LOCUS       V008269                 6336 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008269
VERSION     V008269
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6336)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6336)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6336)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6336)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-JAN-2018) Research Center of Bioenergy "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6336
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(754..1611)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1612..1716)
                     /label="AmpR promoter"
     promoter        4108..4126
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     4159..4175
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             4652..5461
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"
     protein_bind    complement(5612..5645)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(join(6328..6336,1..580))
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
       61 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
      121 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
      181 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
      241 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
      301 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
      361 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
      421 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
      481 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
      541 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
      601 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
      661 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
      721 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
      781 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
      841 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
      901 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
      961 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     1021 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     1081 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     1141 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     1201 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     1261 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     1321 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     1381 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     1441 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     1501 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     1561 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     1621 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     1681 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     1741 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     1801 gccaggtcga ctctagacat tggtccatcg agaatgagat aaagctattc agcttattgt
     1861 gcgattataa gcccgctggt aaaaacaaac aacacaatat taaaatcatt atcaacaata
     1921 ttaatgaatc tttagactct aaggaggaac ccttcagcga atatgacgtg tggaataaat
     1981 tgaggtcttt atatgatcta gaaaagattg accagattga ggaattactg actgagaaca
     2041 ccagtacaga agaaaccaca aaccaagtgc tgtctggatc tgcaaatatc acgaaggaca
     2101 ccaagaacgc taaaactact ttggatgcaa aagagccaat tcaagataag aaagaaatgg
     2161 ataaagagaa atcagaatcg tcatcattgt ctacccctga accaccacaa agaagaacta
     2221 gaagtgccag agatacaaca aaatcgaaac gtaacttgcg tgcgatccgt ctaggattga
     2281 acgatgatat taagcgagaa ttcacatttt tctagaggaa tcacagttac aattgtagag
     2341 gaaaccgaac tagtaaaact agcacccaac acttctaaac aaaccaatca attcaatggt
     2401 ataacaaatc gtctaccaga agttatgcta atatgaaaca tcaattgaca acatggacat
     2461 caactgaagc tttaacaaat gaataaaaaa aaaagcccat acaatagacc attggtcata
     2521 gtttcatttg cccccgcccc acttttattg ttgagttgaa taattgtgag catcagctga
     2581 ttttaaaatt ataaattttt tttttcgtcc attgttaatt ctattttagt tttttcttct
     2641 ccatcgtctc cgaattattc cggaagctgg tagcggataa gttttagaat cattgagcct
     2701 cttttcaatg ccagatgtta tctgcaactg atggttagtt agttagtata agacttaaat
     2761 tctcatatag gtcatgtgca cggacaagct cattgagtga cgaaaagtca aatttaaact
     2821 tctctaaaca aatttcagac aatagtccga aaaaaaaaaa caaaacccat tattgtcgac
     2881 atgtcaaagg attcaacggt tgagatggag ccgtcaaata tccaaaacac tcttctttca
