LOCUS       V009527                 6815 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009527
VERSION     V009527
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6815)
  AUTHORS   Fortune SM, Jaeger A, Sarracino DA, Chase MR, Sassetti CM, Sherman
            DR, Bloom BR, Rubin EJ.
  TITLE     Mutually-dependent secretion of proteins required for mycobacterial
            virulence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6815)
  AUTHORS   Fortune SM, Jaeger A.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2005) Immunology and Infectious Diseases, Harvard
            School of Public Health, 200 Longwood Ave., Armenise 439, Boston, MA
            02139, USA
REFERENCE   3  (bases 1 to 6815)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6815)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (19-MAY-2005) Immunology and Infectious Diseases, Harvard School of
            Public Health, 200 Longwood Ave., Armenise 439, Boston, MA 02139,
            USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      52..138
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      230..257
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     5'UTR           295..796
     CDS             797..1972
                     /gene="espA"
                     /label="ESX-1 secretion-associated protein EspA"
                     /note="ESX-1 secretion-associated protein EspA from
                     Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
                     Accession#: P9WJE1"
     terminator      2019..2066
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     rep_origin      complement(2270..2858)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3193..4005)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     CDS             complement(5192..6304)
                     /gene="33"
                     /label="Integrase"
                     /note="Integrase from Mycobacterium phage L5. Accession#:
                     P22884"
ORIGIN
        1 ccgatggtag tgtggggtct ccccatgcga gagtagggaa ctgccaggca tcaaataaaa
       61 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
      121 ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga
      181 gggtggcggg caggacgccc gccataaact gccaggcatc aaattaagca gaaggccatc
      241 ctgacggatg gcctttttgc gtttctacaa actcttcctg tcgtcatatc tagagtttag
      301 cggacgcgat ttaggacaac cgagatttgc tttgcctcgc aaccatgaga gcgccccgct
      361 tcgacgccga atcgggtgag tgatggtggg ttagcacagc cctgattgcg ccaccggcga
      421 ggtgattgtg cccgccacga ggccgccgcc ggctagcccc atgagcacac tatatagact
      481 ctcctgcaac agatctcata ccgatcgaag gcgaagcgca ggcatcgacg tcggagacac
      541 tgccttggga tcgcgccgcc tacacggcgg ttggcgcatt gtcgcagcgc agttgcagga
      601 gggcaaatgt gcgcagacga tgtagtcgac aacaagtgaa catgccgtct tcacgaactc
      661 aaaactgacg atctgcttag catgaaaaaa actgttgaca tcggccaagc atgacagcca
      721 gactgtaggc cctacgcgtg caatgcagaa ccaaggctat gcatggaatc gacgaccgtt
      781 gagataggcg gcaggcatga gcagagcgtt catcatcgat ccaacgatca gtgccattga
      841 cggcttgtac gaccttctgg ggattggaat acccaaccaa gggggtatcc tttactcctc
      901 actagagtac ttcgaaaaag ccctggagga gctggcagca gcgtttccgg gtgatggctg
      961 gttaggttcg gccgcggaca aatacgccgg caaaaaccgc aaccacgtga attttttcca
     1021 ggaactggca gacctcgatc gtcagctcat cagcctgatc cacgaccagg ccaacgcggt
     1081 ccagacgacc cgcgacatcc tggagggcgc caagaaaggt ctcgagttcg tgcgcccggt
