LOCUS       40924_4474        5694 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAMNPT-MCS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Myrach T, Witte CP.
  TITLE     Improvement of urea use efficiency in Arabidopsis thaliana
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5694)
  AUTHORS   Myrach T, Lipka V, Rademacher T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-FEB-2014) Department of Plant Biochemistry, Frei 
            Universitat Berlin, Dahlem Centre of Plant Sciences, 
            Koenigin-Luise-Str. 12-16, Berlin 14195, Germany
REFERENCE   3  (bases 1 to 5694)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5694)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-FEB-2014) Department of Plant Biochemistry, Frei Universitat 
            Berlin, Dahlem Centre of Plant Sciences, Koenigin-Luise-Str. 12-16, 
            Berlin 14195, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     3..19
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(53..69)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    352..376
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      488..1197
                     /label=oriV
                     /note="incP origin of replication"
     promoter        1271..1362
                     /label=AmpR promoter
     CDS             1363..2220
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2394..2982
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3290..3314
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     regulatory      complement(3372..3660)
                     /label=pAOcs
                     /note="pAOcs"
                     /regulatory_class="terminator"
     CDS             complement(3837..4628)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(4664..4845)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        5021..5694
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
ORIGIN
        1 ctcgaggtcg acggtatcga taagcttgat atcgaattcc tgcagcccgg gggatccact
       61 agttctagag tccgcaaaaa tcaccagtct ctctctacaa atctatctct ctctattttt
      121 ctccagaata atgtgtgagt agttcccaga taagggaatt agggttctta tagggtttcg
      181 ctcatgtgtt gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta
      241 tcaataaaat ttctaattcc taaaaccaaa atccagtgac cgggcggccg ccaccgcggt
      301 ggagggggat cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat
      361 atattggcgg gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag
      421 ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgta catcaccgac gagcaaggca
      481 agaccgagcg cctttccgac gctcaccggg ctggttgccc tcgccgctgg gctggcggcc
      541 gtctatggcc ctgcaaacgc gccagaaacg ccgtcgaagc cgtgtgcgag acaccggccg
      601 ccggcgttgt ggatacctcg cggaaaactt ggccctcact gacagatgag gggcggacgt
      661 tgacacttga ggggccgact cacccggcgc ggcgttgaca gatgaggggc aggctcgatt
      721 tcggccggcg acgtggagct ggccagcctc gcaaatcggc gaaaacgcct gattttacgc
      781 gagtttccca cagatgatgt ggacaagcct ggggataagt gccctgcggt attgacactt
      841 gaggggcgcg actactgaca gatgaggggc gcgatccttg acacttgagg ggcagagtgc
      901 tgacagatga ggggcgcacc tattgacatt tgaggggctg tccacaggca gaaaatccag
      961 catttgcaag ggtttccgcc cgtttttcgg ccaccgctaa cctgtctttt aacctgcttt
     1021 taaaccaata tttataaacc ttgtttttaa ccagggctgc gccctgtgcg cgtgaccgcg
     1081 cacgccgaag gggggtgccc ccccttctcg aaccctcccg gcccgctaac gcgggcctcc
     1141 catcccccca ggggctgcgc ccctcggccg cgaacggcct caccccaaaa atggcagcgc
     1201 tggcagtcct tgccattgcc gggatcgggg cagtaacggg atgggcgatc agcccgacaa
     1261 gctaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1321 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     1381 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     1441 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     1501 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     1561 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     1621 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     1681 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     1741 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     1801 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     1861 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     1921 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     1981 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     2041 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     2101 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     2161 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     2221 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     2281 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     2341 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2401 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2461 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2521 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     2581 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2641 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2701 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2761 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     2821 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     2881 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     2941 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3001 tttttacggt tcctggcctt ttgctggcct tttgctcaca tggactctag aggatcacag
     3061 gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc tccgcgagat catccgtgtt
     3121 tcaaacccgg cagcttagtt gccgttcttc cgaatagcat cggtaacatg agcaaagtct
     3181 gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg cctgtatcga
     3241 gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat
     3301 attgtggtgt aaacaaattg acgcttagac aacttaataa cacattgcgg acgtttttaa
     3361 tgtatcgaat tgtttgttat tgtggcgctc tatcatagat gtcgctataa acctattcag
     3421 cacaatatat tgttttcatt ttaatattgt acatataagt agtagggtac aatcagtaaa
     3481 ttgaacggag aatattattc ataaaaatac gatagtaacg ggtgatatat tcattagaat
     3541 gaaccgaaac cggcggtaag gatctgagct acacatgctc aggtttttta caacgtgcac
     3601 aacagaattg aaagcaaata tcatgcgatc ataggcgtct cgcatatctc attaaagcag
     3661 ggggtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc agccggcgtc
     3721 ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat cgaaatctcg
     3781 tgatggcagg ttgggcgtcg cttggtcggt catttcgaac cccagagtcc cgctcagaag
     3841 aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa
     3901 agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc
     3961 aacgctatgt cctgatagcg gtccgccaca cccagccggc cacagtcgat gaatccagaa
     4021 aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga
     4081 tcctcgccgt cgggcatgcg cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc
     4141 tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct
     4201 cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc
     4261 agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac
     4321 aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca
     4381 acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc
     4441 tcgtcctgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc
     4501 ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag
     4561 tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt
     4621 tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcag atccagatta tttggattga
     4681 gagtgaatat gagactctaa ttggataccg aggggaattt atggaacgtc agtggagcat
     4741 ttttgacaag aaatatttgc tagctgatag tgaccttagg cgacttttga acgcgcaata
     4801 atggtttctg acgtatgtgc ttagctcatt aaactccaga aacccgcggc tgagtggctc
     4861 cttcaacgtt gcggttctgt cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg
     4921 ggggtcataa cgtgactccc ttaattctcc gctcatgatc agattgtcgt ttcccgcctt
     4981 cggtttgggc gcgcccggtc tcagaagacc agagggctat tgagactttt caacaaaggg
     5041 taatatcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc atcgaaagga
     5101 cagtagaaaa ggaagatggc ttctacaaat gccatcattg cgataaagga aaggctatcg
     5161 ttcaagaatg cctctaccga cagtggtccc aaagatggac ccccacccac gaggaacatc
     5221 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgataacatg
     5281 gtggagcacg acactctcgt ctactccaag aatatcaaag atacagtctc agaagaccag
     5341 agggctattg agacttttca acaaagggta atatcgggaa acctcctcgg attccattgc
     5401 ccagctatct gtcacttcat cgaaaggaca gtagaaaagg aagatggctt ctacaaatgc
     5461 catcattgcg ataaaggaaa ggctatcgtt caagaatgcc tctaccgaca gtggtcccaa
     5521 agatggaccc ccacccacga ggaacatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     5581 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     5641 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggac
//
