LOCUS       40924_4379        8384 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector pAM2770, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8384)
  AUTHORS   Lee MH, Scherer M, Rigali S, Golden JW.
  TITLE     PlmA, a member of the GntR family, has plasmid maintenance functions
            in Anabaena sp. strain PCC 7120
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8384)
  AUTHORS   Lee MH.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAR-2003) Biology, Texas A
REFERENCE   3  (bases 1 to 8384)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8384)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-MAR-2003) Biology, Texas A"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             739..1530
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    2086..2228
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2414..3002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3103..3120)
                     /label=6xHis
                     /note="6xHis affinity tag"
     primer_bind     3377..3393
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(3397..3453)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     regulatory      complement(3467..3841)
                     /label=copper-inducible petE promoter
                     /note="copper-inducible petE promoter"
                     /regulatory_class="promoter"
     CDS             5291..6412
                     /codon_start=1
                     /gene="repA"
                     /product="RepA"
                     /label=repA
                     /note="required for replication"
                     /protein_id="AAO92601.1"
                     /translation="MISPESLNTNVSLTLLTRELQTEIILGTYTVRVHTRIGREPCARL
                     WYLCRALDKDGSGHLTLPLPVVQTFLDCSDKSVYRWLQDGKKIGAFRRYKIKAGMITVY
                     LGGMFQVCYNLNLKRWGDVAVVPLVQVLSDLRSLTTGIVTQSFQQKSRYAANRQLKPEY
                     RKLFGAPHPNELVKDTRQSSLKSPEGEVPCVLHISSSRIFVSKSFIHYGTSQKAVSCEL
                     GIHKRTVRRHQKQLGMNRRQLCQAKIEYNQLRHARNNDASEFWAFTGTKTDIGYQVMGD
                     AVVFSDGIAPGAKKRQPNTYQIDATEFDGRLFKVGDKVFMNRCNIYREQFTLTTMSAAR
                     RKYHFKLSQCHFSENRAGRVGNRFVIGCHSGEI"
     gene            5291..6412
                     /gene="repA"
                     /label=repA
     CDS             complement(7033..7584)
                     /codon_start=1
                     /gene="int/res"
                     /product="integrase/resolvase"
                     /label=int/res
                     /protein_id="AAO92602.1"
                     /translation="MKVNGNGRAKILTSDELRRLFSDGFTTPRDRVLFGICLFTGCRVS
                     EALALQTTDIKGETLTFRKSTTKGKLKTRVVDIQPGLAALMADYHPKPGTLFPGMRGVS
                     DRLTRYAADKILRDAAKRIGLEGISTHSFRRTALNQMSSAGIPLRHIQEISGHNDLGTL
                     QRYLEVTPEQRRKAVSVIGF"
     gene            complement(7033..7584)
                     /gene="int/res"
                     /label=int/res
ORIGIN
        1 atcgtccatt ccgacagcat cgccagtcac tatggcgtgc tgctagcgct atatgcgttg
       61 atgcaatttc tatgcgcagt gggcgcatca cgctaaaaca ggggaatatc acgctcaacg
      121 cggtgctggc cgaggagatt aacccgccca agggtgagac cccgttgaaa tggttgttgc
      181 tgaccggcga accggtcgag tcgctagccc aagccttgcg cgtcatcgac atttataccc
      241 atcgctggcg gatcgaggag ttccataagg catggaaaac cggagcagga gccgagaggc
      301 aacgcatgga ggagccggat aatctggagc ggatggtctc gatcctctcg tttgttgcgg
      361 tcaggctgtt acagctcaga gaaagcttca cgctgccgca agcactcagg gcgcaagggc
      421 tgctaaagga agcggaacac gtagaaagcc agtccgcaga aacggtgctg accccggatg
      481 aatgtcagct actgggctat ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta
      541 gcttgcagtg ggcttacatg gcgatagcta gactgggcgg ttttatggac agcaagcgaa
      601 ccggaattgc cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg
      661 atggctttct tgccgccaag gatctgatgg cgcaggggat caagatctga tcaagagaca
      721 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
      781 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
      841 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
      901 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
