LOCUS       V008268                 6277 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008268
VERSION     V008268
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6277)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6277)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6277)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6277)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-JAN-2018) Research Center of Bioenergy "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6277
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(255..1112)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1113..1217)
                     /label="AmpR promoter"
     CDS             2186..3304
                     /gene="LEU2"
                     /label="3-isopropylmalate dehydrogenase"
                     /note="3-isopropylmalate dehydrogenase from Candida
                     maltosa. Accession#: P07139"
     protein_bind    complement(3557..3590)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             5176..5205
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     rep_origin      complement(join(5770..6277,1..81))
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
       61 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
      121 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
      181 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
      241 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
      301 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
      361 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
      421 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
      481 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
      541 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
      601 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
      661 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
      721 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
      781 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
      841 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
      901 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
      961 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     1021 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     1081 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     1141 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     1201 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaataac
     1261 ttcgtatagc atacattata cgaagttata gctcggatcc actagtaacg gccgccagtg
     1321 tgctggaatt cgcccttgga tccaatcatc actggtgggg gtcaagaaag aggtttgaga
     1381 tcaggtactt tggcaccacc attagtagct gggtttggtg aagctgcaag attaatgaaa
     1441 caagaatctg catttgacaa gaaacatatt gagaggttat ctactaaatt gaagaacggt
     1501 ttgttatcca ttccatctac tcagtttaac ggttgtaata acccaacata tcaataccca
     1561 ggttgtgtga atgtttcctt tgcatacatt gaaggggaat ctttgttaat ggcattgaaa
     1621 gatattgcat tgagttctgg ttctgcatgt acctctgcat ctttggaacc atcgtatgtt
     1681 ttacatgctt tgggtgccga tgatgccttg gctcattctt ccattagatt cggtattggt
     1741 agattcacca ctgaagcaga agttgactat gttattcaag caattaatga aagagttgat
     1801 ttcttgagaa agatgtctcc attatgggaa atggttcaag aaggtattga cttgaactct
     1861 atcgaatgga gtggacatta aggttttttg tttttttgta atttgtgttg ttatgtgtaa
     1921 ttatcgtgta tagagtaatt tcggaaattg tactttcgtt catgtatctg aaccagatga
     1981 ataatacgtt cgtattgttg aagaatgact aactcctgta acggcgtgcg tgggttccgc
     2041 agccgggttc aatttttttt ttcttgttca ctataaaaat atataataaa cgaaaaaaat
     2101 ccgagggact caggctatga aatttttcac tcaagaattt tttttccttc atccttaatt
     2161 cctttattca ctacccaaac ccacaatgtc agttaaaact aaaactatta caattttacc
     2221 aggtgatcac gtcggtaccg aaattgtcaa tgaagctatc aaagttttag aagcaattga
     2281 agctgccact ccttaccaaa aaatccattt cgatttcaaa catcatttaa tcggtggtgc
     2341 tgccattgat gccactggtg tcccattgcc agatgacgca cttgaaagtg ccaaaaaatc
     2401 tgatgccgtc ttattgggtg ctgttggtgg accaaaatgg gggactggcg ccgttcgtcc
     2461 agaacaaggt ttattaaaga ttcgtaaaga attgaacctt tatgctaaca tcagaccatg
     2521 taactttgcc agtgactcct tattggaatt atccccattg agaccagaag tcgttaaagg
     2581 tacaaacttg atcattgttc gtgaattagt tggtggtatt tatttcggtg atcgtgaaga
     2641 acaagaagaa agtgaagata aacaaaccgc atgggatact gaaaaataca ccgtcgatga
     2701 agtcaccaga atcacccgta tggctgcctt catggcttta caacataacc caccattacc
