LOCUS       40924_9116        3574 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pCB301, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Xiang C, Han P, Lutziger I, Wang K, Oliver DJ.
  TITLE     A mini binary vector series for plant transformation
  JOURNAL   Plant Mol. Biol. 40 (4), 711-717 (1999)
  PUBMED    10480394
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Xiang C, Han P, Lutziger I, Wang K, Oliver DJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-1999) Botany, Iowa State University, Bessey Hall, 
            Ames, IA 50011, USA
REFERENCE   3  (bases 1 to 3574)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3574)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol. 
            Biol."; date: "1999"; volume: "40"; issue: "4"; pages: "711-717"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-MAR-1999) Botany, Iowa State University, Bessey Hall, Ames, IA 
            50011, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3574
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..622
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     CDS             842..1633
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             1935..3080
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    complement(3235..3259)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    3300..3407
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     misc_feature    complement(3471..3495)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      3573..3574
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 cgctcaccgg gctggttgcc ctcgccgctg ggctggcggc cgtctatggc cctgcaaacg
       61 cgccagaaac gccgtcgaag ccgtgtgcga gacaccgcgg ccgccggcgt tgtggatacc
      121 tcgcggaaaa cttggccctc actgacagat gaggggcgga cgttgacact tgaggggccg
      181 actcacccgg cgcggcgttg acagatgagg ggcaggctcg atttcggccg gcgacgtgga
      241 gctggccagc ctcgcaaatc ggcgaaaacg cctgatttta cgcgagtttc ccacagatga
      301 tgtggacaag cctggggata agtgccctgc ggtattgaca cttgaggggc gcgactactg
      361 acagatgagg ggcgcgatcc ttgacacttg aggggcagag tgctgacaga tgaggggcgc
      421 acctattgac atttgagggg ctgtccacag gcagaaaatc cagcatttgc aagggtttcc
      481 gcccgttttt cggccaccgc taacctgtct tttaacctgc ttttaaacca atatttataa
      541 accttgtttt taaccagggc tgcgccctgt gcgcgtgacc gcgcacgccg aaggggggtg
      601 cccccccttc tcgaaccctc ccggcccgct ctcgagttgg cagcatcacc cataattgtg
      661 gtttcaaaat cggctccgtc gatactatgt tatacgccaa ctttgaaaac aactttgaaa
      721 aagctgtttt ctggtattta aggttttaga atgcaaggaa cagtgaattg gagttcgtct
      781 tgttataatt agcttcttgg ggtatcttta aatactgtag aaaagaggaa ggaaataata
      841 aatggctaaa atgagaatat caccggaatt gaaaaaactg atcgaaaaat accgctgcgt
      901 aaaagatacg gaaggaatgt ctcctgctaa ggtatataag ctggtgggag aaaatgaaaa
      961 cctatattta aaaatgacgg acagccggta taaagggacc acctatgatg tggaacggga
     1021 aaaggacatg atgctatggc tggaaggaaa gctgcctgtt ccaaaggtcc tgcactttga
     1081 acggcatgat ggctggagca atctgctcat gagtgaggcc gatggcgtcc tttgctcgga
     1141 agagtatgaa gatgaacaaa gccctgaaaa gattatcgag ctgtatgcgg agtgcatcag
     1201 gctctttcac tccatcgaca tatcggattg tccctatacg aatagcttag acagccgctt
     1261 agccgaattg gattacttac tgaataacga tctggccgat gtggattgcg aaaactggga
     1321 agaagacact ccatttaaag atccgcgcga gctgtatgat tttttaaaga cggaaaagcc
     1381 cgaagaggaa cttgtctttt cccacggcga cctgggagac agcaacatct ttgtgaaaga
     1441 tggcaaagta agtggcttta ttgatcttgg gagaagcggc agggcggaca agtggtatga
     1501 cattgccttc tgcgtccggt cgatcaggga ggatatcggg gaagaacagt atgtcgagct
     1561 attttttgac ttactgggga tcaagcctga ttgggagaaa ataaaatatt atattttact
     1621 ggatgaattg ttttagtacc tagatgtggc gcaacgatgc cggcgacaag caggagcgca
     1681 ccgacttctt ccgcatcaag tgttttggct ctcaggccga ggcccacggc aagtatttgg
     1741 gcaaggggtc gctggtattc gtgcagggca agattcggaa taccaagtac gagaaggacg
     1801 gccagacggt ctacgggacc gacttcattg ccgataaggt ggattatctg gacaccaagg
     1861 caccaggcgg gtcaaatcag gaataagggc acattgcccc ggcgtgagtc ggggcaatcc
     1921 cgcaaggagg gtgaatgaat cggacgtttg accggaaggc atacaggcaa gaactgatcg
     1981 acgcggggtt ttccgccgag gatgccgaaa ccatcgcaag ccgcaccgtc atgcgtgcgc
     2041 cccgcgaaac cttccagtcc gtcggctcga tggtccagca agctacggcc aagatcgagc
     2101 gcgacagcgt gcaactggct ccccctgccc tgcccgcgcc atcggccgcc gtggagcgtt
     2161 cgcgtcgtct cgaacaggag gcggcaggtt tggcgaagtc gatgaccatc gacacgcgag
     2221 gaactatgac gaccaagaag cgaaaaaccg ccggcgagga cctggcaaaa caggtcagcg
     2281 aggccaagca ggccgcgttg ctgaaacaca cgaagcagca gatcaaggaa atgcagcttt
     2341 ccttgttcga tattgcgccg tggccggaca cgatgcgagc gatgccaaac gacacggccc
     2401 gctctgccct gttcaccacg cgcaacaaga aaatcccgcg cgaggcgctg caaaacaagg
     2461 tcattttcca cgtcaacaag gacgtgaaga tcacctacac cggcgtcgag ctgcgggccg
     2521 acgatgacga actggtgtgg cagcaggtgt tggagtacgc gaagcgcacc cctatcggcg
     2581 agccgatcac cttcacgttc tacgagcttt gccaggacct gggctggtcg atcaatggcc
     2641 ggtattacac gaaggccgag gaatgcctgt cgcgcctaca ggcgacggcg atgggcttca
     2701 cgtccgaccg cgttgggcac ctggaatcgg tgtcgctgct gcaccgcttc cgcgtcctgg
     2761 accgtggcaa gaaaacgtcc cgttgccagg tcctgatcga cgaggaaatc gtcgtgctgt
     2821 ttgctggcga ccactacacg aaattcatat gggagaagta ccgcaagctg tcgccgacgg
     2881 cccgacggat gttcgactat ttcagctcgc accgggagcc gtacccgctc aagctggaaa
     2941 ccttccgcct catgtgcgga tcggattcca cccgcgtgaa gaagtggcgc gagcaggtcg
     3001 gcgaagcctg cgaagagttg cgaggcagcg gcctggtgga acacgcctgg gtcaatgatg
     3061 acctggtgca ttgcaaacgc tagggccttg tggggtcagt tccggctggg ggttcagcca
     3121 gcgctttact gagatctcct gtggttggca tgcacataca aatggacgaa cggataaacc
     3181 ttttcacgcc cttttaaata tccgattatt ctaataaacg ctcttttctc ttaggtttac
     3241 ccgccaatat atcctgtcaa acactgatag tttaaactga aggcgggaaa cgacaatctg
     3301 agctccaccg cggtggcggc cgctctagaa ctagtggatc ccccgggctg caggaattcg
     3361 atatcaagct tatcgatacc gtcgacctcg agggggggcc cggtaccaaa accaccccag
     3421 tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca
     3481 caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca
     3541 ccactcgata caggcagccc atcagtccac taga
//