      961 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     1021 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     1081 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     1141 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     1201 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     1261 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     1321 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     1381 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     1441 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     1501 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     1561 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
     1621 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     1681 tcatgctgga gttcttcgcc caccggcatc accggcgcca caggtgcggt tgctggcgcc
     1741 tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
     1801 tgtttcggcg tgggtatggt ggcaggcccg ctttatcaga agccagacat taacgcttct
     1861 ggagaaactc aacgagctgg acgcggatga acaggcagac atctgtgaat cgcttcacga
     1921 ccacgctgat gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
     1981 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     2041 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
     2101 gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta
     2161 ctgagagtgc accatatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     2221 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     2281 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     2341 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     2401 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2461 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     2521 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     2581 tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt
     2641 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     2701 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     2761 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     2821 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     2881 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     2941 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     3001 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     3061 aagggatttt ggtcatgaga ttatcaaaag atcatcgatt tngtggtgat ggtgatgatg
     3121 gcaggaagag ccggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
     3181 caaatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat
     3241 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     3301 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     3361 tcccagtcac gacgttgtaa aacgacggcc agtgccaagc ttgcatgcct gcaggtcgac
     3421 tctagaggat ccccgggtac cgagctcgaa ttcgtaatca tggtcatatg ttctcctaac
     3481 ctgtagtttt atttttctta tttcatttta aataaaatcg acaccaaata tgacaatctg
     3541 tcatacttcg ggcaaatttt ttttgagatc gcgataaatc cagggaaaaa tgctctgcga
     3601 tcgctaaaaa tcactataat tacatggcac taaaaaactt atggctaaaa atacatcaat
     3661 atatgcagcc agagcgacaa attaaatttt ttatttatgt gatgtagata aaagatatct
     3721 ttcccctata cccctacact ctccttcaag tcatagaggc gcggagattg cccaatttta
     3781 ttagctgtgt actcagtacc tcagcaaaaa attctgagta ctgtgttctg agtcctgtgc
     3841 tcgagatgac ctgcaggtcg agaatagtta gcgcgcagtt cgacgggtga ttggtcaaac
     3901 gcagcggttc ccaagcgaga ggcacgacag taactggcat ttgcctaata ctccttgtta
     3961 aaccaatgtt tttagataga gcaagacccg gagaagtaag tagaacccaa cacttaagct
     4021 gaaaaccact tacccatccg ggcctagact aagcaaaatt agctgagggc gttaattgcg
     4081 tagtcgatgt agttgttagt ttcagtagca gcatcgccag tcaaacggga attcttgaag
     4141 acgaaagggc ctcttgcggg atatccgtct agatagtcat cgctcatccc tcacctcaac
     4201 cgttttgctg cctaacgagg tagtctaaga ccgcgccaat tctgtcgagt gccgcgctag
     4261 tggtacgttg gtactcatca agccgtgctt cggtttgtaa ttgcctagcg gtaagaattt
     4321 caaagttctg ctgatggatt tgaactgttt gcaacaatgc ttctgcggtt gcgcggagtt
     4381 ctgtaaccga tgtgcggagg tcggcattca ctgcaacttg gctgtctaac gcagcttcta
     4441 ttctggctag tctgtcttcg ctcatgctgc cccctgcttg tcttggttgt ccttggcttc
     4501 ttgttccatt tgttctttaa caactcgtgt gagtaagaaa gcggcaagat tagccaacgg
     4561 tcttccttcc tcagacgctc ggttagcaat ccattggtaa atttcagctt ctagagtgac
     4621 tgctattctc cctttattgg ttggcatgac gtttggtgta ctcctgagtc catatcatac
     4681 accttgtgca aagagtgatt gacaaatgta ctaatcggca gtaatagtat agagtgtatt
     4741 aagtgtacaa ggataatttt ctcaaagcgg aatggtgtta aacccgatta ttagagtcgt
     4801 catagcaaaa gcttgaaaac tggcaagggt gtactggttt tcgttctttt tggttctaat
     4861 ggttattttt taccagttta tttagtaata accgagtgcc aagtttcttg ttttacatag
