381,1..692))
                     /label="AmpR"
                     /note="beta-lactamase"
ORIGIN
        1 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
       61 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
      121 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
      181 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
      241 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
      301 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
      361 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
      421 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
      481 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
      541 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
      601 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
      661 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
      721 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
      781 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaataac
      841 ttcgtatagc atacattata cgaagttata gctcggatcc actagtaacg gccgccagtg
      901 tgctggaatt cgcccttgga tccaatcatc actggtgggg gtcaagaaag aggtttgaga
      961 tcaggtactt tggcaccacc attagtagct gggtttggtg aagctgcaag attaatgaaa
     1021 caagaatctg catttgacaa gaaacatatt gagaggttat ctactaaatt gaagaacggt
     1081 ttgttatcca ttccatctac tcagtttaac ggttgtaata acccaacata tcaataccca
     1141 ggttgtgtga atgtttcctt tgcatacatt gaaggggaat ctttgttaat ggcattgaaa
     1201 gatattgcat tgagttctgg ttctgcatgt acctctgcat ctttggaacc atcgtatgtt
     1261 ttacatgctt tgggtgccga tgatgccttg gctcattctt ccattagatt cggtattggt
     1321 agattcacca ctgaagcaga agttgactat gttattcaag caattaatga aagagttgat
     1381 ttcttgagaa agatgtctcc attatgggaa atggttcaag aaggtattga cttgaactct
     1441 atcgaatgga gtggacatta aggttttttg tttttttgta atttgtgttg ttatgtgtaa
     1501 ttatcgtgta tagagtaatt tcggaaattg tactttcgtt catgtatctg aaccagatga
     1561 ataatacgtt cgtattgttg aagaatgact aactcctgta acggcgtgcg tgggttccgc
     1621 agccgggttc aatttttttt ttcttgttca ctataaaaat atataataaa cgaaaaaaat
     1681 ccgagggact caggctatga aatttttcac tcaagaattt tttttccttc atccttaatt
     1741 cctttattca ctacccaaac ccacaatgtc agttaaaact aaaactatta caattttacc
     1801 aggtgatcac gtcggtaccg aaattgtcaa tgaagctatc aaagttttag aagcaattga
     1861 agctgccact ccttaccaaa aaatccattt cgatttcaaa catcatttaa tcggtggtgc
     1921 tgccattgat gccactggtg tcccattgcc agatgacgca cttgaaagtg ccaaaaaatc
     1981 tgatgccgtc ttattgggtg ctgttggtgg accaaaatgg gggactggcg ccgttcgtcc
     2041 agaacaaggt ttattaaaga ttcgtaaaga attgaacctt tatgctaaca tcagaccatg
     2101 taactttgcc agtgactcct tattggaatt atccccattg agaccagaag tcgttaaagg
     2161 tacaaacttg atcattgttc gtgaattagt tggtggtatt tatttcggtg atcgtgaaga
     2221 acaagaagaa agtgaagata aacaaaccgc atgggatact gaaaaataca ccgtcgatga
     2281 agtcaccaga atcacccgta tggctgcctt catggcttta caacataacc caccattacc
     2341 aatctggtca ttggataagg ctaatgtttt agcttcttct agattatgga gaagaactgt
     2401 cgataaagtg atttctgaag agttcccaac tttgtctgtt caacatcaat tgattgactc
     2461 cgccgccatg attttaattc aaaacccaac caaattgaat ggtataatca tcacttctaa
     2521 catgtttggt gatatcattt ccgatgaagc ttcagttatt ccaggttcct tgggtttatt
     2581 accatctgca tctttggctt cattgccaga taccaacacc gcttttggtc tttatgaacc
     2641 atgtcacggt tctgctcctg atttaccagc caataaagtt aacccaattg ctactatttt
     2701 atctgctgct tctatgttga gattatcttt ggattgtgtg aaagaagctg aagctttaga
     2761 agaagccgtg aaacaagtct tggataaagg tatcagaact gcagatttaa gaggtaccag
     2821 ttcagctact gaagttggtg acgcgattgt cgaagctgtt actaaaatct taaaagaaaa
     2881 agcttaagta aagtatacac aaaatttaac gaatattatt aatacaaaat gaacaccacc
     2941 taaatacttt tttttaaaaa tgtaccgact cttctaaaaa ttgcgataat tcaatatcgg
     3001 aaatttctct caccggttta cttggatctt cggaaataaa tctatttgaa gtaatggtag
     3061 cctcacttga ttctgattgg ctctttctag acatgggtag gtaagggcga attctgcaga
