LOCUS       V009516                 3410 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009516
VERSION     V009516
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Flannagan SE, Clewell DB.
  TITLE     Identification and characterization of genes encoding sex pheromone
            cAM373 activity in Enterococcus faecalis and Staphylococcus aureus
  JOURNAL   Mol. Microbiol. 44 (3), 803-817 (2002)
   PUBMED   11994160
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Flannagan SE, Clewell DB.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2001) School of Dentistry, The University of
            Michigan, 1011 N. University Ave., Ann Arbor, MI 48109-1078, USA
REFERENCE   3  (bases 1 to 3410)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3410)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol.
            Microbiol."; date: "2002"; volume: "44"; issue: "3"; pages:
            "803-817"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-OCT-2001) School of Dentistry, The University of Michigan, 1011
            N. University Ave., Ann Arbor, MI 48109-1078, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(40..58)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(79..95)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(103..119)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(127..157)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(172..193)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(481..1069)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     primer_bind     1474..1490
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        1500..1518
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1579..1595
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      2454..2459
                     /gene="erm(B)"
                     /regulatory_class="minus_35_signal"
     regulatory      2481..2486
                     /gene="erm(B)"
                     /regulatory_class="minus_10_signal"
     regulatory      2530..2534
                     /gene="erm(B)"
                     /regulatory_class="ribosome_binding_site"
     CDS             2542..3276
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
ORIGIN
        1 gtcgacctcg agggggggcc cggtacccag cttttgttcc ctttagtgag ggttaattgc
       61 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
      121 tccacacaac atacgagccg ggagcataaa gtgtaaagcc tggggtgcct aatgagtgag
      181 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
      241 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
      301 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
      361 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
      421 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
      481 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      541 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      601 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      661 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      721 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      781 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      841 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      901 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      961 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1021 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1081 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     1141 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     1201 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     1261 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     1321 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgatcggtgc
     1381 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     1441 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt
     1501 aatacgactc actatagggc gaattggagc tccaccgcgg tggcggccgc ttaattggcg
     1561 cgcctatata ggccggcctc tagaactagt ggatcccccg ggctgcagga attcgatatc
     1621 aagcttgttg taactgaaaa aggaaaatta ttgtgccagg cagttgaaag tcagcacctt
     1681 ttaacgagtg ctgaaatgac ggctaaatgg gaaacgtatt taaaaaaaat cggtaaaaga
     1741 gaaggcaatc aagagaactt tattacgaat atcaaaaaat tcattgttca tttactggaa
     1801 gctgtaccta acgatataga aaaactaaat ttttctgatt accaggaaca gaaagaaaaa
     1861 gaagcagaaa aaagtattgt aggaaaatgt cctaagtgtg gcaacaatat tgtattaaaa
     1921 aaatcgtttt atggttgttc aaattatcct gaatgtaagt ttactttagc tgaacatttt
     1981 agaaagaaaa aactcaccaa aacaaatgta aaagaattac tagagggaaa agaaaccctg
     2041 gtaaaaggaa tcaaaacgaa agatagaaag tcctacaatg ccgttgtaaa aatcggagaa
     2101 aagggatata ttgattttat atctttctca aaataaacat aaaagccctt taaagagggc
     2161 ttttatatat taatcacaaa tcacttatca caaatcacaa gtgatttgtg attgttgatg
     2221 ataaaataag aataagaaga aatagaaaga agtgagtgat tgtgggaaat ttaggcgcac
     2281 aaaaagaaaa acgaaatgat acaccaatca gtgcaaaaaa agatataatg ggagataaga
     2341 cggttcgtgt tcgtgctgac ttgcaccata tcataaaaat cgaaacagca aagaatggcg
     2401 gaaacgtaaa agaagttatg gaaataagac ttagaagcaa acttaagagt gtgttgatag
     2461 tgcagtatct taaaattttg tataatagga attgaagtta aattagatgc taaaaatttg
     2521 taattaagaa ggagtgatta catgaacaaa aatataaaat attctcaaaa ctttttaacg
     2581 agtgaaaaag tactcaacca aataataaaa caattgaatt taaaagaaac cgataccgtt
     2641 tacgaaattg gaacaggtaa agggcattta acgacgaaac tggctaaaat aagtaaacag
     2701 gtaacgtcta ttgaattaga cagtcatcta ttcaacttat cgtcagaaaa attaaaactg
     2761 aatactcgtg tcactttaat tcaccaagat attctacagt ttcaattccc taacaaacag
     2821 aggtataaaa ttgttgggag tattccttac catttaagca cacaaattat taaaaaagtg
     2881 gtttttgaaa gccatgcgtc tgacatctat ctgattgttg aagaaggatt ctacaagcgt
     2941 accttggata ttcaccgaac actagggttg ctcttgcaca ctcaagtctc gattcagcaa
     3001 ttgcttaagc tgccagcgga atgctttcat cctaaaccaa aagtaaacag tgtcttaata
     3061 aaacttaccc gccataccac agatgttcca gataaatatt ggaagctata tacgtacttt
     3121 gtttcaaaat gggtcaatcg agaatatcgt caactgttta ctaaaaatca gtttcatcaa
     3181 gcaatgaaac acgccaaagt aaacaattta agtaccgtta cttatgagca agtattgtct
     3241 atttttaata gttatctatt atttaacggg aggaaataat tctatgagtc gcttttgtaa
     3301 atttggaaag ttacacgtta ctaaagggaa tgtagataaa ttattaggta tactactgac
     3361 agcttccaag gagctaaaga ggtccctagc gcctacgggg aatttgtatc
//
