LOCUS pMV261. 4488 bp DNA circular SYN 15-DEC-2021 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS pMV261. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4488) AUTHORS caoheibi TITLE Direct Submission REFERENCE 2 (bases 1 to 4488) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..4488 /mol_type="other DNA" /organism="synthetic DNA construct" CDS 121..933 /label=KanR /note="aminoglycoside phosphotransferase" rep_origin 1268..1856 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" rep_origin 2015..3910 /label=Mycobacterial OriM region derived from pAL5000 /note="Mycobacterial OriM region derived from pAL5000" terminator 4406..4452 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" ORIGIN 1 gctagccaac aaagcgacgt tgtgtctcaa aatctctgat gttacattgc acaagataaa 61 aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt 121 atgagccata ttcaacggga aacgtcttgc tcgaggccgc gattaaattc caacatggat 181 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc 241 tatcgcttgt atgggaagcc ccatgcgcca gagttgtttc tgaaacatgg caaaggtagc 301 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct 361 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg 421 atccccggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt 481 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct 541 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg 601 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa 661 gaaatgcata atcttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca 721 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc 781 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct 841 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa 901 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa 961 cactggcaga gcattacgct gacttgacgg gacggcggct ttgttgaata aatcgaactt 1021 ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa cgcagaccgt tccgtggcaa 1081 agcaaaagtt caaaatcacc aactggtcca cctacaacaa agctctcatc aaccgtggct 1141 ccctcacttt ctggctggat gatggggcga ttcaggcctg gtatgagtca gcaacacctt 1201 cttcacgagg cagacctcac tagttccact gagcgtcaga ccccgtagaa aagatcaaag 1261 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 1321 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 1381 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc 1441 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 1501 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 1561 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 1621 gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc 1681 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 1741 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 1801 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 1861 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 1921 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata 1981 ccgctcgccg cagccgaacg accgagcgca acgcgtgagc ccaccagctc cgtaagttcg 2041 ggtgctgtgt ggctcgtacc cgcgcattca ggcggcaggg ggtctaacgg gtctaaggcg 2101 gcgtgtacgg ccgccacagc ggctcttagc ggcccggaaa cgtcctcgaa acgacgcatg 2161 tgttcctcct ggttggtaca ggtggttggg ggtgctcggc tgtcgctggt gtttcatcat 2221 cagggctcga cgggagagcg ggggagtgtg cagttgtggg gtggcccctc agcgaaatat 2281 ctgacttgga gctcgtgtcg gaccatacac cggtgattaa tcgtggttta ttatcaagcg 2341 tgagccacgt cgccgacgaa tttgagcagc tctggctgcc gtactggtcc ctggcaagcg 2401 acgatctgct cgaggggatc taccgccaaa gccgcgcgtc ggccctaggc cgccggtaca 2461 tcgaggcgaa cccaacagcg ctggcaaacc tgctggtcgt ggacgtagac catccagacg 2521 cagcgctccg agcgctcagc gcccgggggt cccatccgct gcccaacgcg atcgtgggca 2581 atcgcgccaa cggccacgca cacgcagtgt gggcactcaa cgcccctgtt ccacgcaccg 2641 aatacgcgcg gcgtaagccg ctcgcataca tggcggcgtg cgccgaaggc cttcggcgcg 2701 ccgtcgatgg cgaccgcagt tactcaggcc tcatgaccaa aaaccccggc cacatcgcct 2761 gggaaacgga atggctccac tcagatctct acacactcag ccacatcgag gccgagctcg 2821 gcgcgaacat gccaccgccg cgctggcgtc agcagaccac gtacaaagcg gctccgacgc 2881 cgctagggcg gaattgcgca ctgttcgatt ccgtcaggtt gtgggcctat cttcccgccc 2941 tcatgcggat ctacctgccg acccggaacg tggacggact cggccgcgcg atctatgccg 3001 agtgccacgc gcgaaacgcc gaatttccgt gcaacgacgt gtgtcccgga ccgctaccgg 3061 acagcgaggt ccgcgccatc gccaacagca tttggcgttg gatcacaacc aagtcgcgca 3121 tttgggcgga cgggatcgtg gtctacgagg ccacactcag tgcgcgccat gcggccatct 3181 cgcggaaggg cgcagcagcg cgcacggcgg cgagcacagt tgcgcggcgc gcaaagtccg 3241 cgtcagccat ggaggcattg ctatgagcga cggctacagc gacggctaca gcgacggcta 3301 caactggcag ccgactgtcc gcaaaaagcg gcgcgtgacc gccgccgaag gcgctcgaat 3361 caccggacta tccgaacgcc acgtcgtccg gctcgtggcg caggaacgca gcgagtggtt 3421 cgccgagcag gctgcacgcc gcgaacgcat ccgcgcctat cacgacgacg agggccactc 3481 ttggccgcaa acggccaaac atttcgggct gcatctggac accgttaagc gactcggcta 3541 tcgggcgagg aaagagcgtg cggcagaaca ggaagcggct caaaaggccc acaacgaagc 3601 cgacaatcca ccgctgttct aacgcaattg gggagcgggt gtcgcggggg ttccgtgggg 3661 ggttccgttg caacgggtcg gacaggtaaa agtcctggta gacgctagtt ttctggtttg 3721 ggccatgcct gtctcgttgc gtgtttcgtt gcgtccgttt tgaataccag ccagacgaga 3781 cggggttcta cgaatcttgg tcgataccaa gccatttccg ctgaatatcg tggagctcac 3841 cgccagaatc ggtggttgtg gtgatgtacg tggcgaactc cgttgtagtg cttgtggtgg 3901 catccgtggc gcggccgcgg taccagatct ttaaatctag aggtgaccac aacgacgcgc 3961 ccgctttgat cggggacgtc tgcggccgac catttacggg tcttgttgtc gttggcggtc 4021 atgggccgaa catactcacc cggatcggag ggccgaggac aaggtcgaac gaggggcatg 4081 acccggtgcg gggcttcttg cactcggcat aggcgagtgc taagaataac gttggcactc 4141 gcgaccggtg agtcgtaggt cgggacggtg aggccaggcc cgtcgtcgca gcgagtggca 4201 gcgaggacaa cttgagccgt ccgtcgcggg cactgcgccc ggccagcgta agtagcgggg 4261 ttgccgtcac ccggtgaccc ccggtttcat ccccgatccg gaggaatcac ttcgcaatgg 4321 ccaagacaat tgcggatcca gctgcagaat tcgaagctta tcgatgtcga cgtagttaac 4381 tagcgtacga tcgactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 4441 ctttcgtttt atctgttgtt tgtccggcca tcatggccgc ggtgatca //