Basic Vector Information
- Vector Name:
- pCPC120
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6336 bp
- Type:
- Expression vector
- Replication origin:
- ori
- Source/Author:
- Chang P, Wang W, Chen J.
pCPC120 vector Map
pCPC120 vector Sequence
LOCUS V008269 6336 bp DNA circular SYN 17-DEC-2018 DEFINITION Exported. ACCESSION V008269 VERSION V008269 KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct . REFERENCE 1 (bases 1 to 6336) AUTHORS Chang P, Wang W, Chen J. TITLE An efficient vector system for economical and convenient epitope-tagging and promoters switching in Candida albicans JOURNAL Unpublished REFERENCE 2 (bases 1 to 6336) AUTHORS Chang P, Wang W, Chen J. TITLE Direct Submission REFERENCE 3 (bases 1 to 6336) TITLE Direct Submission REFERENCE 4 (bases 1 to 6336) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (31-JAN-2018) Research Center of Bioenergy " SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..6336 /mol_type="other DNA" /organism="synthetic DNA construct" CDS complement(754..1611) /label="AmpR" /note="beta-lactamase" promoter complement(1612..1716) /label="AmpR promoter" promoter 4108..4126 /label="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" primer_bind 4159..4175 /label="SK primer" /note="common sequencing primer, one of multiple similar variants" CDS 4652..5461 /gene="URA3" /label="Orotidine 5'-phosphate decarboxylase" /note="Orotidine 5'-phosphate decarboxylase from Candida albicans (strain SC5314 / ATCC MYA-2876). Accession#: P13649" protein_bind complement(5612..5645) /label="loxP" /note="Cre-mediated recombination occurs in the 8-bp core sequence (ATGTATGC) (Shaw et al., 2021)." rep_origin complement(join(6328..6336,1..580)) /direction=LEFT /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
This page is informational only.