pCST5-act1 vector (V008133)

LOCUS       V008133                 7724 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008133
VERSION     V008133
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7724)
  AUTHORS   Cote P, Sulea T, Dignard D, Wu C, Whiteway M.
  TITLE     Evolutionary reshaping of fungal mating pathway scaffold proteins
  JOURNAL   MBio 2 (1), e00230-10 (2011)
   PUBMED   21249169
REFERENCE   2  (bases 1 to 7724)
  AUTHORS   Cote P.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2010) Health Sector, CNRC-BRI, 6100 Royalmount
            Av., Montreal, QC H4P2R2, Canada
REFERENCE   3  (bases 1 to 7724)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7724)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio";
            date: "2011"; volume: "2"; issue: "1"; pages: "e00230-10"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-NOV-2010) Health Sector, CNRC-BRI, 6100 Royalmount Av.,
            Montreal, QC H4P2R2, Canada"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7724
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        626..644
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1124..1933
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"
     primer_bind     2060..2076
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(3068..4219)
                     /codon_start=1
                     /product="Cst5"
                     /label="Cst5"
                     /note="similar to Candida albicans STE5"
                     /protein_id="ADW40669.1"
                     /translation="MLQSTPKSWKDFLKSPKKPTSPTTPPGSVTITPNTTPPSVPCVSP
                     RPAKIPSRDPFQTINPGLNLSFANILNKSLANHETCFICGELLSTVFASERILKLNCGD
                     STHSECFKAYFKEDIPHARIHGKTITFAKTCRGLNCSGTRNVVVDVNNWHLVSARKLSL
                     IPKRPAPPHPNSVNINALKTTLQNSDIPTRSPSPDPTVSTTTEVDAYEVETVRNQLIKY
                     LLDLCPKINLSRLVLLGNLRVADELSVCVEPLDYFQTRYVYLFENYMVIWNSVDYPVFV
                     PMQNIQISSRGSSILQVRQKDDGLSTLIQSTSSTVVEKWVVAISDAHLQLPAPDITSTI
                     DTSPSDSDSDIDSDEEVIQQALTKNNWADLMIEIDNALLTSDP"
     regulatory      complement(4427..5450)
                     /label="ACT1"
                     /note="ACT1"
                     /regulatory_class="promoter"
     promoter        complement(5483..5501)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5522..5538)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5546..5562)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5570..5600)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5615..5636)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5924..6512)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(7231..7609)
                     /label="ISS"
                     /note="immunostimulatory sequence from the AmpR gene;
                     contains unmethylated CpG dinucleotides in the context of
                     5'-AACGTT-3' (Sato et al., 1996)"