pCOS5 vector (V008286)

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LOCUS       V008286                 7286 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008286
VERSION     V008286
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7286)
  AUTHORS   Connell TD, Martone AJ, Holmes RK.
  TITLE     A new mobilizable cosmid vector for use in Vibrio cholerae and other
            gram-negative bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7286)
  AUTHORS   Connell TD.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-1994) Terry D. Connell, University at Buffalo,
            Department of Microbiology, 3435 Main Street, Buffalo, NY 14214, USA
REFERENCE   3  (bases 1 to 7286)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7286)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-AUG-1994) Terry D. Connell, University at Buffalo, Department of
            Microbiology, 3435 Main Street, Buffalo, NY 14214, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7286
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            complement(37..144)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             complement(531..1187)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(1188..1290)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     terminator      2299..2393
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(3732..4274)
                     /gene="Nu1"
                     /label="Terminase small subunit"
                     /note="Terminase small subunit from Escherichia phage
                     lambda. Accession#: P03707"
     CDS             4841..5029
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    5134..5274
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5460..6048)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6221..7078)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7079..7183)
                     /label="AmpR promoter"