pCS4-BASK vector (V008139)

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LOCUS       V008139                10365 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008139
VERSION     V008139
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10365)
  AUTHORS   Thomson JG, Cook M, Guttman M, Smith J, Thilmony R.
  TITLE     Novel sulI binary vectors enable an inexpensive foliar selection
            method in Arabidopsis
  JOURNAL   BMC Res Notes 4 (1), 44 (2011)
   PUBMED   21366919
REFERENCE   2  (bases 1 to 10365)
  AUTHORS   Thomson JG, Cook M, Guttman M, Smith J, Thilmony R.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2010) Crop Improvement and Utilization Research
            Unit, USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA
REFERENCE   3  (bases 1 to 10365)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10365)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res
            Notes"; date: "2011"; volume: "4"; issue: "1"; pages: "44"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-NOV-2010) Crop Improvement and Utilization Research Unit,
            USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10365
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     regulatory      complement(145..304)
                     /label="CaMV35S polyA terminator"
                     /note="CaMV35S polyA terminator"
                     /regulatory_class="terminator"
     CDS             complement(362..1198)
                     /gene="sulI"
                     /label="Dihydropteroate synthase type-1"
                     /note="Dihydropteroate synthase type-1 from Escherichia
                     coli. Accession#: P0C002"
     promoter        complement(1307..1978)
                     /label="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     gene            2078..3671
                     /gene="Ubi"
                     /label="Ubi"
                     /note="ubiquitin"
     regulatory      2078..2866
                     /gene="Ubi"
                     /note="derived from Solanum bulbocastanum 409S; bul409S"
                     /regulatory_class="promoter"
     5'UTR           2867..3443
                     /gene="Ubi"
     intron          2910..3443
                     /gene="Ubi"
                     /note="bul409S Ubiquitin 5' intron"
     misc_feature    3444..3671
                     /gene="Ubi"
                     /note="ubitquitin monomer; designed for an N-terminal
                     translational fusion with genes of interest for
                     constitutive overexpression in transgenic plants; derived
                     from Solanum bulbocastanum 409S ubiquitin monomer"
     terminator      3753..4005
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4087..4111
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5412..6038
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             6475..7539
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      7608..7802
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    8146..8286
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8472..9060)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9150..9941)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"