LOCUS       V009286                 4488 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009286
VERSION     V009286
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Barrett AR, Kang Y, Inamasu KS, Son MS, Vukovich JM, Hoang TT.
  TITLE     Genetic tools for allelic replacement in Burkholderia species
  JOURNAL   Appl. Environ. Microbiol. 74 (14), 4498-4508 (2008)
   PUBMED   18502918
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Barrett AR, Kang Y, Son MS, Vukovich JM, Hoang TT.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2007) Microbiology, University of Hawaii Manoa,
            2538 McCarthy Mall Snyder Hall, Rm. 310, Honolulu, HI 96822, USA
REFERENCE   3  (bases 1 to 4488)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4488)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2008"; volume: "74"; issue: "14";
            pages: "4498-4508"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (05-NOV-2007) Microbiology, University of Hawaii Manoa, 2538
            McCarthy Mall Snyder Hall, Rm. 310, Honolulu, HI 96822, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4488
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            complement(20..129)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     terminator      complement(550..577)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(669..755)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     1105..1121
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    complement(1125..1181)
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1191..1207)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1215..1231)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1239..1269)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1284..1305)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1593..2181)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2266..3381)
                     /codon_start=1
                     /gene="asd"
                     /product="aspartate semialdehyde dehydrogenase"
                     /label="asd"
                     /note="Asd"
                     /protein_id="ABY75257.1"
                     /translation="MKRVGLIGWRGMVGSVLIQRMLEERDFDLIEPVFFTTSNVGAQAP
                     EVDKDIAPLKDAYSIDELKTLDVILTCQGGDYTSEVFPKLREAGWQGYWIDAASSLRME
                     DDAVIVLDPVNRKVIDQALDAGTRNYIGGNCTVSLMLMALGGLFDAGLVEWMSAMTYQA
                     ASGAGAQNMRDLLKQMGAAHASVADDLANPASAILDIDRKVAETLRSEAFPTEHFGAPL
                     GGSLIPWIDKELSQRRQSREEWKAQAETNKILARFKNPIPVDGICVRVGAMRCHSQALT
                     IKLNKDVPLTDIEGLIRQHNPWVKLVPNHREVSVRELTPAAVTGTLSVPVGRLRKLNMV
                     SQYLGAFTVGDQLLWGAAEPLRRMLRILLER"
     gene            complement(2266..3381)
                     /gene="asd"
                     /label="asd"
     CDS             complement(3398..4408)
                     /gene="pheS"
                     /label="Phenylalanine--tRNA ligase alpha subunit"
                     /note="Phenylalanine--tRNA ligase alpha subunit from
                     Burkholderia pseudomallei (strain 1710b). Accession#:
                     Q3JT08"
     regulatory      complement(4444..4486)
                     /regulatory_class="promoter"
ORIGIN
        1 actgttacgc cggcggtagc cggccagcct cgcagagcag gattcccgtt gagcaccgcc
       61 aggtgcgaat aagggacagt gaagaaggaa cacccgctcg cgggtgggcc tacttcacct
      121 atcctgcccg gctgacgccg ttggatacac caaggaaagt ctacacgaac cctttggcaa
      181 aatcctgtat atcgtgcgaa aaaggatgga tataccgaaa aaatcgctat aatgaccccg
      241 aagcagggtt atgcagcgga aaagcgctgc ttccctgctg ttttgtggaa tatctaccga
      301 ctggaaacag gcaaatgcag gaaattactg aactgagggg acaggcgaga gacgatgcca
      361 aagagctaca ccgacgagct ggccgagtgg gttgaatccc gcgcggccaa gaagcgccgg
      421 cgtgatgagg ctgcggttgc gttcctggcg gtgagggcgg atgtcgatat gcgtaaggag
      481 aaaataccgc atcaggcgca tatttgaatg tatttagaaa aataaacaaa aagagtttgt
      541 agaaacgcaa aaaggccatc cgtcaggatg gccttctgct taatttgatg cctggcagtt
      601 tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcgcaacgtt caaatccgct
      661 cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag
      721 gcccagtctt tcgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc
      781 atggggagac cccacactac catcggcgct acggcgtttc acttctgagt tcggcatggg
      841 gtcaggtggg accaccgcgc tactgccgcc aggcaaattc tgttttatca gaccgcttct
