pALE4 vector (V009526)

LOCUS       V009526                 6872 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009526
VERSION     V009526
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6872)
  AUTHORS   Fortune SM, Jaeger A, Sarracino DA, Chase MR, Sassetti CM, Sherman
            DR, Bloom BR, Rubin EJ.
  TITLE     Mutually dependent secretion of proteins required for mycobacterial
            virulence
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 102 (30), 10676-10681 (2005)
   PUBMED   16030141
REFERENCE   2  (bases 1 to 6872)
  AUTHORS   Fortune SM, Jaeger A, Rubin EJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2005) Immunology and Infectious Diseases, Harvard
            School of Public Health, 200 Longwood Ave., Armenise 439, Boston, MA
            02139, USA
REFERENCE   3  (bases 1 to 6872)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6872)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2005"; volume: "102"; issue: "30"; pages:
            "10676-10681"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (19-MAY-2005) Immunology and Infectious Diseases, Harvard School of
            Public Health, 200 Longwood Ave., Armenise 439, Boston, MA 02139,
            USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1102..1188
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1280..1307
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     CDS             1847..3022
                     /gene="espA"
                     /label="ESX-1 secretion-associated protein EspA"
                     /note="ESX-1 secretion-associated protein EspA from
                     Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
                     Accession#: P9WJE1"
     CDS             3029..3046
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             3053..3082
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     terminator      3126..3173
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     rep_origin      complement(3377..3965)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4300..5112)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     CDS             complement(join(6299..6872,1..539))
                     /gene="33"
                     /label="Integrase"
                     /note="Integrase from Mycobacterium phage L5. Accession#:
                     P22884"