LOCUS       40924_6157        3226 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Integrative expression vector pBD62, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Das B, Kumari R, Pant A, Sen Gupta S, Saxena S, Nair GB.
  TITLE     CTXPhi Derived Novel Broad Host Range Integrative Expression Vector 
            For Functional Study
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Das B, Kumari R, Pant A, Sen Gupta S, Saxena S, Nair GB.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-2014) Centre for Human Microbial Ecology, 
            Translational Health Science and Technology Institute, 496 Udyog 
            Vihar Phase III, Gurgaon, Haryana 122016, India
REFERENCE   3  (bases 1 to 3226)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3226)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-APR-2014) Centre for Human Microbial Ecology, Translational 
            Health Science and Technology Institute, 496 Udyog Vihar Phase III, 
            Gurgaon, Haryana 122016, India"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        12..30
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     oriT            184..293
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(402..1277)
                     /label=araC
                     /note="L-arabinose regulatory protein"
     promoter        1304..1588
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     regulatory      1609..1614
                     /regulatory_class="ribosome_binding_site"
     primer_bind     complement(1633..1649)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(1686..1704)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(1719..2107)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(2285..2656)
                     /label=BleoR
                     /note="antibiotic-binding protein"
     promoter        complement(2675..2722)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     promoter        complement(2749..2851)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     misc_recomb     3036..3187
                     /label=attP
                     /note="attP"
ORIGIN
        1 tcgagcgcgc gtaatacgac tcactatagg gcgaattggg taccagcgct tttccgctgc
       61 ataaccctgc ttcggggtca ttatagcgat tttttcggta tatccatcct ttttcgcacg
      121 atatacagga ttttgccaaa gggttcgtgt agactttcct tggtgtatcc aacggcgtca
      181 gccgggcagg ataggtgaag taggcccacc cgcgagcggg tgttccttct tcactgtccc
      241 ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg cgaggctggc cggcgtcgac
      301 ggtatcgatg cataatgtgc ctgtcaaatg gacgaagcag ggattctgca aaccctatgc
      361 tactccgtca agccgtcaat tgtctgattc gttaccaatt atgacaactt gacggctaca
      421 tcattcactt tttcttcaca accggcacgg aactcgctcg ggctggcccc ggtgcatttt
      481 ttaaataccc gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg
      541 ataggcatcc gggtggtgct caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc
      601 cagcttaaga cgctaatccc taactgctgg cggaaaagat gtgacagacg cgacggcgac
      661 aagcaaacat gctgtgcgac gctggcgata tcaaaattgc tgtctgccag gtgatcgctg
      721 atgtactgac aagcctcgcg tacccgatta tccatcggtg gatggagcga ctcgttaatc
      781 gcttccatgc gccgcagtaa caattgctca agcagattta tcgccagcag ctccgaatag
      841 cgcccttccc cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg
      901 tgcgcttcat ccgggcgaaa gaaccccgta ttggcaaata ttgacggcca gttaagccat
      961 tcatgccagt aggcgcgcgg acgaaagtaa acccactggt gataccattc gcgagcctcc
     1021 ggatgacgac cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc acccggtcgg
     1081 caaacaaatt ctcgtccctg atttttcacc accccctgac cgcgaatggt gagattgaga
     1141 atataacctt tcattcccag cggtcggtcg ataaaaaaat cgagataacc gttggcctca
     1201 atcggcgtta aacccgccac cagatgggca ttaaacgagt atcccggcag caggggatca
     1261 ttttgcgctt cagccatact tttcatactc ccgccattca gagaagaaac caattgtcca
     1321 tattgcatca gacattgccg tcactgcgtc ttttactggc tcttctcgct aaccaaaccg
     1381 gtaaccccgc ttattaaaag cattctgtaa caaagcggga ccaaagccat gacaaaaacg
     1441 cgtaacaaaa gtgtctataa tcacggcaga aaagtccaca ttgattattt gcacggcgtc
     1501 acactttgct atgccatagc atttttatcc ataagattag cggatcctac ctgacgcttt
     1561 ttatcgcaac tctctactgt ttctccatac ccgttttttt gggctagcag gaggaattcc
     1621 tgcagcccgg gggatccact agttctagag cggccgccac cgcggtggag ctccagcttt
     1681 tgttcccttt agtgagggtt aattgcgcgc aattcccatg tcagccgtta agtgttcctg
     1741 tgtcactcaa aattgctttg agaggctcta agggcttctc agtgcgttac atccctggct
     1801 tgttgtccac aaccgttaaa ccttaaaagc tttaaaagcc ttatatattc ttttttttct
     1861 tataaaactt aaaaccttag aggctattta agttgctgat ttatattaat tttattgttc
     1921 aaacatgaga gcttagtacg tgaaacatga gagcttagta cgttagccat gagagcttag
     1981 tacgttagcc atgagggttt agttcgttaa acatgagagc ttagtacgtt aaacatgaga
     2041 gcttagtacg tgaaacatga gagcttagta cgtactatca acaggttgaa ctgctgatct
     2101 tcagatcctc tacgccggac gcatcgtggc cggatcagat ctgatatcgt cgcagaccaa
     2161 aacgatctca agaagatcat cttattaatc agataaaata tttctaggca ccaataactg
     2221 ccttaaaaaa attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcaatgcat
     2281 ctcagtcctg ctcctcggcc acgaagtgca cgcagttgcc ggccgggtcg cgcagggcga
     2341 actcccgccc ccacggctgc tcgccgatct cggtcatggc cggcccggag gcgtcccgga
     2401 agttcgtgga cacgacctcc gaccactcgg cgtacagctc gtccaggccg cgcacccaca
     2461 cccaggccag ggtgttgtcc ggcaccacct ggtcctggac cgcgctgatg aacagggtca
     2521 cgtcgtcccg gaccacaccg gcgaagtcgt cctccacgaa gtcccgggag aacccgagcc
     2581 ggtcggtcca gaactcgacc gctccggcga cgtcgcgcgc ggtgagcacc ggaacggcac
     2641 tggtcaactt ggccatggtt tagttcctca ccttgtcgta ttatactatg ccgatatact
     2701 atgccgatga ttaattgtca acacgtgctc gagctagcga tcgaccattt tagcttcctt
     2761 agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg
     2821 gtgaaagttg gaacctctta cgtgccgatc aacgtctcat tttcgccaaa agttggccca
     2881 gggcttcccg gtatcaacag ggacaccagg atttatttat tctgcgaagt gatcttccgt
     2941 cacaggctcg agaagctgat gactgcggcg gacggctgga atcagaataa atcgaattat
     3001 gtcatttttg acataccgaa atgacatacc gccttaatgc gtattatacg ccattatgtt
     3061 acgggctcac tcttgcagaa cgattcagca agctgcccac gttctgctgc aagtgaggcc
     3121 gtcagtgctg gcgcaatggt actgacgact gcccctcaac ataatgccgc gccacataat
     3181 gcgcactagg aacattttgt ctctaggttt agtacggtgg ctttgc
//
