LOCUS       V008139                10365 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008139
VERSION     V008139
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10365)
  AUTHORS   Thomson JG, Cook M, Guttman M, Smith J, Thilmony R.
  TITLE     Novel sulI binary vectors enable an inexpensive foliar selection
            method in Arabidopsis
  JOURNAL   BMC Res Notes 4 (1), 44 (2011)
   PUBMED   21366919
REFERENCE   2  (bases 1 to 10365)
  AUTHORS   Thomson JG, Cook M, Guttman M, Smith J, Thilmony R.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2010) Crop Improvement and Utilization Research
            Unit, USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA
REFERENCE   3  (bases 1 to 10365)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10365)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res
            Notes"; date: "2011"; volume: "4"; issue: "1"; pages: "44"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-NOV-2010) Crop Improvement and Utilization Research Unit,
            USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10365
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     regulatory      complement(145..304)
                     /label="CaMV35S polyA terminator"
                     /note="CaMV35S polyA terminator"
                     /regulatory_class="terminator"
     CDS             complement(362..1198)
                     /gene="sulI"
                     /label="Dihydropteroate synthase type-1"
                     /note="Dihydropteroate synthase type-1 from Escherichia
                     coli. Accession#: P0C002"
     promoter        complement(1307..1978)
                     /label="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     gene            2078..3671
                     /gene="Ubi"
                     /label="Ubi"
                     /note="ubiquitin"
     regulatory      2078..2866
                     /gene="Ubi"
                     /note="derived from Solanum bulbocastanum 409S; bul409S"
                     /regulatory_class="promoter"
     5'UTR           2867..3443
                     /gene="Ubi"
     intron          2910..3443
                     /gene="Ubi"
                     /note="bul409S Ubiquitin 5' intron"
     misc_feature    3444..3671
                     /gene="Ubi"
                     /note="ubitquitin monomer; designed for an N-terminal
                     translational fusion with genes of interest for
                     constitutive overexpression in transgenic plants; derived
                     from Solanum bulbocastanum 409S ubiquitin monomer"
     terminator      3753..4005
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4087..4111
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5412..6038
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             6475..7539
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      7608..7802
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    8146..8286
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8472..9060)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9150..9941)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcct aggccaccat gttgggcccg
      121 gccaagcttg catgcctgca ggtcactgga ttttggtttt aggaattaga aattttattg
      181 atagaagtat tttacaaata caaatacata ctaagggttt cttatatgct caacacatga
      241 gcgaaaccct ataagaaccc taattccctt atctgggaac tactcacaca ttattctgga
      301 gaaaaataga gagagataga tttgtagaga gagactggtg atttttgcgg actctagact
      361 aggcatgatc taaccctcgg tctctggcgt cgcgactgcg aaatttcgcg agggtttccg
      421 agatggtgat tgcgcttcgc agatctccag gcgcgtgggt gcggacgtag tcagcgccat
      481 tgccgatcgc gtgaagttcc gccgcaaggc tcgctggacc cagatccttt acaggaaggc
      541 caacggtggc gcccaagaag gatttccgcg acaccgagac caatagcgga agccccaacg
      601 ccgacttcag cttttgaagg ttcgacagca cgtgcagcga tgtttccggt gcggggctca
      661 agaaaaatcc catccccgga tcgaggatta gccggtcggc agcgaccccg ctccgtcgca
      721 aggcggaaac ccgcgcctcg aagaaccgca caatctcgtc gagcgcgtct tcgggtcgaa
      781 ggtgaccggt gcgggtggcg atgccatccc gctgcgctga gtgcataacc accagcctgc
      841 agtccgcctc agcaatatcg ggatagagcg cagggtcagg aaatccttgg atatcgttca
      901 ggtagcccac gccgcgcttg agcgcatagc gctgggtttc cggttggaag ctgtcgattg
      961 aaacacggtg catctgatcg gacagggcgt ctaagagcgg cgcaatacgt ctgatctcat
     1021 cggccggcga tacaggcctc gcgtccggat ggctggcggc cggtccgaca tccacgacgt
     1081 ctgatccgac tcgcagcatt tcgatcgccg cggtgacagc gccggcgggg tctagccgcc
     1141 ggctctcatc gaagaaggag tcctcggtga gattcagaat gccgaacacc gtcaccatgc
     1201 actttactct tccaccattg cttgtaatgg aagtaatgtc agtgttgacc ttcttaactg
     1261 ggaatccagt catggatttg aggccgccga atgggcccat ctcgaggtcc tctccaaatg
     1321 aaatgaactt ccttatatag aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc
     1381 ccttacgtca gtggagatat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc
     1441 ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga
     1501 ggcatcttca acgatggcct ttcctttatc gcaatgatgg catttgtagg agccaccttc
     1561 cttttccact atcttcacaa taaagtgaca gatagctggg caatggaatc cgaggaggtt
