pAM2770 vector (V009518)

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LOCUS       40924_4379        8384 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector pAM2770, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8384)
  AUTHORS   Lee MH, Scherer M, Rigali S, Golden JW.
  TITLE     PlmA, a member of the GntR family, has plasmid maintenance functions
            in Anabaena sp. strain PCC 7120
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8384)
  AUTHORS   Lee MH.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAR-2003) Biology, Texas A
REFERENCE   3  (bases 1 to 8384)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8384)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-MAR-2003) Biology, Texas A"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             739..1530
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    2086..2228
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2414..3002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3103..3120)
                     /label=6xHis
                     /note="6xHis affinity tag"
     primer_bind     3377..3393
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(3397..3453)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     regulatory      complement(3467..3841)
                     /label=copper-inducible petE promoter
                     /note="copper-inducible petE promoter"
                     /regulatory_class="promoter"
     CDS             5291..6412
                     /codon_start=1
                     /gene="repA"
                     /product="RepA"
                     /label=repA
                     /note="required for replication"
                     /protein_id="AAO92601.1"
                     /translation="MISPESLNTNVSLTLLTRELQTEIILGTYTVRVHTRIGREPCARL
                     WYLCRALDKDGSGHLTLPLPVVQTFLDCSDKSVYRWLQDGKKIGAFRRYKIKAGMITVY
                     LGGMFQVCYNLNLKRWGDVAVVPLVQVLSDLRSLTTGIVTQSFQQKSRYAANRQLKPEY
                     RKLFGAPHPNELVKDTRQSSLKSPEGEVPCVLHISSSRIFVSKSFIHYGTSQKAVSCEL
                     GIHKRTVRRHQKQLGMNRRQLCQAKIEYNQLRHARNNDASEFWAFTGTKTDIGYQVMGD
                     AVVFSDGIAPGAKKRQPNTYQIDATEFDGRLFKVGDKVFMNRCNIYREQFTLTTMSAAR
                     RKYHFKLSQCHFSENRAGRVGNRFVIGCHSGEI"
     gene            5291..6412
                     /gene="repA"
                     /label=repA
     CDS             complement(7033..7584)
                     /codon_start=1
                     /gene="int/res"
                     /product="integrase/resolvase"
                     /label=int/res
                     /protein_id="AAO92602.1"
                     /translation="MKVNGNGRAKILTSDELRRLFSDGFTTPRDRVLFGICLFTGCRVS
                     EALALQTTDIKGETLTFRKSTTKGKLKTRVVDIQPGLAALMADYHPKPGTLFPGMRGVS
                     DRLTRYAADKILRDAAKRIGLEGISTHSFRRTALNQMSSAGIPLRHIQEISGHNDLGTL
                     QRYLEVTPEQRRKAVSVIGF"
     gene            complement(7033..7584)
                     /gene="int/res"
                     /label=int/res