LOCUS       V008289                 3999 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008289
VERSION     V008289
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Dennehy M, Bourn W, Steele D, Williamson AL.
  TITLE     Evaluation of recombinant BCG expressing rotavirus VP6 as an
            anti-rotavirus vaccine
  JOURNAL   Vaccine 25 (18), 3646-3657 (2007)
   PUBMED   17339069
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Bourn W.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2005) Medical Virology, University of Cape Town,
            Anzio Road, Observatory, Cape Town 7925, South Africa
REFERENCE   3  (bases 1 to 3999)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3999)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Vaccine";
            date: "2007"; volume: "25"; issue: "18"; pages: "3646-3657"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (27-AUG-2005) Medical Virology, University of Cape Town, Anzio Road,
            Observatory, Cape Town 7925, South Africa"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3999
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             120..932
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1267..1855
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             2580..3692
                     /gene="33"
                     /label="Integrase"
                     /note="Integrase from Mycobacterium phage L5. Accession#:
                     P22884"
     terminator      3917..3963
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 gctagcaaca aagcgacgtt gtgtctcaaa atctctgatg ttacattgca caagataaaa
       61 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta
      121 tgagccatat tcaacgggaa acgtcttgct cgaggccgcg attaaattcc aacatggatg
      181 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct
      241 atcgcttgta tgggaagccc catgcgccag agttgtttct gaaacatggc aaaggtagcg
      301 ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc
      361 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga
      421 tccccgggaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg
      481 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt
      541 ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg
      601 ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag
      661 aaatgcataa tcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac
      721 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg
      781 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc
      841 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat
      901 tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac
      961 actggcagag cattacgctg acttgacggg acggcggctt tgttgaataa atcgaacttt
     1021 tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa
     1081 gcaaaagttc aaaatcacca actggtccac ctacaacaaa gctctcatca accgtggctc
     1141 cctcactttc tggctggatg atggggcgat tcaggcctgg tatgagtcag caacaccttc
     1201 ttcacgaggc agacctcact agttccactg agcgtcagac cccgtagaaa agatcaaagg
     1261 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1321 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1381 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     1441 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1501 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1561 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     1621 aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc
     1681 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     1741 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     1801 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     1861 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     1921 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     1981 cgctcgccgc agccgaacga ccgagcgcaa cgcgtcgacc accaagggca ccatctctgc
     2041 ttgggccacc ccgttggccg cagccagctc gctgagagcc gtgaacgaca gggcgaacgc
     2101 cagcccgccg acggcgaggg ttccgaccgc tgcaactccc ggtgcaacct tgtcccggtc
     2161 tattctcttc actgcaccag ctccaatctg gtgtgaatgc ccctcgtctg ttcgcgcagg
     2221 cggggggctc tattcgtttg tcagcatcga aagtagccag atcagggatg cgttgcaacc
     2281 gcgtatgccc aggtcagaag agtcgcacaa gagttgcaga cccctggaaa gaaaaatggc
     2341 cagagggcga aaacaccctc tgaccagcgg agcgggcgac gggaatcgaa cccgcgtagc
     2401 tagtttggaa gaatgggtgt ctgccgacca catatgggcc ggtcaagata ggtttttacc
     2461 ccctctcggc tgcatcctct aagtggaaag aaattgcagg tcgtagaagc gcgttgaagc
     2521 ctgagagttg cacaggagtt gcaacccggt agccttgttc acgacgagag gagacctagt
     2581 tggcacgtcg cggatgggga tcgctgaaga ctcagcgcag cgggaggatc caagcctcat
     2641 acgtcaaccc gcaggacggt gtgaggtact acgcgctgca gacctacgac aacaagatgg
     2701 acgccgaagc ctggctcgcg ggcgagaagc ggctcatcga gatggagacc tggacccctc
     2761 cacaggaccg ggcgaagaag gcagccgcca gcgccatcac gctggaggag tacacccgga
     2821 agtggctcgt ggagcgcgac ctcgcagacg gcaccaggga tctgtacagc gggcacgcgg
     2881 agcgccgcat ctacccggtg ctaggtgaag tggcggtcac agagatgacg ccagctctgg
     2941 tgcgtgcgtg gtgggccggg atgggtagga agcacccgac tgcccgccgg catgcctaca
     3001 acgtcctccg ggcggtgatg aacacagcgg tcgaggacaa gctgatcgca gagaacccgt
     3061 gccggatcga gcagaaggca gccgatgagc gcgacgtaga ggcgctgacg cctgaggagc
     3121 tggacatcgt cgccgctgag atcttcgagc actaccggat cgcggcatac atcctggcgt
     3181 ggacgagcct ccggttcgga gagctgatcg agcttcgccg caaggacatc gtggacgacg
     3241 gcatgacgat gaagctccgg gtgcgccgtg gcgcttcccg cgtggggaac aagatcgtcg
     3301 ttggcaacgc caagaccgtc cggtcgaagc gtcctgtgac ggttccgcct cacgtcgcgg
     3361 agatgatccg agcgcacatg aaggaccgta cgaagatgaa caagggcccc gaggcattcc
     3421 tggtgaccac gacgcagggc aaccggctgt cgaagtccgc gttcaccaag tcgctgaagc
     3481 gtggctacgc caagatcggt cggccggaac tccgcatcca cgacctccgc gctgtcggcg
     3541 ctacgttcgc cgctcaggca ggtgcgacga ccaaggagct gatggcccgt ctcggtcaca
     3601 cgactcctag gatggcgatg aagtaccaga tggcgtctga ggcccgcgac gaggctatcg
     3661 ctgaggcgat gtccaagctg gccaagacct cctgaaacgc aaaaagcccc cctcccaagg
     3721 acactgagtc ctaaagaggg gggtttcttg tcagtacgcg aagaaccacg cctggccgcg
     3781 agcgccagca ccgccgctct gtgcggagac ctgggcacca gccccgccgc cgccaggagc
     3841 attgccgttc ccgccagaaa tctagagctt atcgatgtcg acgtagttaa ctagcgtacg
     3901 atcgactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     3961 tatctgttgt ttgtccggcc atcatggccg cggtgatca
//
