LOCUS       V008131                 6271 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008131
VERSION     V008131
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6271)
  AUTHORS   Cote P, Sulea T, Dignard D, Wu C, Whiteway M.
  TITLE     Evolutionary reshaping of fungal mating pathway scaffold proteins
  JOURNAL   MBio 2 (1), e00230-10 (2011)
   PUBMED   21249169
REFERENCE   2  (bases 1 to 6271)
  AUTHORS   Cote P.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2010) Health Sector, CNRC-BRI, 6100 Royalmount
            Av., Montreal, QC H4P2R2, Canada
REFERENCE   3  (bases 1 to 6271)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6271)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio";
            date: "2011"; volume: "2"; issue: "1"; pages: "e00230-10"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-NOV-2010) Health Sector, CNRC-BRI, 6100 Royalmount Av.,
            Montreal, QC H4P2R2, Canada"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6271
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             411..1204
                     /codon_start=1
                     /product="Cst5"
                     /label="Cst5"
                     /note="5' end disruption cassette"
                     /protein_id="ADW40659.1"
                     /translation="MLQSTPKSWKDFLKSPKKPTSPTTPPGSVTITPNTTPPSVPCVSP
                     RPAKIPSRDPFQTINPGLNLSFANILNKSLANHETCFICGELLSTVFASERILKLNCGD
                     STHSECFKAYFKEDIPHARIHGKTITFAKTCRGLNCSGTRNVVVDVNNWHLVSARKLSL
                     IPKRPAPPHPNSVNINALKTTLQNSDIPTRSPSPDPTVSTTTEVDAYEVETVRNQLIKY
                     LLDLCPKINLSRLVLLGNLRVADELSVCVEPLDYFQTRYVYL"
     primer_bind     complement(1249..1265)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(1269..2078)
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"
     promoter        complement(2915..2933)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2954..2970)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    2978..2994
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3002..3032)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    3047..3068
                     /label="CAP binding site"
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             3078..3435
                     /codon_start=1
                     /product="Cst5"
                     /label="Cst5"
                     /note="3' end disruption cassette"
                     /protein_id="ADW40660.1"
                     /translation="RKLHGYME*C*LPCIRPYAEYPNLLSWIVHPTSASKR*WVIDIDS
                     IYI*HSSGKMGCCHF*RSFTVASTGYNVYN*HLP****FRY*FRRRSYSASTN*KQLGR
                     FND*D**CVTYI*PV"
     primer_bind     complement(4033..4049)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        complement(4086..4104)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4125..4141)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4149..4165)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4173..4203)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4218..4239)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4527..5115)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5289..6146)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6147..6251)
                     /label="AmpR promoter"
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gcctaaatct gaaacatcga ctataaaacc
      361 accaaaaaaa tcccatccat cgtcaatctg ttaatattac ccaccaggca atgctacaat
      421 ctacgcctaa gagctggaaa gactttttaa aatctccaaa gaagcccact agccctacca
      481 ccccaccagg cagtgtgacg atcacaccca atacaacacc tccaagtgtg ccatgtgtca
      541 gtccacgacc agcgaaaatc ccttccagag acccatttca aaccataaat cccggactaa
      601 acctttcctt tgccaatatt ctcaacaagt cactagcaaa tcacgaaaca tgtttcatct
      661 gtggagaact actttctact gtatttgcat cagaacgaat cctaaagtta aattgtggtg
      721 actccaccca cagtgaatgt ttcaaagctt attttaagga agatatacca cacgcaagaa
      781 tccatggcaa gacaataacg tttgccaaaa cctgtcgtgg tttaaattgc agtggcacca
      841 gaaacgtagt tgtcgatgta aacaactggc acttggtttc tgctagaaag ttgtcgttaa
      901 tacccaagag accagcacct ccacacccca acagtgtcaa cattaacgct ttaaagacaa
      961 ccttacaaaa cagcgatata cctaccagat ctccttcacc agacccaact gtttcaacca
     1021 ccacagaagt ggacgcatat gaggttgaaa cggttagaaa ccaactaatc aaatatttgt
     1081 tagatctgtg tcctaaaatc aacttgtcaa gattggttct gttgggaaat ttgcgagttg
     1141 cagacgagct atctgtttgt gtggagccac tggactattt ccaaacaagg tatgtttatt
     1201 tattcgacgg tatcgataag cttgatatcg aattcctgca gcccggggga tccactagtt
     1261 ctagattata attggccagt ttttttcaaa taagcattcc aaccagcatc tctatacctt
     1321 ttaccttcaa tatctggatc tcttccttta ccaaacaatc ctctaccaac aatgataata
     1381 tcagttccag tgctaacaac ttcatcaaca gttctatatt gttgtcctaa tccatcacct
     1441 ttatcatcta atccaactcc aggtgtcata ataagccaat caaatccttc ttcttgtcca
     1501 cccatatcac gttgggcaat aaatccaata acaaattcct tatcggattt agcaatttca
     1561 acagtttttt gagaatattc tccatatgct aatgatccca ctgatgataa ttcagctaac
     1621 atcaataacc ctcttggctc ttggttggtg gtggtttctt tagctccctg ttttaatcct
     1681 tcaactactc cattcccagt gacaccatga gcattagtaa tatctgccca actactaatt
     1741 ttataaactc caccaatata ttgtttcttc acggtattac caatatcagc aaattttcta
