LOCUS       V009146                 5443 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009146
VERSION     V009146
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5443)
  AUTHORS   Yun J, Kang S, Ryu S.
  TITLE     Development of a novel vector system for programmed cell lysis in
            Escherichia coli in response to glucose exhaustion
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5443)
  AUTHORS   Yun J, Kang S, Ryu S.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2004) School of Agricultural Biotechnology, Seoul
            National Univ., 200-8105 CALS Seoul National Univ. Kwanak-gu, Seoul
            151-742, South Korea
REFERENCE   3  (bases 1 to 5443)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5443)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (28-OCT-2004) School of Agricultural Biotechnology, Seoul National
            Univ., 200-8105 CALS Seoul National Univ. Kwanak-gu, Seoul 151-742,
            South Korea"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5443
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    143..164
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        179..209
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    217..233
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     241..257
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    complement(270..326)
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(327..343)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      complement(1008..1553)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     promoter        1665..1693
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             1741..2928
                     /label="TcR"
                     /note="tetracycline efflux protein"
     regulatory      3991..4170
                     /regulatory_class="promoter"
     CDS             4174..4821
                     /gene="t"
                     /label="Holin"
                     /note="Holin from Enterobacteria phage T4. Accession#:
                     P06808"
     CDS             4834..4851
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             4958..5410
                     /label="T7 lysozyme"
                     /note="lysozyme from bacteriophage T7"
     promoter        5411..5443
                     /label="T7 Phi-3.8 promoter"
                     /note="weak promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
       61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagatccc gactggaaag
      121 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
      181 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
      241 caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc gactctagaa
      301 gatctccggg taccgagctc gaattcactg gccgtcgttt tacaacgtcg tgactgggaa
      361 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
      421 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
      481 tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
      541 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
      601 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
      661 gtgaccgtct ccgggatctg tccctcctgt tcagctactg acggggtggt gcgtaacggc
      721 aaaagcaccg ccggacatca gcgctagcgg agtgtatact ggcttactat gttggcactg
      781 atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa aaggctgcac cggtgcgtca
      841 gcagaatatg tgatacagga tatattccgc ttcctcgctc actgactcgc tacgctcggt
      901 cgttcgactg cggcgagcgg aaatggctta cgaacggggc ggagatttcc tggaagatgc
      961 caggaagata cttaacaggg aagtgagagg gccgcggcaa agccgttttt ccataggctc
     1021 cgcccccctg acaagcatca cgaaatctga cgctcaaatc agtggtggcg aaacccgaca
     1081 ggactataaa gataccaggc gtttccccct ggcggctccc tcgtgcgctc tcctgttcct
     1141 gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttgtctca ttccacgcct
     1201 gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg aaccccccgt
     1261 tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggaaagaca
     1321 tgcaaaagca ccactggcag cagccactgg taattgattt agaggagtta gtcttgaagt
     1381 catgcgccgg ttaaggctaa actgaaagga caagttttgg tgactgcgct cctccaagcc
     1441 agttacctcg gttcaaagag ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg
     1501 cggttttttc gttttcagag caagagatta cgcgcagacc aaaacgatct caagaagatc
     1561 atcttattaa tcagataaaa tatttctaga tttcagtgca atttatctct tcaaatgtag
     1621 cacctgaagt cagccccata cgatataagt tgtaattctc atgtttgaca gcttatcatc
     1681 gataagcttt aatgcggtag tttatcacag ttaaattgct aacgcagtca ggcaccgtgt
