LOCUS       V009526                 6872 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009526
VERSION     V009526
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6872)
  AUTHORS   Fortune SM, Jaeger A, Sarracino DA, Chase MR, Sassetti CM, Sherman
            DR, Bloom BR, Rubin EJ.
  TITLE     Mutually dependent secretion of proteins required for mycobacterial
            virulence
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 102 (30), 10676-10681 (2005)
   PUBMED   16030141
REFERENCE   2  (bases 1 to 6872)
  AUTHORS   Fortune SM, Jaeger A, Rubin EJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2005) Immunology and Infectious Diseases, Harvard
            School of Public Health, 200 Longwood Ave., Armenise 439, Boston, MA
            02139, USA
REFERENCE   3  (bases 1 to 6872)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6872)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2005"; volume: "102"; issue: "30"; pages:
            "10676-10681"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (19-MAY-2005) Immunology and Infectious Diseases, Harvard School of
            Public Health, 200 Longwood Ave., Armenise 439, Boston, MA 02139,
            USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1102..1188
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1280..1307
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     CDS             1847..3022
                     /gene="espA"
                     /label="ESX-1 secretion-associated protein EspA"
                     /note="ESX-1 secretion-associated protein EspA from
                     Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
                     Accession#: P9WJE1"
     CDS             3029..3046
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             3053..3082
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     terminator      3126..3173
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     rep_origin      complement(3377..3965)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4300..5112)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     CDS             complement(join(6299..6872,1..539))
                     /gene="33"
                     /label="Integrase"
                     /note="Integrase from Mycobacterium phage L5. Accession#:
                     P22884"
ORIGIN
        1 tcctcaggcg tcagcgcctc tacgtcgcgc tcatcggctg ccttctgctc gatccggcac
       61 gggttctctg cgatcagctt gtcctcgacc gctgtgttca tcaccgcccg gaggacgttg
      121 taggcatgcc ggcgggcagt cgggtgcttc ctacccatcc cggcccacca cgcacgcacc
      181 agagctggcg tcatctctgt gaccgccact tcacctagca ccgggtagat gcggcgctcc
      241 gcgtgcccgc tgtacagatc cctggtgccg tctgcgaggt cgcgctccac gagccacttc
      301 cgggtgtact cctccagcgt gatggcgctg gcggctgcct tcttcgcccg gtcctgtgga
      361 ggggtccagg tctccatctc gatgagccgc ttctcgcccg cgagccaggc ttcggcgtcc
      421 atcttgttgt cgtaggtctg cagcgcgtag tacctcacac cgtcctgcgg gttgacgtat
      481 gaggcttgga tcctcccgct gcgctgagtc ttcagcgatc cccatccgcg acgtgccaac
      541 taggtctcct ctcgtcgtga acaaggctac cgggttgcaa ctcctgtgca actctcaggc
      601 ttcaacgcgc ttctacgacc tgcaatttct ttccacttag aggatgcagc cgagaggggg
      661 taaaaaccta tcttgaccgg cccatatatg tggtcggcag acacccattc ttccaaacta
      721 gctacgcggg ttcgattccc gtcgcccgct ccgctggtca gagggtgttt tcgccctctg
      781 gccatttttc tttccagggg tctgcaactc ttgtgcgact cttctgacct gggcatacgc
      841 ggttgcaacg catccctgat ctggctactt tcgatgctga caaacgaata gagccccccg
      901 cctgcgcgaa cagacgaggg gcattcacac cagattggag ctggtgcagt gaagagaata