     1621 tccggatatt accctttgtt gaaaagtctc aattgccctt tggtcttctg agactgtatc
     1681 tttgatattt ttggagtaga caagtgtgtc gtgctccacc atgttatcac atcaatccac
     1741 ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg
     1801 tccatctttg ggaccactgt cggcagaggc atcttcaacg atggcctttc ctttatcgca
     1861 atgatggcat ttgtaggagc caccttcctt ttccactatc ttcacaataa agtgacagat
     1921 agctgggcaa tggaatccga ggaggtttcc ggatattacc ctttgttgaa aagtctcaat
     1981 tgccctttgg tcttctgaga ctgtatcttt gatatttttg gagtagacaa gtgtgtcgtg
     2041 ctccaccatg ttgacctgca ggcatgcaag cttaagctac ttacctctta tataaaaaag
     2101 aaaaagtgtt tctaatatat actcaattta aataaaatat tttcaatcaa atttagataa
     2161 caaatactta tcaatatgag gtcaataaca ataaaaaaat aatgtaaaaa aaaaggagca
     2221 atacataata taagaaaaaa gattaaagtg cgattatcaa cgagtattat accctaattt
     2281 gctaatattt aaactcttat atttaaggtt atgttcacaa tatacttaaa aagcgctata
     2341 ttagagcata tattaattaa taaaaaagaa aatgctaaat gatcaaaaaa attagataga
     2401 aaattaagaa aattataata tttttttatt ttaaaataaa ttgatatatt ctttattttt
     2461 tagttaaaat gtattaaagt taaaagaata aaaatatttt aaaaaataaa ataacataaa
     2521 taaaatatca ttctaattaa attcagacca aattttttcc ccagattttg gccaatacct
     2581 aaaataaaat taagttattt ttagtatatt tttttacatt gacctacatt tttctagttt
     2641 tttctaaagg agcgtgtaag cgtcaacctc attctcctaa ttttccccac cacataaata
     2701 aaaagaaacg gtagcttttg cgtgttgttt tgctacacta cacctcatta ttacacgtgt
     2761 catcatataa ttggctaacc ctatgaggcg gtttcgtcta gagtcggcca tgccatctat
     2821 aaaaggaacc tttctgcacc tcattttttc atcttctatc tgacttctat tataatttct
     2881 ctcaattgcc tttaaatttc tctttcaagg ttagaaatct tctctatttt ttggtttttg
     2941 tctgtttaga ttctcgaatt agctaagcag gtgctgttaa agccctaaaa tttgagtttt
     3001 ttttccgttg ttttgatgaa aaagccccta atttgagttt ttttccgtcg atttgatgcc
     3061 aaaggtttaa aattagagtt ttttcgtcgg tttgattcta aaggcccaaa atgtggggtt
     3121 ttccgggtga tttgatgata atgccctaga atttgagttt ttttatggtg gtttgatgaa
     3181 aaaggtcttg aatttgattt tttttttccg gttgatttga tgaaaaagcc ctagaatttg
     3241 tgatttttcg tcggtttgat tctaaagccc taaaatttga ggttttccgg ttgttttgat
     3301 gaaaaagccc taaaatttga gttttttccc cgtgttttag attgtttggt tttaattctc
     3361 gaatcagtta atcagggagt gtgaaaagcc ctataatttg agtttttttc gttgttccga
     3421 ttgttgtttt tatgactttg cagatgcaga tctttgtgaa aactctcacc ggaaagacca
     3481 tcaccctaga ggtggaacgt tctgatacaa tcgacaacgt taaggctgag attcaggata
     3541 aggaaggaat tcccccggat cagcaaaggc ttatcttcgc cggaaagcag ttggaggacg
     3601 gacgtactct agctgattac aacatccaga aggagtctac cctccatttg gttctccgtc
     3661 tacgtggtgg tggaggatcc atggggcgcg ccactagtgg taccgccccg tccggtcctg
     3721 cccgtcaccg agatctgatc cgtcgacctg cagatcgttc aaacatttgg caataaagtt
     3781 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
     3841 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta
     3901 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa
     3961 actaggataa attatcgcgc gcggtgtcat ctatgttact agatccgatg ataagctgtc
     4021 aaacatgaga attcacgcgc gcggtgtcat ctatgttact agatcgggaa ttaaactatc
     4081 agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa
     4141 tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca
     4201 accacagggt tcccctcggg atcaaagtac tttgatccaa cccctccgct gctatagtgc
     4261 agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta
     4321 agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag
     4381 tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc
     4441 cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca
     4501 acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac
     4561 caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt
     4621 gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga
     4681 gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac
     4741 cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc
     4801 cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt
     4861 tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca
     4921 ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta
     4981 ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt
     5041 ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga
     5101 ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag
     5161 atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc
     5221 gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctggcggc ctggccggcc
     5281 agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa
     5341 aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc
     5401 aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga
     5461 ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg
     5521 attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa
     5581 ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg
     5641 acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg
     5701 cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg
     5761 acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct
     5821 ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg
