LOCUS       V008286                 7286 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008286
VERSION     V008286
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7286)
  AUTHORS   Connell TD, Martone AJ, Holmes RK.
  TITLE     A new mobilizable cosmid vector for use in Vibrio cholerae and other
            gram-negative bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7286)
  AUTHORS   Connell TD.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-1994) Terry D. Connell, University at Buffalo,
            Department of Microbiology, 3435 Main Street, Buffalo, NY 14214, USA
REFERENCE   3  (bases 1 to 7286)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7286)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-AUG-1994) Terry D. Connell, University at Buffalo, Department of
            Microbiology, 3435 Main Street, Buffalo, NY 14214, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7286
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            complement(37..144)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             complement(531..1187)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(1188..1290)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     terminator      2299..2393
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(3732..4274)
                     /gene="Nu1"
                     /label="Terminase small subunit"
                     /note="Terminase small subunit from Escherichia phage
                     lambda. Accession#: P03707"
     CDS             4841..5029
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    5134..5274
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5460..6048)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6221..7078)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7079..7183)
                     /label="AmpR promoter"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgataa gcttatcggc cagcctcgca ggcaggattc
       61 ccgttgagca ccgccaggtg cgaataaggg acagtgaaga aggaacaccc gctcgcgggt
      121 gggcctactt cacctatcct gcccggctga cgccgttgga tacaccaagg aaagtctaca
      181 cgaacccttt ggcaaaatcc tgtatatcgt gcgaaaaagg atggatatac cgaaaaaatt
      241 cgctataatg accccgaagc agggttatgc agcggaaaag atccgtcgac ctgcagccaa
      301 ggacggatcc tctagagtcg gcatttgaga agcacacggt cacactgctt ccggtagtca
      361 ataaaccggt aaaccagcaa tagacataag cggctattta acgaccctgc cctgaaccga
      421 cgaccgggtc gaatttgctt tcgaatttct gccattcatc cgcttattat cacttattca
      481 ggcgtagcac caggcgttta agggcaccaa taactgcctt aaaaaaatta cgccccgccc
      541 tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca
      601 cagacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa
      661 tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca
      721 aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct
      781 ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga
      841 aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca
      901 tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt
      961 gccatacgga attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga
     1021 taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg
     1081 gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc
     1141 cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta
     1201 gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg
     1261 tgaaagttgg aacctcttac gtgccgatca acgtctcatt ttcgccaaaa gttggacctg
     1321 caggcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg
     1381 cttcctaatg caggagtcgc ataagggaga gcgtcgaccg atgcccttga gagccttcaa
     1441 cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt
     1501 cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca ttttcggcga
     1561 ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat tcggaatctt
     1621 gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca
     1681 ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg cgttcgcgac
     1741 gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc
     1801 cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcagggac agcttcaagg
     1861 atcgctcgcg gctcttacca gcctaacttc gatcactgga ccgctgatcg tcacggcgat
     1921 ttatgccgcc tcggcgagca catggaacgg gttggcatgg attgtaggcg ccgccctata
     1981 ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat
     2041 ggaagccggc ggcacctcgc taacggattc accactccaa gaattggagc caatcaattc
     2101 ttgcggagaa ctgtgaatgc gcaaaccaac ccttggcaga acatatccat cgcgtccgcc
     2161 atctccagca gccgcacgcg gcgcatctcg ggcagcgttg ggtcctggcc acgggtgcgc
     2221 atgatctgcc acattacgct cctgtccggc aaagttacct ctgccgaagt tgagtatttt
     2281 tgctgtattt gtcataatga ctcctgttga tagatccagt aatgacctca gaactccatc
     2341 tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag aatcgcagca
     2401 acttgtcgcg ccaatcgagc catgtcgtcg tcaacgaccc cccattcaag aacagcaagc
     2461 agcattgaga actttggaat ccagtccctc ttccacctgc tgatctgcga cttatcaacg
     2521 ccgacagctt ccgctgtctt ctcagttcca agcattgcga ttttgttaag caacgcactc
     2581 tcgattcgta gagcctcgtt gcgtttgttt gcacgaacca tatgtaagta tttccttaga
     2641 taacaattga ttgaatgtat gcaaataaat gcatacacca taggtgtggt ttaatttgat
     2701 gccctttttc agggctggaa tgtgtaagag cgggttattt atgctgttgt ttttttgtta
