LOCUS       V008133                 7724 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008133
VERSION     V008133
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7724)
  AUTHORS   Cote P, Sulea T, Dignard D, Wu C, Whiteway M.
  TITLE     Evolutionary reshaping of fungal mating pathway scaffold proteins
  JOURNAL   MBio 2 (1), e00230-10 (2011)
   PUBMED   21249169
REFERENCE   2  (bases 1 to 7724)
  AUTHORS   Cote P.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2010) Health Sector, CNRC-BRI, 6100 Royalmount
            Av., Montreal, QC H4P2R2, Canada
REFERENCE   3  (bases 1 to 7724)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7724)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio";
            date: "2011"; volume: "2"; issue: "1"; pages: "e00230-10"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-NOV-2010) Health Sector, CNRC-BRI, 6100 Royalmount Av.,
            Montreal, QC H4P2R2, Canada"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7724
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        626..644
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1124..1933
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"
     primer_bind     2060..2076
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(3068..4219)
                     /codon_start=1
                     /product="Cst5"
                     /label="Cst5"
                     /note="similar to Candida albicans STE5"
                     /protein_id="ADW40669.1"
                     /translation="MLQSTPKSWKDFLKSPKKPTSPTTPPGSVTITPNTTPPSVPCVSP
                     RPAKIPSRDPFQTINPGLNLSFANILNKSLANHETCFICGELLSTVFASERILKLNCGD
                     STHSECFKAYFKEDIPHARIHGKTITFAKTCRGLNCSGTRNVVVDVNNWHLVSARKLSL
                     IPKRPAPPHPNSVNINALKTTLQNSDIPTRSPSPDPTVSTTTEVDAYEVETVRNQLIKY
                     LLDLCPKINLSRLVLLGNLRVADELSVCVEPLDYFQTRYVYLFENYMVIWNSVDYPVFV
                     PMQNIQISSRGSSILQVRQKDDGLSTLIQSTSSTVVEKWVVAISDAHLQLPAPDITSTI
                     DTSPSDSDSDIDSDEEVIQQALTKNNWADLMIEIDNALLTSDP"
     regulatory      complement(4427..5450)
                     /label="ACT1"
                     /note="ACT1"
                     /regulatory_class="promoter"
     promoter        complement(5483..5501)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5522..5538)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5546..5562)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5570..5600)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5615..5636)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5924..6512)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(7231..7609)
                     /label="ISS"
                     /note="immunostimulatory sequence from the AmpR gene;
                     contains unmethylated CpG dinucleotides in the context of
                     5'-AACGTT-3' (Sato et al., 1996)"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggtggc ggccgcggcc gacttggcca agcctagatc ccgactaata ggaattgatt
      721 tggatggtat aaacggaaac aaaaaaaaga gctggtacta ctttctttaa aattatttta
      781 ttatttgatt ttatttaata gtatatatta tattttgaac gtagattatt ttgttgaaag
      841 ttgctgtagt gccattgatt cgtaacacta attctgtatt agtcattcct cttgtttgat
      901 agtatccaaa aaaacggcta tttttttgca atcttatttc ctgcatatta tacagataac
      961 ataatgaaag aaaaaatctt tttttttgtt cttcaatgat gatttcaacc attcttttaa
     1021 acattgatca attcctgagc aacaacccca tacacactgg tttatatacc gcccctttta
     1081 cagttgaaga aagaaataga aatagaaata gcaaacaaaa gatatgacag tcaacactaa
     1141 gacctatagt gagagagcag aaactcatgc ctcaccagta gcacagcgat tatttcgatt
     1201 aatggaactg aagaaaacca atttatgtgc atcaattgac gttgatacca ctaaggaatt
     1261 ccttgaatta attgataaat taggtcctta tgtatgctta atcaagactc atattgatat
     1321 aatcaatgat ttttcctatg aatccactat tgaaccatta ttagaacttt cacgtaaaca
