pBlueLysis vector (V009146)

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LOCUS       V009146                 5443 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009146
VERSION     V009146
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5443)
  AUTHORS   Yun J, Kang S, Ryu S.
  TITLE     Development of a novel vector system for programmed cell lysis in
            Escherichia coli in response to glucose exhaustion
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5443)
  AUTHORS   Yun J, Kang S, Ryu S.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2004) School of Agricultural Biotechnology, Seoul
            National Univ., 200-8105 CALS Seoul National Univ. Kwanak-gu, Seoul
            151-742, South Korea
REFERENCE   3  (bases 1 to 5443)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5443)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (28-OCT-2004) School of Agricultural Biotechnology, Seoul National
            Univ., 200-8105 CALS Seoul National Univ. Kwanak-gu, Seoul 151-742,
            South Korea"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5443
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    143..164
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        179..209
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    217..233
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     241..257
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    complement(270..326)
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(327..343)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      complement(1008..1553)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     promoter        1665..1693
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             1741..2928
                     /label="TcR"
                     /note="tetracycline efflux protein"
     regulatory      3991..4170
                     /regulatory_class="promoter"
     CDS             4174..4821
                     /gene="t"
                     /label="Holin"
                     /note="Holin from Enterobacteria phage T4. Accession#:
                     P06808"
     CDS             4834..4851
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             4958..5410
                     /label="T7 lysozyme"
                     /note="lysozyme from bacteriophage T7"
     promoter        5411..5443
                     /label="T7 Phi-3.8 promoter"
                     /note="weak promoter for bacteriophage T7 RNA polymerase"