pAMNPT-MCS vector (V009503)

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LOCUS       40924_4474        5694 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAMNPT-MCS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Myrach T, Witte CP.
  TITLE     Improvement of urea use efficiency in Arabidopsis thaliana
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5694)
  AUTHORS   Myrach T, Lipka V, Rademacher T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-FEB-2014) Department of Plant Biochemistry, Frei 
            Universitat Berlin, Dahlem Centre of Plant Sciences, 
            Koenigin-Luise-Str. 12-16, Berlin 14195, Germany
REFERENCE   3  (bases 1 to 5694)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5694)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-FEB-2014) Department of Plant Biochemistry, Frei Universitat 
            Berlin, Dahlem Centre of Plant Sciences, Koenigin-Luise-Str. 12-16, 
            Berlin 14195, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     3..19
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(53..69)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    352..376
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      488..1197
                     /label=oriV
                     /note="incP origin of replication"
     promoter        1271..1362
                     /label=AmpR promoter
     CDS             1363..2220
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2394..2982
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3290..3314
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     regulatory      complement(3372..3660)
                     /label=pAOcs
                     /note="pAOcs"
                     /regulatory_class="terminator"
     CDS             complement(3837..4628)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(4664..4845)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        5021..5694
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"