      901 gcgttctgat ttaatctgta tcaggctgaa aatcttctct catccgccaa aacagccaag
      961 ctcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     1021 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     1081 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctt gcatgcctgc
     1141 aggtcgactc tagaggatcc ccgggtaccg agctcgaatt cgtaatcatg gtcatagctg
     1201 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     1261 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     1321 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     1381 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     1441 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     1501 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     1561 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     1621 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     1681 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     1741 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     1801 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     1861 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     1921 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     1981 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     2041 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     2101 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     2161 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     2221 tggaacgaaa actcacgtta agggattttg gtcatggatc tccgatcagc gctccagcag
     2281 gatgcgcaac atgcgccgca gcggctcggc agcgccccac agcaactggt cgccgacggt
     2341 gaaggcgccg agatactggg agaccatgtt caacttgcgc aggcgcccga ccggaacgct
     2401 cagggtgccg gtgacggcgg ccggggtcag ctcgcgcacg cttacctcgc ggtggttcgg
     2461 caccagcttg acccagggat tgtgctgtct gatcaggccc tcgatgtcgg tcagcggcac
     2521 atccttgttc aacttgatgg tcagcgcctg gctgtggcaa cgcatggcgc cgacgcgcac
     2581 gcagatacca tcgaccggga tcgggttctt gaagcgggca aggatcttgt tggtctccgc
     2641 ctgggccttc cactcttcgc ggctctgtct ccgttgggac agttccttgt cgatccaggg
     2701 gatcaggctg ccgcccagcg gcgcgccgaa atgttcggtg gggaaggctt cgctgcgcag
     2761 ggtctccgcc accttgcggt cgatgtcgag gatcgcgctg gccgggttgg ccaggtcatc
     2821 cgccaccgat gcatgggcgg cgcccatctg cttgagcaga tcacgcatgt tctgcgcgcc
     2881 ggcgccggag gcggcctggt aggtcatggc actcatccac tcgaccagac cggcgtcgaa
     2941 cagcccgccc agggccatca gcatcaggct gacggtgcag ttgccgccga tgtagttgcg
     3001 ggtgccggcg tccagagcct ggtcgatgac cttgcggttg accgggtcga ggacgatcac
     3061 cgcgtcgtct tccatgcgca gactggaggc ggcgtcgatc cagtagccct gccagccggc
     3121 ctcgcgcagc ttggggaata cttcgctggt gtagtcgccg ccctggcagg tcaggatcac
     3181 gtcgagggtc ttcagctcgt cgatgctgta ggcgtccttc agcggagcga tgtccttgtc
     3241 cacttccggg gcctgggcac cgacgttcga cgtggtgaag aacaccggct cgatcaggtc
     3301 gaagtcccgc tcttccagca ttcgctgtat gagtacggaa cccaccatcc cgcgccaccc
     3361 gatcagacct acacgcttca tcgcaactgg atctttaggc gaactggcgg aggaaccgga
     3421 ggtcattttc gaaaaagagc cggaggtcct ggacaccata ccggagcatg gtaaggcgtt
     3481 ccaacccgga accgaagccg aagccaatgt aacgctccgg gtcgaggccc atattccgaa
     3541 tgaccgtggg gtggacctga ccgctgccgc tgatttccaa ccacttacca gcattcttcc
     3601 catgttcaaa catcatatca atttccgcgc tgggttccgt gaaggggaag tagctcgggc
     3661 ggaaacggac caagatgtca tctcgttcga aaaatttctt gagaaagtcg gtgtaggcac
     3721 ccttgagatc ggcaaacgac acgttttcat caatccagag cccctccacc tgattaaaca
     3781 tggggctatg ggtggcgtcc gaatcgaccc gataggtccg accgggagca atcaccttaa
     3841 tcgggggtcg attcatgcgg gcgtaccgca cctgcatggg cgaggtatgg gtccggagga
     3901 ggaggggccg cccatcggcg tccttaccat ccacatagaa ggtgtcctgc atggaccgtg
     3961 cggggtggtt ttcgggcgag ttgagcgccg tgaaattata ccaatccgtc tcaatttccg
     4021 gtccatccgc gacatcgaag ccaatgctac ggaaaatctg ctccacacgt tcccaggttc
     4081 gcatgacggg gtgcagcgag ccggcgcccg ccccgcgacc ggggagggtc acatcaatgg
     4141 cttcagccgc caggcgctga ttgagaaggg catccgccaa ggcctgccgc cgggcattca
     4201 aggccgcctc cacctgctgc ttggccacgt taatccgggc accctcggtc ttccgggttt
     4261 ccgggtccag cttacccagc cccttcaaga gttccgtgag ggcaccgctc ttgcccagga
     4321 agcgggcctt ttcattttcc aaggtcgtaa tgtcggccgc gccctcaaag ctctgctggg
     4381 catccgccac gatctgatcg aggtccatat gcttgtcctt tcgaagctcg atccgattgt
     4441 cagacccggc atgatattcc gaaaatccca agcgagtcaa cagctagt
//