     3121 tatccatcac actggtataa cttcgtatag catacattat acgaagttat ggtaatacgg
     3181 ttatccacag aatcgatccg tctaggattg aacgatgata ttaagcgaga attcacattt
     3241 ttctagagga atcacagtta caattgtaga ggaaaccgaa ctagtaaaac tagcacccaa
     3301 cacttctaaa caaaccaatc aattcaatgg tataacaaat cgtctaccag aagttatgct
     3361 aatatgaaac atcaattgac aacatggaca tcaactgaag ctttaacaaa tgaataaaaa
     3421 aaaaagccca tacaatagac cattggtcat agtttcattt gcccccgccc cacttttatt
     3481 gttgagttga ataattgtga gcatcagctg attttaaaat tataaatttt ttttttcgtc
     3541 cattgttaat tctattttag ttttttcttc tccatcgtct ccgaattatt ccggaagctg
     3601 gtagcggata agttttagaa tcattgagcc tcttttcaat gccagatgtt atctgcaact
     3661 gatggttagt tagttagtat aagacttaaa ttctcatata ggtcatgtgc acggacaagc
     3721 tcattgagtg acgaaaagtc aaatttaaac ttctctaaac aaatttcaga caatagtccg
     3781 aaaaaaaaaa acaaaaccca ttattgtcga catgtcaaag gattcaacgg ttgagatgga
     3841 gccgtcaaat atccaaaaca ctcttctttc aactaatttg ccgtaaacta tctccatcgt
     3901 acgattaaga taaaaaaaaa caattatttc ttacaatatt tgatagagac ccaatgcaaa
     3961 gccagcgaga aagaggagta ttggcattgt tgggtagtag atgaatgagt aatcttctag
     4021 ccaaaacaaa ataaaccaca agaacagtag gcaaaactga gtacttgata gcgcttgtag
     4081 tctttgtatt taaaaaaacc taattgtcca aaaattgaaa atcatgagtt gcaaatttgt
     4141 tgaaatcacc aggtaaatag atcatttagc tatatcgcct tatcattctt cgtacaagaa
     4201 ttatacacaa ataccaccac cacaccaccc agaaatttta cacgtgtttt ctaaattatt
     4261 actttccgtg gccaatcaaa ttgaaatttt tcttgttcga aacggcagtg cctcaaacat
     4321 tatagtagca agtattgcct aaagagtttc cgtctcgtgc ccccccccta tccccttttg
     4381 taatttgcca aaatagctca aaaaggataa tcccgcactt tttttttccg agttctcgtc
     4441 atgcaagcaa gcttattcag aattttcaga ggtgcgagtc tcaccactac cactgcagct
     4501 gcatctttta cagcaacagc aacagcaggt gccaccacgg caaagacatt gtctggatcc
     4561 actgtgctcc gaaaactgta taaaaggacc tattcatccc tggtcttatc ttctccagaa
     4621 ttattttttt ttcatcagtt taacaacaac aaacgttatt gtcatacaac aacaacaaca
     4681 ttattcatgt ccacacgcaa gctcgcgagc gcgccaatta gatccgtgta cccatacgat
     4741 gttccagatt acgctggtta cccatacgat gttccagatt acgctggtta cccatacgat
     4801 gttccagatt acgctatcga tatcccatgg agatctacgc taggatcgat atctcgagac
     4861 gcgtgatctt ggacaccagt aaataagcaa atagctaaat catatacgaa ttaatgttat
     4921 gattaagtgt ttacgtgagt gcgatatttt tattactatc ttatacagtt gtatatactc
     4981 tataaaatga gttgtctatt aattaacgat gaatgctttc tgggtttacc tctccaacga
     5041 ctctagttta cttctcaata cattcaattg tatttgattt gtcaatactt catcattaat
     5101 caattctata gttttgtttt tctcgtttat ttccaaattt aatgcatcaa ttttattatt
     5161 caatttgtcg ttgattttgg ttaatgattt tatggtttga tctctggcat tgattgtttg
     5221 tgttagtttt tcattattga taattaaact atttaagtta gttatcaact cggtgttttc
     5281 aagttttaag ttttcaattt ctttagagtt tataagattt gtcaaagttt ctgaattgct
     5341 tgattggtcc tgtagaagcg tgtctaagaa ctcgaggata acgcaggaaa gaacatgtga
     5401 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     5461 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     5521 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     5581 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     5641 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     5701 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     5761 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     5821 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     5881 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     5941 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     6001 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     6061 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     6121 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     6181 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     6241 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     6301 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     6361 cgctcaccgg ctccagattt a
//
