pALE3 vector (V009527)

LOCUS       V009527                 6815 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009527
VERSION     V009527
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6815)
  AUTHORS   Fortune SM, Jaeger A, Sarracino DA, Chase MR, Sassetti CM, Sherman
            DR, Bloom BR, Rubin EJ.
  TITLE     Mutually-dependent secretion of proteins required for mycobacterial
            virulence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6815)
  AUTHORS   Fortune SM, Jaeger A.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2005) Immunology and Infectious Diseases, Harvard
            School of Public Health, 200 Longwood Ave., Armenise 439, Boston, MA
            02139, USA
REFERENCE   3  (bases 1 to 6815)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6815)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (19-MAY-2005) Immunology and Infectious Diseases, Harvard School of
            Public Health, 200 Longwood Ave., Armenise 439, Boston, MA 02139,
            USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      52..138
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      230..257
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     5'UTR           295..796
     CDS             797..1972
                     /gene="espA"
                     /label="ESX-1 secretion-associated protein EspA"
                     /note="ESX-1 secretion-associated protein EspA from
                     Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
                     Accession#: P9WJE1"
     terminator      2019..2066
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     rep_origin      complement(2270..2858)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3193..4005)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     CDS             complement(5192..6304)
                     /gene="33"
                     /label="Integrase"
                     /note="Integrase from Mycobacterium phage L5. Accession#:
                     P22884"