     1381 tcaatttatg atttttgaag atagaaaatt tgctgatatt ggtaataccg taaagaaaca
     1441 atatattggt ggagtttata aaattagtag ttgggcagat attaccaatg ctcatggtgt
     1501 cactgggaat ggagtggttg aaggattaaa acagggagct aaagaaacca ccaccaacca
     1561 agagccaaga gggttattga tgttagctga attatcatca gtgggatcat tagcatatgg
     1621 agaatattct caaaaaactg ttgaaattgc taaatccgat aaggaatttg ttattggatt
     1681 tattgcccaa cgtgatatgg gtggccaaga agaaggattt gattggctta ttatgacacc
     1741 tggagttgga ttagatgata aaggtgatgg attaggacaa caatatagaa ctgttgatga
     1801 agttgttagc actggaactg atattatcat tgttggtaga ggattgtttg gtaaaggaag
     1861 agatccagat attgaaggta aaaggtatag aaatgctggt tggaatgctt atttgaaaaa
     1921 gactggccaa ttataaatgt gaagggggag attttcactt tattagattt gtatatatgt
     1981 agaataaata aataaataag ttaaataaat aattaaataa gggtggtaat tattactatt
     2041 tacaatcaaa ggtggtcctt ctagaactag tggatccccc agatcattat ccttaaacag
     2101 attctaaaac aacatctttg aaaccagaag aaactttctt acctttttct tcggttgaac
     2161 cttcaccgtg caaagccaat aatgaacccc agtcgaattt tggttgtttc aacaatttga
     2221 cttttctgat gtggacattt tgtaatggga aaatggtttg gggggatttt tcaatttcac
     2281 ggccaatgac ttctggaatc aatttggaag ttaattgagc taaagtcagt tggaaacttc
     2341 tctttgcata atttcaatca tcttctttct gacttctctc aatttagagg attgagcgta
     2401 agtagtttcc ttgatttggt ttggttgtct tttggtgaaa gcaatggcaa aaactctcaa
     2461 aacgtaatcg tcggaagttt taacagtgac attagcttca actaatgatt gccattttct
     2521 gaccaatgat cttaatttgt cagaagtgaa atccaaacca tgaggcctcc atgaggcctc
     2581 catgaggcct ccatgaggcc tccatggagg cctcatggaa gttggtcaac aagtttttac
     2641 cttggacttc atcaactctc aatttgattt ttctgtaaga gtggtcttcg gaaccttgta
     2701 agtcggccaa acaaacttcg aaaactctac ctttcaagcc atcagcggca ttctttaaac
     2761 cggtagatct gttgatcaaa gttttaccaa catttctgtt ttcaaaagtg gttggagctt
     2821 tgatgtcaaa ccaatctttt ctggtgaatg ggtcaacgac cttctttttt aatcctttct
     2881 ttcctttgga caatctcttg tttttaccga cagccatgtt gtacttgagt tggatctacg
     2941 cgaaatttga aatataaata acgttcttaa tactaacata actataaaaa aataaatagg
     3001 gacgctagca tatgtcgaca agaaagagat cgatggacaa tgtgtatatt aagtgagatg
     3061 gatcggctta cgggtcagat gtaagtaacg cattatcaat ctcaatcatt aaatcggccc
     3121 aattgttttt agttagtgct tgctgaataa cttcttcgtc tgaatcaata tcggaatcac
     3181 tatcactagg ggaggtgtca attgtagacg ttatatccgg tgctggcaac tgtaaatgag
     3241 cgtcagaaat ggcaacaacc catttttcca ctactgtgct agatgtagat tgaatcaatg
     3301 tcgataaccc atcatctttt tgacgcactt gtaggatgga cgatccacga gaggagattt
     3361 ggatattctg catagggacg aatacagggt agtcaacact attccatata accatgtagt
     3421 tttcgaataa ataaacatac cttgtttgga aatagtccag tggctccaca caaacagata
     3481 gctcgtctgc aactcgcaaa tttcccaaca gaaccaatct tgacaagttg attttaggac
     3541 acagatctaa caaatatttg attagttggt ttctaaccgt ttcaacctca tatgcgtcca
     3601 cttctgtggt ggttgaaaca gttgggtctg gtgaaggaga tctggtaggt atatcgctgt
     3661 tttgtaaggt tgtctttaaa gcgttaatgt tgacactgtt ggggtgtgga ggtgctggtc
     3721 tcttgggtat taacgacaac tttctagcag aaaccaagtg ccagttgttt acatcgacaa
     3781 ctacgtttct ggtgccactg caatttaaac cacgacaggt tttggcaaac gttattgtct
     3841 tgccatggat tcttgcgtgt ggtatatctt ccttaaaata agctttgaaa cattcactgt
     3901 gggtggagtc accacaattt aactttagga ttcgttctga tgcaaataca gtagaaagta
     3961 gttctccaca gatgaaacat gtttcgtgat ttgctagtga cttgttgaga atattggcaa
     4021 aggaaaggtt tagtccggga tttatggttt gaaatgggtc tctggaaggg attttcgctg
     4081 gtcgtggact gacacatggc acacttggag gtgttgtatt gggtgtgatc gtcacactgc
     4141 ctggtggggt ggtagggcta gtgggcttct ttggagattt taaaaagtct ttccagctct
     4201 taggcgtaga ttgtagcatt gcctggtggg taatattaac agattgacga tggatgggat
     4261 ttttttggtg gttttatagt cgatgtttca gatttaggct ttccccgtca agctctaaat
