pAM434 vector (V009516)

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LOCUS       V009516                 3410 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009516
VERSION     V009516
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Flannagan SE, Clewell DB.
  TITLE     Identification and characterization of genes encoding sex pheromone
            cAM373 activity in Enterococcus faecalis and Staphylococcus aureus
  JOURNAL   Mol. Microbiol. 44 (3), 803-817 (2002)
   PUBMED   11994160
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Flannagan SE, Clewell DB.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2001) School of Dentistry, The University of
            Michigan, 1011 N. University Ave., Ann Arbor, MI 48109-1078, USA
REFERENCE   3  (bases 1 to 3410)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3410)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol.
            Microbiol."; date: "2002"; volume: "44"; issue: "3"; pages:
            "803-817"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-OCT-2001) School of Dentistry, The University of Michigan, 1011
            N. University Ave., Ann Arbor, MI 48109-1078, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(40..58)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(79..95)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(103..119)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(127..157)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(172..193)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(481..1069)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     primer_bind     1474..1490
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        1500..1518
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1579..1595
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      2454..2459
                     /gene="erm(B)"
                     /regulatory_class="minus_35_signal"
     regulatory      2481..2486
                     /gene="erm(B)"
                     /regulatory_class="minus_10_signal"
     regulatory      2530..2534
                     /gene="erm(B)"
                     /regulatory_class="ribosome_binding_site"
     CDS             2542..3276
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"