     4921 caaagttagc aaaaaacatt ttgccgtgaa tttccatctg taaaaagcgt cacggcgaaa
     4981 ttcagtattt agtaaataca aaaataagtc aaacgttgat catgcaacac tttggcgtac
     5041 aattgattga aaattttgcc gataggcaaa gtttaaatta cctattaaaa aaagttcagt
     5101 gcttggaggt tgttatgcta cttaaccgaa gctaaataaa acacacatac acagaattgt
     5161 taccacacca aacaccaaac agaaaaaacg tgctttgaat ttatcaatta atgggtgtag
     5221 ttaacaagtt ttatttagct tcaggttaag caaataacat tcaataggta ctggactgag
     5281 acatttccaa atgatcagcc cagaaagctt aaatacaaat gtatcactaa ccctgcttac
     5341 aagggaacta caaactgaaa taattcttgg cacttacacg gtcagggtac acactcgcat
     5401 tggtcgagaa ccttgtgcgc ggttgtggta tttgtgtcga gcgcttgata aggatgggtc
     5461 tggtcattta acattgccat tgcctgtagt acagacgttc ttagattgta gtgataagtc
     5521 tgtttaccgt tggttacagg atggaaaaaa aataggagca ttccgccgct acaaaattaa
     5581 agcaggaatg ataaccgtct acttgggcgg tatgtttcaa gtttgttaca acctaaattt
     5641 aaaaagatgg ggtgatgttg cagtagtgcc tttagtccaa gtgttaagcg atttacgctc
     5701 actgaccact ggcatagtca cacaaagctt tcaacaaaag agccgctatg cggccaacag
     5761 acaacttaaa ccagagtaca gaaaactttt tggagcgcca cacccgaacg agctagtgaa
     5821 ggacacaagg caatcctctc ttaaatcgcc agagggggaa gtaccttgtg tacttcacat
     5881 tagttcgtcg cggatattcg tctcaaaaag tttcatccac tatggtacat ctcaaaaagc
     5941 tgtcagctgt gagttgggta ttcacaagcg cacagtgcga cgacatcaaa agcagctggg
     6001 catgaatcgc cgtcaacttt gccaagccaa aattgagtac aatcaactcc ggcacgctcg
     6061 gaataatgac gcgtctgaat tttgggcttt cactggaacc aaaactgata tcggttatca
     6121 agttatgggt gacgcggttg tgttttctga tggcattgcc cctggagcca aaaagagaca
     6181 gcccaacact taccaaattg acgcgactga atttgacggt cgattattca aagtgggtga
     6241 caaggtgttc atgaatcggt gcaacatcta ccgcgaacaa ttcaccctaa caacgatgtc
     6301 agcagcacgg cggaagtacc atttcaaact gtcacagtgc cacttcagcg aaaatcgagc
     6361 cggtcgggta ggcaaccgtt ttgtaatagg ttgccactct ggcgagatat gaaggacatt
     6421 tttaagaatt tttttttagc gtgaaatgct gcggcaacca gtatagtgat cgacacgaac
     6481 tagtgcagca tgaccgagag cctatagggt cgcggctttt tggcaataga gatcgtatgc
     6541 agatcatgcg tagtactttc ttcgacctta accccaaaga aagcttatta gatggagaag
     6601 gtcacaatta gaaaatattc atcgtcccga ttgaccctta acctttaatc ggtttccact
     6661 tgtaaaaaat tcggcgagcc attcttctta accctcagag tcgaacttca tacaagaata
     6721 tacaaaccct ttgggtttac cactaaaaca acggcgataa aaacctaata ggtttccaca
     6781 gaaacgtcta aggatttagg cgtgttgttt tggggcaatt tgagaaaata ttaaaccagt
     6841 cacaagttaa cttgtcgaag aaatattgca gcattctgca acacagggaa aggtataacg
     6901 tttccaaagt ccggaacgtg tgcagagttc tagaaccgat ggatacttta aaagtatcct
     6961 caagtgtaga gcgtgtgttt aggctgcgtc tttttttagc acccataggg gtctaaacag
     7021 cgttggcgta cattagaagc caatcacgga tacagctttg cgtcgctgtt cgggtgtaac
     7081 ttcaagatag cgttgcagtg tgccaaggtc attgtgaccg gatatctctt gaatgtgtcg
     7141 caacgggata ccggcgctag acatttggtt gagggcagta cggcggaaac tgtgggtact
     7201 gatgccttct agcccgattc ttttggctgc atcgcgcaag attttatccg ccgcgtatcg
     7261 cgtgagccta tcgctgactc ccctcatgcc agggaacagg gttcccggtt tggggtgata
     7321 gtcagccatg agtgcggcta gtcctggctg gatgtcaacc acgcgggttt tgagtttccc
     7381 tttggtggta gacttcctaa aggttagtgt ttcgccttta atgtccgttg tttggagtgc
     7441 tagagcttca ctaacgcggc aaccggtgaa tagacagatg ccaaacaaaa cgcgatcgcg
     7501 cggtgtggtg aatccgtcgc taaacagtcg cctgagttcg tcggaggtga gtattttggc
     7561 tcgtccgttg ccgttaacct tcataaccct tacagattaa gcattccaat actctacacg
     7621 ttatctgcat tttgtggaat tggtggctat atctattgat tcttcgtatg ctttctgttg
     7681 aaagccagtg ctagtttagc caagcttatc aatcgttgca cggtaaaatc aggctttcga
     7741 cgtaaggaca gaattttgat aacttcagct tatgttgtct cctgaagaaa gcaactcttt
     7801 gataaacgcg tcgatggctt tcccggcggc tgactgccta cccattttta gcttattgag
     7861 ggtgcgatcg cgggttgcct ccaatttgtc tagattgtct atactattgt ctatattctt
     7921 tctgtcttgc ccggtagctt gtccttctgc ctgaactacc gggctttcga ttttccccgc
     7981 agttcctcca gttgcgattg aagtttgtct agacgtgttg ctagattatc ttctatacgc
     8041 ttgtctataa attcatctat attgtctaga ttattagttg tgactttatc tagacgttgt
     8101 tcaatattgt ctatacgctg ttcgagggat gtgtctagat gggtgggagt gtgagaagtg
     8161 ggagtattaa acctgttacc tgccaaatac caacgaacaa attctgttaa cagtgctgag
     8221 gcgttggaac tttcagaact agcaaggttt ttaaaagact cccattcttc ggagtcaata
     8281 cgaaaagtgg ctagagtttt gtctgccatg attagataat gtctatactt gtatagatag
     8341 cagtataaac tattgtctat attagtgtct atataagtct agaa
//