     1741 atgaaatcta acaatgcgct catcgtcatc ctcggcaccg tcaccctgga tgctgtaggc
     1801 ataggcttgg ttatgccggt actgccgggc ctcttgcggg atatcgtcca ttccgacagc
     1861 atcgccagtc actatggcgt gctgctagcg ctatatgcgt tgatgcaatt tctatgcgca
     1921 cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct cgcttcgcta
     1981 cttggagcca ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg gatcctctac
     2041 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc
     2101 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc
     2161 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat
     2221 gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta
     2281 atgcaggagt cgcataaggg agagcgtcga ccgatgccct tgagagcctt caacccagtc
     2341 agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt
     2401 atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc
     2461 tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc
     2521 ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt
     2581 atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc
     2641 tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg
     2701 caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc
     2761 gcggctctta ccagcctaac ttcgatcact ggaccgctga tcgtcacggc gatttatgcc
     2821 gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc
     2881 tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg aatggaagcc
     2941 ggcggcacct cgctaacgga ttcaccactc caagaattgg agccaatcaa ttcttgcgga
     3001 gaactgtgaa tgcgcaaacc aacccttggc agaacatatc catcgcgtcc gccatctcca
     3061 gcagccgcac gcggcgcatc tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg
     3121 tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg
     3181 aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag
     3241 caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtccc
     3301 ctacgtgctg ctgaagttgc ccgcaacaga gagtggaacc aaccggtgat accacgatac
     3361 tatgactgag agtcaacgcc atgagcggcc tcatttctta ttctgagtta caacagtccg
     3421 caccgctgtc cggtagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta
     3481 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgccc aacagtcccc
     3541 cggccacggg gcctgccacc atacccacgc cgaaacaagc gccctgcacc attatgttcc
     3601 ggatctgcat cgcaggatgc tgctggctac cctgtggaac acctacatct gtattaacga
     3661 agcgctaacc gtttttatca ggctctggga ggcagaataa atgatcatat cgtcaattat
     3721 tacctccacg gggagagcct gagcaaactg gcctcaggca tttgagaagc acacggtcac
     3781 actgcttccg gtagtcaata aaccggtaaa ccagcaatag acataagcgg ctatttaacg
     3841 accctgccct gaaccgacga ccgggtcgaa tttgctttcg aatttctgcc attcatccgc
     3901 ttattatcac ttattcaggc gtagcaccag gcgtttaagg gcaccaataa ctgccttaaa
     3961 aaaattacgc cccgccctgc cactcatcgc agtacttctc caatgatctg aagttgaaac
     4021 atgatagccg ttaaacaaat tggcactgaa ttattttact ctgtgtaata aataaagggc
     4081 gcttagatgc cctgtacacg gcgaggctct ccccccttgc cacgcgtgag aacgtaaaaa
     4141 aagcacccat actcaggagc actctcaacc atggcaccta gaatatcatt ttcgccctct
     4201 gatattctat ttggtgttct agatcgcttg ttcaaagata acgctaccgg gaaggttctt
     4261 gcttcccggg tagctgtcgt aattcttttg tttataatgg cgattgtttg gtatagggga
     4321 gatagtttct ttgagtacta taagcaatca aagtatgaaa catacagtga aattattgaa
     4381 aaggaaagaa ctgcacgctt tgaatctgtc gccctggaac aactccagat agttcatata
     4441 tcatctgagg cagactttag tgcggtgtat tctttccgcc ctaaaaactt aaactatttt
     4501 gttgatatta tagcatacga aggaaaatta ccttcaacaa taagtgaaaa atcacttgga
     4561 ggatatcctg ttgataaaac tatggatgaa tatacagttc atttaaatgg acgtcattat
     4621 tattccaact caaaatttgc ttttttacca actaaaaagc ctactcccga aataaactac
     4681 atgtacagtt gtccatattt taatttggat aatatctatg ctggaacgat aaccatgtac
     4741 tggtatagaa atgatcatat aagtaatgac cgccttgaat caatatgtgc tcaggcggcc
     4801 agaatattag gaagggctaa ataattactc gagcaccacc accaccacca ctgagatccg
     4861 gctgctaaca aagcccgaaa ggaagctgag tggctgctgc caccgctgag atccgggtcc
     4921 cctttgatag attaaaaagg aaaggaggaa agaaataatg gctcgtgtac agtttaaaca
     4981 acgtgaatct actgacgcaa tctttgttca ctgctcggct accaagccaa gtcagaatgt
     5041 tggtgtccgt gagattcgcc agtggcacaa agagcagggt tggctcgatg tgggatacca
     5101 ctttatcatc aagcgagacg gtactgtgga ggcaggacga gatgagatgg ctgtaggctc
     5161 tcacgctaag ggttacaacc acaactctat cggcgtctgc cttgttggtg gtatcgacga
     5221 taaaggtaag ttcgacgcta actttacgcc agcccaaatg caatcccttc gctcactgct
     5281 tgtcacactg ctggctaagt acgaaggcgc tggtcttcgc gcccatcatg aggtggcgcc
     5341 gaaggcttgc ccttcgttcg accttaagcg ttggtgggag aagaacgaac tggtcacttc
     5401 tgaccgtgga taattaattg aactcactaa agggagacca cag
//