      961 gaccgggaca aggttgcacc gggagttgca gcggtcggaa ccctcgccgt cggcgggctg
     1021 gcgttcgccc tgtcgttcac ggctctagcg ccgatggtag tgtggggtct ccccatgcga
     1081 gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     1141 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg
     1201 gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact
     1261 gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa
     1321 actcttcctg tcgtcatatc tagagtttag cggacgcgat ttaggacaac cgagatttgc
     1381 tttgcctcgc aaccatgaga gcgccccgct tcgacgccga atcgggtgag tgatggtggg
     1441 ttagcacagc cctgattgcg ccaccggcga ggtgattgtg cccgccacga ggccgccgcc
     1501 ggctagcccc atgagcacac tatatagact ctcctgcaac agatctcata ccgatcgaag
     1561 gcgaagcgca ggcatcgacg tcggagacac tgccttggga tcgcgccgcc tacacggcgg
     1621 ttggcgcatt gtcgcagcgc agttgcagga gggcaaatgt gcgcagacga tgtagtcgac
     1681 aacaagtgaa catgccgtct tcacgaactc aaaactgacg atctgcttag catgaaaaaa
     1741 actgttgaca tcggccaagc atgacagcca gactgtaggc cctacgcgtg caatgcagaa
     1801 ccaaggctat gcatggaatc gacgaccgtt gagataggcg gcaggcatga gcagagcgtt
     1861 catcatcgat ccaacgatca gtgccattga cggcttgtac gaccttctgg ggattggaat
     1921 acccaaccaa gggggtatcc tttactcctc actagagtac ttcgaaaaag ccctggagga
     1981 gctggcagca gcgtttccgg gtgatggctg gttaggttcg gccgcggaca aatacgccgg
     2041 caaaaaccgc aaccacgtga attttttcca ggaactggca gacctcgatc gtcagctcat
     2101 cagcctgatc cacgaccagg ccaacgcggt ccagacgacc cgcgacatcc tggagggcgc
     2161 caagaaaggt ctcgagttcg tgcgcccggt ggctgtggac ctgacctaca tcccggtcgt
     2221 cgggcacgcc ctatcggccg ccttccaggc gccgttttgc gcgggcgcga tggccgtagt
     2281 gggcggcgcg cttgcctact tggtcgtgaa aacgctgatc aacgcgactc aactcctcaa
     2341 attgcttgcc aaattggcgg agttggtcgc ggccgccatt gcggacatca tttcggatgt
     2401 ggcggacatc atcaagggca ccctcggaga agtgtgggag ttcatcacaa acgcgctcaa
     2461 cggcctgaaa gagctttggg acaagctcac ggggtgggtg accggactgt tctctcgagg
     2521 gtggtcgaac ctggagtcct tctttgcggg cgtccccggc ttgaccggcg cgaccagcgg
     2581 cttgtcgcaa gtgactggct tgttcggtgc ggccggtctg tccgcatcgt cgggcttggc
     2641 tcacgcggat agcctggcga gctcagccag cttgcccgcc ctggccggca ttgggggcgg
     2701 gtccggtttt gggggcttgc cgagcctggc tcaggtccat gccgcctcaa ctcggcaggc
     2761 gctacggccc cgagctgatg gcccggtcgg cgccgctgcc gagcaggtcg gcgggcagtc
     2821 gcagctggtc tccgcgcagg gttcccaagg tatgggcgga cccgtaggca tgggcggcat
     2881 gcacccctct tcgggggcgt cgaaagggac gacgacgaag aagtactcgg aaggcgcggc
     2941 ggcgggcact gaagacgccg agcgcgcgcc agtcgaagct gacgcgggcg gtgggcaaaa
     3001 ggtgctggta cgaaacgtcg tcactagtca ccaccaccac caccatatgg ccgagcagaa
     3061 gctgatctcg gaggaggacc tgtgacttaa gcttatcgat gtcgacgtag ttaactgagc
     3121 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
     3181 gaggaactat atccggagat ctggtaccgc ggccgcacgc gttgcgctcg gtcgttcggc
     3241 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     3301 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     3361 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     3421 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     3481 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     3541 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg
     3601 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     3661 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     3721 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     3781 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     3841 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     3901 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     3961 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac tagtgaggtc
