pBD62 vector (V009239)

LOCUS       40924_6157        3226 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Integrative expression vector pBD62, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Das B, Kumari R, Pant A, Sen Gupta S, Saxena S, Nair GB.
  TITLE     CTXPhi Derived Novel Broad Host Range Integrative Expression Vector 
            For Functional Study
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Das B, Kumari R, Pant A, Sen Gupta S, Saxena S, Nair GB.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-2014) Centre for Human Microbial Ecology, 
            Translational Health Science and Technology Institute, 496 Udyog 
            Vihar Phase III, Gurgaon, Haryana 122016, India
REFERENCE   3  (bases 1 to 3226)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3226)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-APR-2014) Centre for Human Microbial Ecology, Translational 
            Health Science and Technology Institute, 496 Udyog Vihar Phase III, 
            Gurgaon, Haryana 122016, India"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        12..30
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     oriT            184..293
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(402..1277)
                     /label=araC
                     /note="L-arabinose regulatory protein"
     promoter        1304..1588
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     regulatory      1609..1614
                     /regulatory_class="ribosome_binding_site"
     primer_bind     complement(1633..1649)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(1686..1704)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(1719..2107)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(2285..2656)
                     /label=BleoR
                     /note="antibiotic-binding protein"
     promoter        complement(2675..2722)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     promoter        complement(2749..2851)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     misc_recomb     3036..3187
                     /label=attP
                     /note="attP"