     2761 ctcgggaagg gctttacctc ttccgcataa acgcttccat cagcgtttat agttaaaaaa
     2821 atctttcggc ctgcatgaat ggccttgttg atcgcgattt gatatacgcc gagatctaac
     2881 gggcatcagt aaagtccagc tcctgctggc ggatgacgca tgcattatgc tcgcagagat
     2941 aaaacacgct ggaggggtca tccggcgtcc atttgaggcc aaacggcgtc tctttgtcgc
     3001 caaatttaag atactgctcc tccccgcaat gcgggcaggc aacatgaaaa cgcataaaat
     3061 gcggggattc actggctgca cgctcaatct gacaggtgcc tctcactttt ggcgtggagc
     3121 cacggatgga ctttggccag accgagcctt caatacgctt gtcacccagg aacgtcggag
     3181 agccttcctg ttcaatatca tcatcaaaag cagcaagttc atcataaccc gccacatcca
     3241 ccgacttttc acggtagttt tttgccgctt taccgcccag gcaccagaag ccacgcccat
     3301 tagtgaaacg cttcatggtg agcgtgttaa cccggtgctt tttgccatac cacggggcca
     3361 gcgccagcag cgacggaata tcacgaatag tcggctcaac gtgggttttc ataaagttct
     3421 cggcatcacc atccgtcggc aaccagataa gggtgttgcg ctgcttatgc tctataaagt
     3481 aggcataaac acccagcagc attttggaat aaccgacacg ggcagacttc accacattca
     3541 cctcacggat gtagtcgctg cccatcgcat tcatgatggc ccgctgaaag ggcagtgttt
     3601 cccagcgccc ttcctggtat gcggattctt tcgggagata gtaattagca tccgcccatt
     3661 caacggcggt ctgtggctcc ggcctgaaca gtgagcgaag cccggcgcgg acaaaatgcc
     3721 gcagcctgtt atcctgactg ttcgatatat tcactcagca accccggtat cagttcatcc
     3781 agcgcggctg ctttgttcat ggctttgatg atatcccgtt tcaggaaatc aacatgtcgg
     3841 ttttccagtt ccggaaaacg ccgctgcacc gacaggggga gcccgtcgag aatactggca
     3901 atttcacctg cgatccgcga cagcacgaaa gtacagaatg cggttaccac cacttcagcg
     3961 gagtctctgg cattcttcag ttcctgtgcg tcggcctgcg cacgcgtaag tcgatggcgt
     4021 tcgtactcaa tagttcctgg ctggggatct gcctcgctgg cctgccgcag ttcttcaacc
     4081 tcccggcgca gcttttcgtt ctcaatttca gcatcccttt cggcatacca ttttatgacg
     4141 gcggcagagt cataaagcac ctcattaccc ttgccaccgc ctcgcagaac gggcattccc
     4201 tgttcctgcc agttctgaat ggtacggata ctcgcaccga aaatgtcagc cagctgcttt
     4261 ttgttgactt ccattgttca ttccacggac aaaaacagag aaaggaaacg acagaggcca
     4321 aaaagcctcg ctttcagcac ctgtcgtttc ctttcttttc agagggtatt ttaaataaaa
     4381 acattaagtt atgacgaaga agaacggaaa cgccttaaac cggaaaattt tcataaatag
     4441 cgaaaacccg cgaggtcgcc gccccgtaac ctgtcggatc accggaaagg acccgtaaag
     4501 tgataatgat tatcatctac atatcacaac gtgcgtggag gccatcaaac cacgtcaaat
     4561 aatcaattat gacgcaggta tcgtattaat agatctgcat cgcaggatgc tgctggctac
     4621 cctgtggaac acctacatct gtattaacga agcgctggca ttgaccctga gtgatttttc
     4681 tctggtcccg ccgcatccat accgccagtt gtttaccctc acaacgttcc agtaaccggg
     4741 catgttcatc atcagtaacc cgtatcgtga gcatcctctc tcgtttcatc ggtatcatta
     4801 cccccatgaa cagaaattcc cccttacacg gaggcatcaa gtgaccaaac aggaaaaaac
     4861 cgcccttaac atggcccgct ttatcagaag ccagacatta acgcttctgg agaaactcaa
     4921 cgagctggac gcggatgaac aggcagacat ctgtgaatcg cttcacgacc acgctgatga
     4981 gctttaccgc agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     5041 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     5101 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga
     5161 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac
     5221 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct
     5281 tccgcttcct cgctcactga ctcgctgctc tcggtcgttc ggctgcggcg agcggtatca
     5341 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     5401 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5461 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     5521 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     5581 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     5641 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     5701 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     5761 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     5821 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     5881 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     5941 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6001 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     6061 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaggg attttggtca
     6121 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     6181 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     6241 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     6301 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     6361 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     6421 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     6481 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca
     6541 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     6601 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     6661 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     6721 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     6781 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg
     6841 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     6901 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     6961 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     7021 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     7081 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     7141 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     7201 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     7261 tcacgaggcc ctttcgtctt caagaa
//