     1141 ggctgtggac ctgacctaca tcccggtcgt cgggcacgcc ctatcggccg ccttccaggc
     1201 gccgttttgc gcgggcgcga tggccgtagt gggcggcgcg cttgcctact tggtcgtgaa
     1261 aacgctgatc aacgcgactc aactcctcaa attgcttgcc aaattggcgg agttggtcgc
     1321 ggccgccatt gcggacatca tttcggatgt ggcggacatc atcaagggca ccctcggaga
     1381 agtgtgggag ttcatcacaa acgcgctcaa cggcctgaaa gagctttggg acaagctcac
     1441 ggggtgggtg accggactgt tctctcgagg gtggtcgaac ctggagtcct tctttgcggg
     1501 cgtccccggc ttgaccggcg cgaccagcgg cttgtcgcaa gtgactggct tgttcggtgc
     1561 ggccggtctg tccgcatcgt cgggcttggc tcacgcggat agcctggcga gctcagccag
     1621 cttgcccgcc ctggccggca ttgggggcgg gtccggtttt gggggcttgc cgagcctggc
     1681 tcaggtccat gccgcctcaa ctcggcaggc gctacggccc cgagctgatg gcccggtcgg
     1741 cgccgctgcc gagcaggtcg gcgggcagtc gcagctggtc tccgcgcagg gttcccaagg
     1801 tatgggcgga cccgtaggca tgggcggcat gcacccctct tcgggggcgt cgaaagggac
     1861 gacgacgaag aagtactcgg aaggcgcggc ggcgggcact gaagacgccg agcgcgcgcc
     1921 agtcgaagct gacgcgggcg gtgggcaaaa ggtgctggta cgaaacgtcg tctgagatct
     1981 taagcttatc gatgtcgacg tagttaactg agcaataact agcataaccc cttggggcct
     2041 ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga gatctggtac
     2101 cgcggccgca cgcgttgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     2161 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     2221 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     2281 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     2341 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     2401 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     2461 ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     2521 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     2581 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     2641 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     2701 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     2761 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     2821 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     2881 cggggtctga cgctcagtgg aactagtgag gtctgcctcg tgaagaaggt gttgctgact
     2941 cataccaggc ctgaatcgcc ccatcatcca gccagaaagt gagggagcca cggttgatga
     3001 gagctttgtt gtaggtggac cagttggtga ttttgaactt ttgctttgcc acggaacggt
     3061 ctgcgttgtc gggaagatgc gtgatctgat ccttcaactc agcaaaagtt cgatttattc
     3121 aacaaagccg ccgtcccgtc aagtcagcgt aatgctctgc cagtgttaca accaattaac
     3181 caattctgat tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg
     3241 attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag
     3301 gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc
     3361 aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg
     3421 agtgacgact gaatccggtg agaatggcaa aagattatgc atttctttcc agacttgttc
     3481 aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat
     3541 tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac
     3601 aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga
     3661 atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa
     3721 ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt
     3781 cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg
     3841 tttcagaaac aactctggcg catggggctt cccatacaag cgatagattg tcgcacctga
     3901 ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt
     3961 taatcgcggc ctcgagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt
     4021 actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat
