pCONINT vector (V008289)

Basic Vector Information

Vector Name:
pCONINT
Antibiotic Resistance:
Kanamycin
Length:
3999 bp
Type:
Integrative shuttle vector
Replication origin:
ori
Source/Author:
Dennehy M, Bourn W, Steele D, Williamson AL.

pCONINT vector Map

pCONINT3999 bp60012001800240030003600KanRoriIntegraserrnB T1 terminator

pCONINT vector Sequence

LOCUS       V008289                 3999 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008289
VERSION     V008289
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Dennehy M, Bourn W, Steele D, Williamson AL.
  TITLE     Evaluation of recombinant BCG expressing rotavirus VP6 as an
            anti-rotavirus vaccine
  JOURNAL   Vaccine 25 (18), 3646-3657 (2007)
   PUBMED   17339069
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Bourn W.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2005) Medical Virology, University of Cape Town,
            Anzio Road, Observatory, Cape Town 7925, South Africa
REFERENCE   3  (bases 1 to 3999)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3999)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Vaccine";
            date: "2007"; volume: "25"; issue: "18"; pages: "3646-3657"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (27-AUG-2005) Medical Virology, University of Cape Town, Anzio Road,
            Observatory, Cape Town 7925, South Africa"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3999
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             120..932
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1267..1855
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             2580..3692
                     /gene="33"
                     /label="Integrase"
                     /note="Integrase from Mycobacterium phage L5. Accession#:
                     P22884"
     terminator      3917..3963
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"

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