pCST5-del-URA3 vector (V008131)

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LOCUS       V008131                 6271 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008131
VERSION     V008131
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6271)
  AUTHORS   Cote P, Sulea T, Dignard D, Wu C, Whiteway M.
  TITLE     Evolutionary reshaping of fungal mating pathway scaffold proteins
  JOURNAL   MBio 2 (1), e00230-10 (2011)
   PUBMED   21249169
REFERENCE   2  (bases 1 to 6271)
  AUTHORS   Cote P.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2010) Health Sector, CNRC-BRI, 6100 Royalmount
            Av., Montreal, QC H4P2R2, Canada
REFERENCE   3  (bases 1 to 6271)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6271)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio";
            date: "2011"; volume: "2"; issue: "1"; pages: "e00230-10"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-NOV-2010) Health Sector, CNRC-BRI, 6100 Royalmount Av.,
            Montreal, QC H4P2R2, Canada"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6271
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             411..1204
                     /codon_start=1
                     /product="Cst5"
                     /label="Cst5"
                     /note="5' end disruption cassette"
                     /protein_id="ADW40659.1"
                     /translation="MLQSTPKSWKDFLKSPKKPTSPTTPPGSVTITPNTTPPSVPCVSP
                     RPAKIPSRDPFQTINPGLNLSFANILNKSLANHETCFICGELLSTVFASERILKLNCGD
                     STHSECFKAYFKEDIPHARIHGKTITFAKTCRGLNCSGTRNVVVDVNNWHLVSARKLSL
                     IPKRPAPPHPNSVNINALKTTLQNSDIPTRSPSPDPTVSTTTEVDAYEVETVRNQLIKY
                     LLDLCPKINLSRLVLLGNLRVADELSVCVEPLDYFQTRYVYL"
     primer_bind     complement(1249..1265)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(1269..2078)
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"
     promoter        complement(2915..2933)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2954..2970)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    2978..2994
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3002..3032)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    3047..3068
                     /label="CAP binding site"
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             3078..3435
                     /codon_start=1
                     /product="Cst5"
                     /label="Cst5"
                     /note="3' end disruption cassette"
                     /protein_id="ADW40660.1"
                     /translation="RKLHGYME*C*LPCIRPYAEYPNLLSWIVHPTSASKR*WVIDIDS
                     IYI*HSSGKMGCCHF*RSFTVASTGYNVYN*HLP****FRY*FRRRSYSASTN*KQLGR
                     FND*D**CVTYI*PV"
     primer_bind     complement(4033..4049)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        complement(4086..4104)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4125..4141)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4149..4165)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4173..4203)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4218..4239)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4527..5115)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5289..6146)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6147..6251)
                     /label="AmpR promoter"