pMV261 vector (V012795)

Price Information

Cat No.	Plasmid Name	Availability	Add to cart
V012795	pMV261	In stock (lyophilized plasmid)	Buy one, get one free!

Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

Plasmid DNA pMV261 is a replicative vector (multicopy, extrachromosomal) that contains a DNA cassette encoding kanamycin resistance (Tn903-derived aph gene), an E. coli origin of replication (oriE), a mycobacterial plasmid DNA origin of replication (oriM), an expression cassette containing a mycobacterial promoter, a multiple cloning site, and a transcriptional terminator.

Vector Name:: pMV261

Antibiotic Resistance:: Kanamycin

Length:: 4488 bp

Type:: Mycobacterium Expression Vector

Replication origin:: ori

Source/Author:: Stover et al., 1991 Nature 351:456

Copy Number:: High copy number

Promoter:: Hsp60

Cloning Method:: Enzyme Cut

pMV261 vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pMV261 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pMV261.        4488 bp DNA     circular SYN 15-DEC-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pMV261.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4488)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4488
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             121..933
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1268..1856
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      2015..3910
                     /label=Mycobacterial OriM region derived from pAL5000
                     /note="Mycobacterial OriM region derived from pAL5000"
     terminator      4406..4452
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"