pCB301 vector (V008699)

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LOCUS       40924_9116        3574 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pCB301, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Xiang C, Han P, Lutziger I, Wang K, Oliver DJ.
  TITLE     A mini binary vector series for plant transformation
  JOURNAL   Plant Mol. Biol. 40 (4), 711-717 (1999)
  PUBMED    10480394
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Xiang C, Han P, Lutziger I, Wang K, Oliver DJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-1999) Botany, Iowa State University, Bessey Hall, 
            Ames, IA 50011, USA
REFERENCE   3  (bases 1 to 3574)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3574)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol. 
            Biol."; date: "1999"; volume: "40"; issue: "4"; pages: "711-717"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-MAR-1999) Botany, Iowa State University, Bessey Hall, Ames, IA 
            50011, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3574
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..622
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     CDS             842..1633
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             1935..3080
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    complement(3235..3259)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    3300..3407
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     misc_feature    complement(3471..3495)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      3573..3574
                     /label=oriV
                     /note="incP origin of replication"