pBAKA vector (V009286)

LOCUS       V009286                 4488 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009286
VERSION     V009286
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Barrett AR, Kang Y, Inamasu KS, Son MS, Vukovich JM, Hoang TT.
  TITLE     Genetic tools for allelic replacement in Burkholderia species
  JOURNAL   Appl. Environ. Microbiol. 74 (14), 4498-4508 (2008)
   PUBMED   18502918
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Barrett AR, Kang Y, Son MS, Vukovich JM, Hoang TT.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2007) Microbiology, University of Hawaii Manoa,
            2538 McCarthy Mall Snyder Hall, Rm. 310, Honolulu, HI 96822, USA
REFERENCE   3  (bases 1 to 4488)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4488)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2008"; volume: "74"; issue: "14";
            pages: "4498-4508"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (05-NOV-2007) Microbiology, University of Hawaii Manoa, 2538
            McCarthy Mall Snyder Hall, Rm. 310, Honolulu, HI 96822, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4488
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            complement(20..129)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     terminator      complement(550..577)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(669..755)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     1105..1121
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    complement(1125..1181)
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1191..1207)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1215..1231)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1239..1269)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1284..1305)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1593..2181)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2266..3381)
                     /codon_start=1
                     /gene="asd"
                     /product="aspartate semialdehyde dehydrogenase"
                     /label="asd"
                     /note="Asd"
                     /protein_id="ABY75257.1"
                     /translation="MKRVGLIGWRGMVGSVLIQRMLEERDFDLIEPVFFTTSNVGAQAP
                     EVDKDIAPLKDAYSIDELKTLDVILTCQGGDYTSEVFPKLREAGWQGYWIDAASSLRME
                     DDAVIVLDPVNRKVIDQALDAGTRNYIGGNCTVSLMLMALGGLFDAGLVEWMSAMTYQA
                     ASGAGAQNMRDLLKQMGAAHASVADDLANPASAILDIDRKVAETLRSEAFPTEHFGAPL
                     GGSLIPWIDKELSQRRQSREEWKAQAETNKILARFKNPIPVDGICVRVGAMRCHSQALT
                     IKLNKDVPLTDIEGLIRQHNPWVKLVPNHREVSVRELTPAAVTGTLSVPVGRLRKLNMV
                     SQYLGAFTVGDQLLWGAAEPLRRMLRILLER"
     gene            complement(2266..3381)
                     /gene="asd"
                     /label="asd"
     CDS             complement(3398..4408)
                     /gene="pheS"
                     /label="Phenylalanine--tRNA ligase alpha subunit"
                     /note="Phenylalanine--tRNA ligase alpha subunit from
                     Burkholderia pseudomallei (strain 1710b). Accession#:
                     Q3JT08"
     regulatory      complement(4444..4486)
                     /regulatory_class="promoter"