     2941 actaatttgc cgtaaactat ctccatcgta cgattaagat aaaaaaaaac aattatttct
     3001 tacaatattt gatagagacc caatgcaaag ccagcgagaa agaggagtat tggcattgtt
     3061 gggtagtaga tgaatgagta atcttctagc caaaacaaaa taaaccacaa gaacagtagg
     3121 caaaactgag tacttgatag cgcttgtagt ctttgtattt aaaaaaacct aattgtccaa
     3181 aaattgaaaa tcatgagttg caaatttgtt gaaatcacca ggtaaataga tcatttagct
     3241 atatcgcctt atcattcttc gtacaagaat tatacacaaa taccaccacc acaccaccca
     3301 gaaattttac acgtgttttc taaattatta ctttccgtgg ccaatcaaat tgaaattttt
     3361 cttgttcgaa acggcagtgc ctcaaacatt atagtagcaa gtattgccta aagagtttcc
     3421 gtctcgtgcc ccccccctat ccccttttgt aatttgccaa aatagctcaa aaaggataat
     3481 cccgcacttt ttttttccga gttctcgtca tgcaagcaag cttattcaga attttcagag
     3541 gtgcgagtct caccactacc actgcagctg catcttttac agcaacagca acagcaggtg
     3601 ccaccacggc aaagacattg tctggatcca ctgtgctccg aaaactgtat aaaaggacct
     3661 attcatccct ggtcttatct tctccagaat tatttttttt tcatcagttt aacaacaaca
     3721 aacgttattg tcatacaaca acaacaacaa ccgtgcatcg ctaccattgt tcaccacaaa
     3781 tgtttctaag agtgaaattc tggaaatctg gaaatctggt tttgtattct tgttattctt
     3841 ctttttgtta ttacatatat aacttgttac ttttttaaaa aaatctttgt ttattttata
     3901 aatatataaa actaaattta agaaaaagag aaaaatgttt tatttgagag attgaaattt
     3961 tacttgaatt tagcttagct tttataaagt attattatgt aaaaaaacaa aacaaatata
     4021 cattaaaaag ttaagactat aaaatagcca cccaaggcat ttctatatct acagtctgcc
     4081 agacttaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga attggagctc
     4141 caccgcggtg gcggccgctc tagaactagt ggatccagat cttgatccat aacttcgtat
     4201 agcatacatt atacgaagtt atagatccag tactaatagg aattgatttg gatggtataa
     4261 acggaaacaa aaaaaagagc tggtactact ttctttaaaa ttattttatt atttgatttt
     4321 atttaatagt atatattata ttttgaacgt agattatttt gttgaaagtt gctgtagtgc
     4381 cattgattcg taacactaat tctgtattag tcattcctct tgtttgatag tatccaaaaa
     4441 aacggctatt tttttgcaat cttatttcct gcatattata cagataacat aatgaaagaa
     4501 aaaatctttt tttttgttct tcaatgatga tttcaaccat tcttttaaac attgatcaat
     4561 tcctgagcaa caaccccata cacactggtt tatataccgc cccttttaca gttgaagaaa
     4621 gaaatagaaa tagaaatagc aaacaaaaga tatgacagtc aacactaaga cctatagtga
     4681 gagagcagaa actcatgcct caccagtagc acagcgatta tttcgattaa tggaactgaa
     4741 gaaaaccaat ttatgtgcat caattgacgt tgataccact aaggaattcc ttgaattaat
     4801 tgataaatta ggtccttatg tatgcttaat caagactcat attgatataa tcaatgattt
     4861 ttcctatgaa tccactattg aaccattatt agaactttca cgtaaacatc aatttatgat
     4921 ttttgaagat agaaaatttg ctgatattgg taataccgta aagaaacaat atattggtgg
     4981 agtttataaa attagtagtt gggcagatat taccaatgct catggtgtca ctgggaatgg
     5041 agtggttgaa ggattaaaac agggagctaa agaaaccacc accaaccaag agccaagagg
     5101 gttattgatg ttagctgaat tatcatcagt gggatcatta gcatatggag aatattctca
     5161 aaaaactgtt gaaattgcta aatccgataa ggaatttgtt attggattta ttgcccaacg
     5221 tgatatgggt ggccaagaag aaggatttga ttggcttatt atgacacctg gagttggatt
     5281 agatgataaa ggtgatggat taggacaaca atatagaact gttgatgaag ttgttagcac
     5341 tggaactgat attatcattg ttggtagagg attgtttggt aaaggaagag atccagatat
     5401 tgaaggtaaa aggtatagaa atgctggttg gaatgcttat ttgaaaaaga ctggccaatt
     5461 ataaatgtga agggggagat tttcacttta ttagatttgt atatatgtag aataaataaa
     5521 taaataagtt aaataaataa ttaaataagg gtggtaatta ttactattta caatcaaagg
     5581 tggtccttct agaagatctg ctagcccatg gataacttcg tatagcatac attatacgaa
     5641 gttattaggg ataacagggt aatctgcagg aattcgcagt cagtcagtca gcaaagataa
     5701 gggggatgag acaattgcta aaattaagaa agaaaacact tctgagcaag aagaagataa
     5761 aaacaaaaag cagacagaag aagaggcaga agaagaggaa gaagaaggtg atcatgttag
     5821 tggagatgaa agggaaagca aaaataagga cactagtgaa gatgaggatg gtgaagataa
     5881 tgaagatcaa gatgaagatg atgttgaagt aattgaggac agccatactg ataaagaaaa
     5941 tgaatctagt tcagaagaat catcacctga accaatgact aaacgaacga gacaaagaac
     6001 aaggtccacc catgaaaaaa cagaacacac gtcacctaat atcaaaaaga gaacaagaca
     6061 gagtgtcaaa tttgacgaca agcctgcaga gccctccgat acaggttcta gaaaaagacg
     6121 agctccgcca ggatcagtat ctcctccaca tcctcctaaa acacgaagac gtacaagatc
     6181 agtaacccat gaaatagagg aagaggatgc ttcgacagta atcatggtca taccgcggag
     6241 gcctgggccc gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     6301 ccgtaaaaag gccgcgttgc tggcgttttt ccatag
//