     4321 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     4381 gattagggtg atggttcacg ggatatatcg atatatacgc gtatataagc tttttgaatg
     4441 attatatttt tttaatatta atatcgagat aatgtttcta agaaagaaag aaaaccagga
     4501 gtgaaaatta gaaaaggaaa ggaaaggaaa aaaagaaaaa tctgaaaata tataaaaaaa
     4561 aattgtttcg ttggcaataa atcctggtga gaacagcgac cgaaagcaaa taagaacaaa
     4621 atataagtgt attacgttga acaactaatt aacgtgtgtg tatggatctt tttttctttt
     4681 ttctctttaa ccgactataa acaacaaaca tttttgggca gtgcacacac actacttaat
     4741 atacacagca taaattacac gattagaaaa caaattagct tattaaaata acctaatcaa
     4801 accgaatatt ttatggtatt acgagtaaac tatataatat aaatagcaca cacccacaac
     4861 aacaacaaag gaaaactaaa aattttttct ttttgaaagg gccgttttct ttattattct
     4921 ctagttttga cgctgaaata ttccttgtac tgttgcaaat ttgtatttgg tctgccttat
     4981 cttcttttct acacttttta cttatgagct ctataattca cagataagtt tcaggtttgc
     5041 cagatatact tgcttcattg gttactaaac tctctgtaaa caattgactt gcttatgtaa
     5101 gaggctactt ttctagtttt gcagtcattc atttaaaaac aattgatcct gaaggctcta
     5161 aagacatagt aggtttacct tgagtgtata tttccgtggc atgtttaaag gccccttggc
     5221 cataggatat tggtggcggc acagtaagca ttaaaattgt tgtttcgcgg aattacaaca
     5281 tttcttcttt tacataataa tgtcactcct ttttggttgg catagccgag atcttttttt
     5341 aacacagact ttaagctgtt ttttgccacc tgagaacagt ttctattctg gtccaaaaga
     5401 aatattagac taagtttatt ataaacgagg ctggtgatct taatagctcc tcgagggggg
     5461 gcccggtacc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat
     5521 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     5581 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     5641 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     5701 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     5761 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     5821 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     5881 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     5941 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     6001 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     6061 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     6121 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     6181 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     6241 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     6301 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     6361 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     6421 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     6481 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     6541 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     6601 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     6661 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     6721 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     6781 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     6841 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     6901 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     6961 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     7021 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     7081 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     7141 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     7201 tgccatccgt aagatgcttt tctgttgcgc aacgttgttg ccattgctac aggcatcgtg
     7261 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     7321 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     7381 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     7441 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     7501 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     7561 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     7621 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     7681 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtg
//