     4081 gtaacatcag agattttgag acacaacgtc gctttgttgg ctagctcaca caaccggtcg
     4141 tgacttttag ggctccgaga gaagctcctc gatgtcgtct ggccacgacc agaggagttc
     4201 accctcggcg gtgaggttgg tgtgctcgtt cacccggatc aggagatcgt catcctcgat
     4261 gcctcggggg acgtacctga acccgccgcc ggccatacct tcgtagggct cgatggatgg
     4321 gtcgaactcg agcactaagt cgtcgtcgcg gagcatcttc caccacgaca ataggcgctt
     4381 cttcttgtct tcggacatcg tgcggaagct acccactcgc atgtactcgc cgtgatcccg
     4441 gagcctctga aaagccttcg acttatcgtg aggtttccgc gtgtcccacg gccagttctg
     4501 ctggacgatc tgcctggtgg tcaaccgtcc tccgtaggtc ttcttgtgcc acgacaccgc
     4561 ttgtcgagtc acgccataca gctctgcgat ttcggtctga ttaaacccct tcctgcgaag
     4621 atcttcgatc tcgctgagag tgagtggtat tcggctaggg gccggaacca ctgctttgtg
     4681 ttggattttg ccgctcatgt ttccctccat gagaaaggtg cgtgcgtctc cgccgattac
     4741 ggagacatgt tggtgcctgt caaggatacc cctaatttag ttgcgtctgc ggaaccatat
     4801 tcagttgtgt tccccgacgc cgtggccgtc tcccactggg cgtgggatcg actggcgtta
     4861 cgcggtcgta aatgtagcgg cctgccccac tcggtagcaa accttgtgac aggtatcact
     4921 taggtcgcct tctgttacac gttgacctcg ggtttcatcg tcacgactct cctttcttag
     4981 acagcctcaa gatcgttaca ccggcttgcg aagatgtacc ttctagctgg cgggaacggc
     5041 aatgctcctg gcggcggcgg ggctggtgcc caggtctccg cacagagcgg cggtgctggc
     5101 gctcgcggcc aggcgtggtt cttcgcgtac tgacaagaaa cccccctctt taggactcag
     5161 tgtccttggg aggggggctt tttgcgtttc aggaggtctt ggccagcttg gacatcgcct
     5221 cagcgatagc ctcgtcgcgg gcctcagacg ccatctggta cttcatcgcc atcctaggag
     5281 tcgtgtgacc gagacgggcc atcagctcct tggtcgtcgc acctgcctga gcggcgaacg
     5341 tagcgccgac agcgcggagg tcgtggatgc ggagttccgg ccgaccgatc ttggcgtagc
     5401 cacgcttcag cgacttggtg aacgcggact tcgacagccg gttgccctgc gtcgtggtca
     5461 ccaggaatgc ctcggggccc ttgttcatct tcgtacggtc cttcatgtgc gctcggatca
     5521 tctccgcgac gtgaggcgga accgtcacag gacgcttcga ccggacggtc ttggcgttgc
     5581 caacgacgat cttgttcccc acgcgggaag cgccacggcg cacccggagc ttcatcgtca
     5641 tgccgtcgtg cacgatgtcc ttgcggcgaa gctcgatcag ctctccgaac cggaggctcg
     5701 tccacgccag gatgtatgcc gcgatccggt agtgctcgaa gatctcagcg gcgacgatgt
     5761 ccagctcctc aggcgtcagc gcctctacgt cgcgctcatc ggctgccttc tgctcgatcc
     5821 ggcacgggtt ctctgcgatc agcttgtcct cgaccgctgt gttcatcacc gcccggagga
     5881 cgttgtaggc atgccggcgg gcagtcgggt gcttcctacc catcccggcc caccacgcac
     5941 gcaccagagc tggcgtcatc tctgtgaccg ccacttcacc tagcaccggg tagatgcggc
     6001 gctccgcgtg cccgctgtac agatccctgg tgccgtctgc gaggtcgcgc tccacgagcc
     6061 acttccgggt gtactcctcc agcgtgatgg cgctggcggc tgccttcttc gcccggtcct
     6121 gtggaggggt ccaggtctcc atctcgatga gccgcttctc gcccgcgagc caggcttcgg
     6181 cgtccatctt gttgtcgtag gtctgcagcg cgtagtacct cacaccgtcc tgcgggttga
     6241 cgtatgaggc ttggatcctc ccgctgcgct gagtcttcag cgatccccat ccgcgacgtg
     6301 ccaactaggt ctcctctcgt cgtgaacaag gctaccgggt tgcaactcct gtgcaactct
     6361 caggcttcaa cgcgcttcta cgacctgcaa tttctttcca cttagaggat gcagccgaga
     6421 gggggtaaaa acctatcttg accggcccat atatgtggtc ggcagacacc cattcttcca
     6481 aactagctac gcgggttcga ttcccgtcgc ccgctccgct ggtcagaggg tgttttcgcc
     6541 ctctggccat ttttctttcc aggggtctgc aactcttgtg cgactcttct gacctgggca
     6601 tacgcggttg caacgcatcc ctgatctggc tactttcgat gctgacaaac gaatagagcc
     6661 ccccgcctgc gcgaacagac gaggggcatt cacaccagat tggagctggt gcagtgaaga
     6721 gaatagaccg ggacaaggtt gcaccgggag ttgcagcggt cggaaccctc gccgtcggcg
     6781 ggctggcgtt cgccctgtcg ttcacggctc tagcg
//