     4021 tgcctcgtga agaaggtgtt gctgactcat accaggcctg aatcgcccca tcatccagcc
     4081 agaaagtgag ggagccacgg ttgatgagag ctttgttgta ggtggaccag ttggtgattt
     4141 tgaacttttg ctttgccacg gaacggtctg cgttgtcggg aagatgcgtg atctgatcct
     4201 tcaactcagc aaaagttcga tttattcaac aaagccgccg tcccgtcaag tcagcgtaat
     4261 gctctgccag tgttacaacc aattaaccaa ttctgattag aaaaactcat cgagcatcaa
     4321 atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt
     4381 ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg
     4441 gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat
     4501 aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag
     4561 attatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc
     4621 actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg
     4681 atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc
     4741 cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt
     4801 tttcccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt
     4861 gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac
     4921 atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat ggggcttccc
     4981 atacaagcga tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc
     5041 atataaatca gcatccatgt tggaatttaa tcgcggcctc gagcaagacg tttcccgttg
     5101 aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca
     5161 tgatgatata tttttatctt gtgcaatgta acatcagaga ttttgagaca caacgtcgct
     5221 ttgttggcta gctcacacaa ccggtcgtga cttttagggc tccgagagaa gctcctcgat
     5281 gtcgtctggc cacgaccaga ggagttcacc ctcggcggtg aggttggtgt gctcgttcac
     5341 ccggatcagg agatcgtcat cctcgatgcc tcgggggacg tacctgaacc cgccgccggc
     5401 cataccttcg tagggctcga tggatgggtc gaactcgagc actaagtcgt cgtcgcggag
     5461 catcttccac cacgacaata ggcgcttctt cttgtcttcg gacatcgtgc ggaagctacc
     5521 cactcgcatg tactcgccgt gatcccggag cctctgaaaa gccttcgact tatcgtgagg
     5581 tttccgcgtg tcccacggcc agttctgctg gacgatctgc ctggtggtca accgtcctcc
     5641 gtaggtcttc ttgtgccacg acaccgcttg tcgagtcacg ccatacagct ctgcgatttc
     5701 ggtctgatta aaccccttcc tgcgaagatc ttcgatctcg ctgagagtga gtggtattcg
     5761 gctaggggcc ggaaccactg ctttgtgttg gattttgccg ctcatgtttc cctccatgag
     5821 aaaggtgcgt gcgtctccgc cgattacgga gacatgttgg tgcctgtcaa ggatacccct
     5881 aatttagttg cgtctgcgga accatattca gttgtgttcc ccgacgccgt ggccgtctcc
     5941 cactgggcgt gggatcgact ggcgttacgc ggtcgtaaat gtagcggcct gccccactcg
     6001 gtagcaaacc ttgtgacagg tatcacttag gtcgccttct gttacacgtt gacctcgggt
     6061 ttcatcgtca cgactctcct ttcttagaca gcctcaagat cgttacaccg gcttgcgaag
     6121 atgtaccttc tagctggcgg gaacggcaat gctcctggcg gcggcggggc tggtgcccag
     6181 gtctccgcac agagcggcgg tgctggcgct cgcggccagg cgtggttctt cgcgtactga
     6241 caagaaaccc ccctctttag gactcagtgt ccttgggagg ggggcttttt gcgtttcagg
     6301 aggtcttggc cagcttggac atcgcctcag cgatagcctc gtcgcgggcc tcagacgcca
     6361 tctggtactt catcgccatc ctaggagtcg tgtgaccgag acgggccatc agctccttgg
     6421 tcgtcgcacc tgcctgagcg gcgaacgtag cgccgacagc gcggaggtcg tggatgcgga
     6481 gttccggccg accgatcttg gcgtagccac gcttcagcga cttggtgaac gcggacttcg
     6541 acagccggtt gccctgcgtc gtggtcacca ggaatgcctc ggggcccttg ttcatcttcg
     6601 tacggtcctt catgtgcgct cggatcatct ccgcgacgtg aggcggaacc gtcacaggac
     6661 gcttcgaccg gacggtcttg gcgttgccaa cgacgatctt gttccccacg cgggaagcgc
     6721 cacggcgcac ccggagcttc atcgtcatgc cgtcgtgcac gatgtccttg cggcgaagct
     6781 cgatcagctc tccgaaccgg aggctcgtcc acgccaggat gtatgccgcg atccggtagt
     6841 gctcgaagat ctcagcggcg acgatgtcca gc
//
