LOCUS pHBT-GFP-NOS. 4281 bp DNA circular SYN 10-MAR-2023 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS pHBT-GFP-NOS. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4281) AUTHORS caoheibi TITLE Direct Submission FEATURES Location/Qualifiers source 1..4281 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind complement(39..55) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(63..79) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(87..117) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(132..153) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(441..1029) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1203..2060) /label=AmpR /note="beta-lactamase" promoter complement(2061..2165) /label=AmpR promoter primer_bind 2639..2655 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 2722..3231 /label=35SPPDK promoter /note="hybrid promoter consisting of the cauliflower mosaic virus 35S enhancer fused to the maize C4PPDK basal promoter (Yoo et al., 2007)" misc_feature 3234..3290 /label=MCS /note="multiple cloning site" CDS 3291..4007 /label=EGFP /note="enhanced GFP" terminator 4029..4281 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" ORIGIN 1 cgatgataag ctgtcaaaca tgagaattcg taatcatggt catagctgtt tcctgtgtga 61 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc 121 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc 181 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc 241 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 301 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 361 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 421 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 481 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 541 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 601 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 661 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 721 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 781 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 841 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 901 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 961 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 1021 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 1081 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 1141 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 1201 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 1261 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 1321 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 1381 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 1441 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 1501 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 1561 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 1621 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 1681 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 1741 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 1801 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 1861 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 1921 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 1981 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 2041 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 2101 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 2161 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca 2221 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac 2281 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat 2341 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg 2401 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata 2461 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc 2521 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg 2581 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt 2641 aaaacgacgg ccagtgccaa gctctcgaga agcttactcc aagaatatca aagatacagt 2701 ctcagaagac caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct 2761 cggattccat tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg 2821 cacctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga 2881 cagtggtccc aaagatggac ccccacccac aaggagcatc gtggaaaaag aagacgttcc 2941 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc 3001 acaatcccac tatccttcgc cccaagcttg ggcccaagct tgggtcgcgc cccacggatg 3061 gtataagaat aaaggcattc cgcgtgcagg attcacccgt tcgcctctca ccttttcgct 3121 gtactctctc gccacacaca ccccctctcc agctccgttg gagctccgga cagcagcagg 3181 cgcggggcgg tcacgtagta agcagctctc ggctccctct ccccttgctc cgtggatctc 3241 gagctcaagc ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc atggtgagca 3301 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa 3361 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga 3421 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca 3481 ccttcaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact 3541 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg 3601 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca 3661 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt 3721 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg 3781 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc 3841 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca 3901 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt 3961 tcgtgaccgc cgccgggatc actcacggca tggacgagct gtacaagtaa agcggccgcc 4021 cggctgcaga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg 4081 gtcttgcgat gattatcata tatatttctg ttgattacgt taagcatgta ataattaaca 4141 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca 4201 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg 4261 tgtcatctat gttactagat c //