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pCPC152 vector (V008268)

Basic Vector Information

Vector Name:
pCPC152
Antibiotic Resistance:
Ampicillin
Length:
6277 bp
Type:
Expression vector
Replication origin:
ori
Source/Author:
Chang P, Wang W, Chen J.

pCPC152 vector Map

Copy Sequence View Map
pCPC1526277 bp30060090012001500180021002400270030003300360039004200450048005100540057006000AmpRAmpR promoter3-isopropylmalate dehydrogenaseloxPMycori

pCPC152 vector Sequence

LOCUS       V008268                 6277 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008268
VERSION     V008268
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6277)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6277)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6277)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6277)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-JAN-2018) Research Center of Bioenergy "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6277
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(255..1112)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1113..1217)
                     /label="AmpR promoter"
     CDS             2186..3304
                     /gene="LEU2"
                     /label="3-isopropylmalate dehydrogenase"
                     /note="3-isopropylmalate dehydrogenase from Candida
                     maltosa. Accession#: P07139"
     protein_bind    complement(3557..3590)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             5176..5205
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     rep_origin      complement(join(5770..6277,1..81))
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"

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