     1801 tcttcaaaaa tcataaattg atgtttacgt gaaagttcta ataatggttc aatagtggat
     1861 tcataggaaa aatcattgat tatatcaata tgagtcttga ttaagcatac ataaggaccc
     1921 aatttatcaa ttaattcaag gaattcctta gtggtatcaa catcaattga tgcacataaa
     1981 ttggttttct tcagttccat taatcgaaat aatcgttgtg ctactggtga ggcatgagtt
     2041 tctgctctct cactataggt cttagtgttg actgtcatat cttttgtttg ctatttctat
     2101 ttctatttct ttcttcaact gtaaaagggg cgatatataa accagtgtgt atggggttgt
     2161 tgctcaggaa ttgatcaatg tttaaaagaa tggttgaaat catcattgaa gaacaaaaaa
     2221 aaagattttt tctttcatta tgttatctgt ataatatgca ggaaataaga ttgcaaaaaa
     2281 atagccgttt ttttggatac tatcaaacaa gaggaatgac taatacagaa ttagtgttac
     2341 gaatcaatgg cactacagca actttcaaca aaataatcta cgttcaaaat ataatatata
     2401 ctattaaata aaatcaaata ataaaataat tttaaagaaa gtagtaccag ctcttttttt
     2461 tgtttccgtt tataccatcc aaatcaattc ctattagtac tttgtaatta ctctcagttc
     2521 aaactgttta aataattcat aagttttttt tgaaatggat tttgttctga agatccggtt
     2581 ttggaattgg attttttatt tgaaacacca gactgtgtat ttgttgtggt ggttgcagaa
     2641 ttgttcgtgg gtgttttcaa atctgcatta ttgtcgttcg aatctttacc agcattagta
     2701 atgaaggatt catagttctc accagtcttt cccgtaaaac tatcgacggt ggcagtagaa
     2761 attcgattgg tgttcacatt attggtcatt tcatccgcag taggaatacc agaaacagtc
     2821 gtagcggtag gggtagttgt actgacgtca acactgttaa acttatcatt ggtgacatgc
     2881 ggccgccacc gcggtggagc tccagctttt gttcccttta gtgagggtta atttcgagct
     2941 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     3001 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     3061 tcacattaat tgcgttgcga aaactacatg gttatatgga atagtgttga ctaccctgta
     3121 ttcgtcccta tgcagaatat ccaaatctcc tctcgtggat cgtccatcct acaagtgcgt
     3181 caaaaagatg atgggttatc gacattgatt caatctacat ctagcacagt agtggaaaaa
     3241 tgggttgttg ccatttctga cgctcattta cagttgccag caccggatat aacgtctaca
     3301 attgacacct cccctagtga tagtgattcc gatattgatt cagacgaaga agttattcag
     3361 caagcactaa ctaaaaacaa ttgggccgat ttaatgattg agattgataa tgcgttactt
     3421 acatctgacc cgtaagccga tccatctcac ttaatataca cattgtccat cgatctcttt
     3481 cttgtcgact attagctgta aacataatac tctttcccgt tattcccaac tttctaatca
     3541 ttgttaccat tccaggattt ttttcattct gtacccccac ttccaacagt tttggaagct
     3601 gtttttcgta atgacttatg tttctctttt tgccaaacca taatgtgaaa cataattcac
     3661 aatcttcttc catggatttc cagccatcag ggtagactcg tgggactgaa tgggtatcga
     3721 cctgtgttgt tcgagtttca caattcaaca agtctagttc tgttaattta ccagtgtata
     3781 catagcattg tgacaatgga atagtaatat aatgctcata aaatgctgtc agtttcggag
     3841 ttcctgtccc cctggacctc ctaaaggtgt ggtagataat caaataccca ggacatagca
     3901 caacataaaa gtgattgaaa ctagcgtgtt ttttattctt ctcgtacaaa tacccactct
     3961 taatcaccaa catattaggt agtagatgcg acggtatcga taagcttgat atcgaattcc
     4021 tgcagcccgg gggatccact agttctagag cggccgccac cgcggtggag ctccagcttt
     4081 tgttcccttt agtgagggtt aatttcgagc ttggcgtaat catggtcata gctgtttcct
     4141 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     4201 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     4261 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     4321 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     4381 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     4441 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     4501 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     4561 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     4621 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     4681 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     4741 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     4801 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     4861 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     4921 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     4981 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     5041 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     5101 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     5161 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     5221 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     5281 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     5341 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     5401 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     5461 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     5521 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     5581 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     5641 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     5701 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     5761 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     5821 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     5881 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     5941 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     6001 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     6061 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     6121 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     6181 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     6241 ggggttccgc gcacatttcc ccgaaaagtg c
//