     5881 ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca
     5941 ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg
     6001 tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa
     6061 aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc
     6121 tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc
     6181 ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga
     6241 gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta
     6301 gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg
     6361 ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg
     6421 tctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgacggtcgc
     6481 aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg
     6541 aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg
     6601 tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg
     6661 ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc
     6721 tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg
     6781 aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag
     6841 gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg
     6901 gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc
     6961 cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga
     7021 aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg
     7081 cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg
     7141 attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag
     7201 ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt
     7261 caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc
     7321 cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc
     7381 agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac
     7441 ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc
     7501 taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta
     7561 gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg
     7621 tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag
     7681 ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat
     7741 ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca
     7801 taactgtctg gccagcgcac agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg
     7861 cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg
     7921 gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac
     7981 cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
     8041 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     8101 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
     8161 gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta
     8221 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     8281 atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     8341 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     8401 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8461 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8521 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8581 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8641 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8701 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8761 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8821 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8881 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     8941 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     9001 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     9061 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     9121 gggattttgg tcatgcattc taggtactaa aacaattcat ccagtaaaat ataatatttt
     9181 attttctccc aatcaggctt gatccccagt aagtcaaaaa atagctcgac atactgttct
     9241 tccccgatat cctccctgat cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc
     9301 ctgccgcttc tcccaagatc aataaagcca cttactttgc catctttcac aaagatgttg
     9361 ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc cgtctttaaa
     9421 aaatcataca gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc
     9481 acatcggcca gatcgttatt cagtaagtaa tccaattcgg ctaagcggct gtctaagcta
     9541 ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac
     9601 agctcgataa tcttttcagg gctttgttca tcttcatact cttccgagca aaggacgcca
     9661 tcggcctcac tcatgagcag attgctccag ccatcatgcc gttcaaagtg caggaccttt
     9721 ggaacaggca gctttccttc cagccatagc atcatgtcct tttcccgttc cacatcatag
     9781 gtggtccctt tataccggct gtccgtcatt tttaaatata ggttttcatt ttctcccacc
     9841 agcttatata ccttagcagg agacattcct tccgtatctt ttacgcagcg gtatttttcg
     9901 atcagttttt tcaattccgg tgatattctc attttagcca tttattattt ccttcctctt
     9961 ttctacagta tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac
    10021 tgttccttgc attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa
    10081 agttggcgta taacatagta tcgacggagc cgattttgaa accgcggtga tcacaggcag
    10141 caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa
    10201 acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg
    10261 ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg
    10321 tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctgg
//