     2761 aatctggtca ttggataagg ctaatgtttt agcttcttct agattatgga gaagaactgt
     2821 cgataaagtg atttctgaag agttcccaac tttgtctgtt caacatcaat tgattgactc
     2881 cgccgccatg attttaattc aaaacccaac caaattgaat ggtataatca tcacttctaa
     2941 catgtttggt gatatcattt ccgatgaagc ttcagttatt ccaggttcct tgggtttatt
     3001 accatctgca tctttggctt cattgccaga taccaacacc gcttttggtc tttatgaacc
     3061 atgtcacggt tctgctcctg atttaccagc caataaagtt aacccaattg ctactatttt
     3121 atctgctgct tctatgttga gattatcttt ggattgtgtg aaagaagctg aagctttaga
     3181 agaagccgtg aaacaagtct tggataaagg tatcagaact gcagatttaa gaggtaccag
     3241 ttcagctact gaagttggtg acgcgattgt cgaagctgtt actaaaatct taaaagaaaa
     3301 agcttaagta aagtatacac aaaatttaac gaatattatt aatacaaaat gaacaccacc
     3361 taaatacttt tttttaaaaa tgtaccgact cttctaaaaa ttgcgataat tcaatatcgg
     3421 aaatttctct caccggttta cttggatctt cggaaataaa tctatttgaa gtaatggtag
     3481 cctcacttga ttctgattgg ctctttctag acatgggtag gtaagggcga attctgcaga
     3541 tatccatcac actggtataa cttcgtatag catacattat acgaagttat ggtaatacgg
     3601 ttatccacag aatcgatccg tctaggattg aacgatgata ttaagcgaga attcacattt
     3661 ttctagagga atcacagtta caattgtaga ggaaaccgaa ctagtaaaac tagcacccaa
     3721 cacttctaaa caaaccaatc aattcaatgg tataacaaat cgtctaccag aagttatgct
     3781 aatatgaaac atcaattgac aacatggaca tcaactgaag ctttaacaaa tgaataaaaa
     3841 aaaaagccca tacaatagac cattggtcat agtttcattt gcccccgccc cacttttatt
     3901 gttgagttga ataattgtga gcatcagctg attttaaaat tataaatttt ttttttcgtc
     3961 cattgttaat tctattttag ttttttcttc tccatcgtct ccgaattatt ccggaagctg
     4021 gtagcggata agttttagaa tcattgagcc tcttttcaat gccagatgtt atctgcaact
     4081 gatggttagt tagttagtat aagacttaaa ttctcatata ggtcatgtgc acggacaagc
     4141 tcattgagtg acgaaaagtc aaatttaaac ttctctaaac aaatttcaga caatagtccg
     4201 aaaaaaaaaa acaaaaccca ttattgtcga catgtcaaag gattcaacgg ttgagatgga
     4261 gccgtcaaat atccaaaaca ctcttctttc aactaatttg ccgtaaacta tctccatcgt
     4321 acgattaaga taaaaaaaaa caattatttc ttacaatatt tgatagagac ccaatgcaaa
     4381 gccagcgaga aagaggagta ttggcattgt tgggtagtag atgaatgagt aatcttctag
     4441 ccaaaacaaa ataaaccaca agaacagtag gcaaaactga gtacttgata gcgcttgtag
     4501 tctttgtatt taaaaaaacc taattgtcca aaaattgaaa atcatgagtt gcaaatttgt
     4561 tgaaatcacc aggtaaatag atcatttagc tatatcgcct tatcattctt cgtacaagaa
     4621 ttatacacaa ataccaccac cacaccaccc agaaatttta cacgtgtttt ctaaattatt
     4681 actttccgtg gccaatcaaa ttgaaatttt tcttgttcga aacggcagtg cctcaaacat
     4741 tatagtagca agtattgcct aaagagtttc cgtctcgtgc ccccccccta tccccttttg
     4801 taatttgcca aaatagctca aaaaggataa tcccgcactt tttttttccg agttctcgtc
     4861 atgcaagcaa gcttattcag aattttcaga ggtgcgagtc tcaccactac cactgcagct
     4921 gcatctttta cagcaacagc aacagcaggt gccaccacgg caaagacatt gtctggatcc
     4981 actgtgctcc gaaaactgta taaaaggacc tattcatccc tggtcttatc ttctccagaa
     5041 ttattttttt ttcatcagtt taacaacaac aaacgttatt gtcatacaac aacaacaaca
     5101 ttattcatgt ccacacccgt tcccgggtta attaacggtg aacaaaagct aatctccgag
     5161 gaatacttga acggtgaaca aaaattaatc tcagaagaag acttgaacgg actcgacggt
     5221 gaacaaaagt tgatttctga agaagatttg aacggtgaac aaaagctaat ctccgaggaa
     5281 gacttgaacg gtgaacaaaa gttaatctca gaagaagact tgaacggact cgacggtgaa
     5341 caaaagttga tttctgaaga agatttgaac ggtgaacaaa agctaatctc cgaggaagac
     5401 ttgaacggtg aacaaaaatt aatctcagaa gaagacttga acggactcga cggtgaacaa
     5461 aagttgattt ctgaagaaga tttgaacggt gaacaaaagc taatctccga ggaagacttg
     5521 aacggtgaac aaaaattaat ctcagaagaa gacttgaacg gactcgacgg tgaacaaaag
     5581 ttgatttctg aagaagattt gaacggtgaa caaaagctaa tctccgagga agacttgaac
     5641 ggtgaacaaa aattaatcaa tcacgcatgc gattatagtc taagaactcg aggataacgc
     5701 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     5761 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     5821 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     5881 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     5941 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     6001 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     6061 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     6121 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     6181 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     6241 gccagttacc ttcggaaaaa gagttggtag ctcttga
//
