pHBT-GFP-NOS vector (V012809#)

Price Information

Cat No. Plasmid Name Availability Add to cart
V012809 pHBT-GFP-NOS In stock (lyophilized plasmid)

Buy one, get one free!

Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

pHBT-GFP-NOS vector contained an synthetic green fuorescent protein (GFP) driven by the constitutive 35S enhancer fused to the maize C4PPDK basal promoter.

      • Vector Name:
      • pHBT-GFP-NOS
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 4281 bp
      • Type:
      • Plant Vectors
      • Selection Marker:
      • EGFP
      • Copy Number:
      • High copy number
      • Promoter:
      • 35SPPDK promoter
      • Cloning Method:
      • Enzyme Cut
      • 5' Primer:
      • M13 fwd
      • 3' Primer:
      • M13 rev
      • Fusion Tag:
      • EGFP

pHBT-GFP-NOS vector Vector Map

pHBT-GFP-NOS4281 bp600120018002400300036004200M13 revlac operatorlac promoterCAP binding siteoriAmpRAmpR promoterM13 fwd35SPPDK promoterMCSEGFPNOS terminator

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pHBT-GFP-NOS vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                4281 bp ds-DNA     circular SYN 10-MAR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pHBT-GFP-NOS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4281)
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(39..55)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    63..79
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(87..117)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    132..153
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(441..1029)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1200..2060)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2061..2165)
                     /gene="bla"
                     /note="AmpR promoter"
     primer_bind     2639..2655
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2722..3231
                     /note="35SPPDK promoter"
                     /note="hybrid promoter consisting of the cauliflower mosaic
                     virus 35S enhancer fused to the maize C4PPDK basal promoter
                     (Yoo et al., 2007)"
     misc_feature    3238..3290
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             3291..4010
                     /codon_start=1
                     /product="enhanced GFP"
                     /note="EGFP"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     terminator      4029..4281
                     /note="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"