LOCUS       pMV261.        4488 bp DNA     circular SYN 15-DEC-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pMV261
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4488
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             121..933
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1268..1856
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      2015..3910
                     /label=Mycobacterial OriM region derived from pAL5000
                     /note="Mycobacterial OriM region derived from pAL5000"
     terminator      4406..4452
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 gctagccaac aaagcgacgt tgtgtctcaa aatctctgat gttacattgc acaagataaa
       61 aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt
      121 atgagccata ttcaacggga aacgtcttgc tcgaggccgc gattaaattc caacatggat
      181 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
      241 tatcgcttgt atgggaagcc ccatgcgcca gagttgtttc tgaaacatgg caaaggtagc
      301 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
      361 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
      421 atccccggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
      481 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
      541 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
      601 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
      661 gaaatgcata atcttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
      721 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
      781 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
      841 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
      901 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
      961 cactggcaga gcattacgct gacttgacgg gacggcggct ttgttgaata aatcgaactt
     1021 ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa cgcagaccgt tccgtggcaa
     1081 agcaaaagtt caaaatcacc aactggtcca cctacaacaa agctctcatc aaccgtggct
     1141 ccctcacttt ctggctggat gatggggcga ttcaggcctg gtatgagtca gcaacacctt
     1201 cttcacgagg cagacctcac tagttccact gagcgtcaga ccccgtagaa aagatcaaag
     1261 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1321 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1381 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     1441 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1501 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1561 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1621 gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc
     1681 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1741 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1801 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     1861 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     1921 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     1981 ccgctcgccg cagccgaacg accgagcgca acgcgtgagc ccaccagctc cgtaagttcg
     2041 ggtgctgtgt ggctcgtacc cgcgcattca ggcggcaggg ggtctaacgg gtctaaggcg
     2101 gcgtgtacgg ccgccacagc ggctcttagc ggcccggaaa cgtcctcgaa acgacgcatg
     2161 tgttcctcct ggttggtaca ggtggttggg ggtgctcggc tgtcgctggt gtttcatcat
     2221 cagggctcga cgggagagcg ggggagtgtg cagttgtggg gtggcccctc agcgaaatat
     2281 ctgacttgga gctcgtgtcg gaccatacac cggtgattaa tcgtggttta ttatcaagcg
     2341 tgagccacgt cgccgacgaa tttgagcagc tctggctgcc gtactggtcc ctggcaagcg
     2401 acgatctgct cgaggggatc taccgccaaa gccgcgcgtc ggccctaggc cgccggtaca
     2461 tcgaggcgaa cccaacagcg ctggcaaacc tgctggtcgt ggacgtagac catccagacg
     2521 cagcgctccg agcgctcagc gcccgggggt cccatccgct gcccaacgcg atcgtgggca
     2581 atcgcgccaa cggccacgca cacgcagtgt gggcactcaa cgcccctgtt ccacgcaccg
     2641 aatacgcgcg gcgtaagccg ctcgcataca tggcggcgtg cgccgaaggc cttcggcgcg
     2701 ccgtcgatgg cgaccgcagt tactcaggcc tcatgaccaa aaaccccggc cacatcgcct
     2761 gggaaacgga atggctccac tcagatctct acacactcag ccacatcgag gccgagctcg
     2821 gcgcgaacat gccaccgccg cgctggcgtc agcagaccac gtacaaagcg gctccgacgc
     2881 cgctagggcg gaattgcgca ctgttcgatt ccgtcaggtt gtgggcctat cttcccgccc
     2941 tcatgcggat ctacctgccg acccggaacg tggacggact cggccgcgcg atctatgccg
     3001 agtgccacgc gcgaaacgcc gaatttccgt gcaacgacgt gtgtcccgga ccgctaccgg
     3061 acagcgaggt ccgcgccatc gccaacagca tttggcgttg gatcacaacc aagtcgcgca
     3121 tttgggcgga cgggatcgtg gtctacgagg ccacactcag tgcgcgccat gcggccatct
     3181 cgcggaaggg cgcagcagcg cgcacggcgg cgagcacagt tgcgcggcgc gcaaagtccg
     3241 cgtcagccat ggaggcattg ctatgagcga cggctacagc gacggctaca gcgacggcta
     3301 caactggcag ccgactgtcc gcaaaaagcg gcgcgtgacc gccgccgaag gcgctcgaat
     3361 caccggacta tccgaacgcc acgtcgtccg gctcgtggcg caggaacgca gcgagtggtt
     3421 cgccgagcag gctgcacgcc gcgaacgcat ccgcgcctat cacgacgacg agggccactc
     3481 ttggccgcaa acggccaaac atttcgggct gcatctggac accgttaagc gactcggcta
     3541 tcgggcgagg aaagagcgtg cggcagaaca ggaagcggct caaaaggccc acaacgaagc
     3601 cgacaatcca ccgctgttct aacgcaattg gggagcgggt gtcgcggggg ttccgtgggg
     3661 ggttccgttg caacgggtcg gacaggtaaa agtcctggta gacgctagtt ttctggtttg
     3721 ggccatgcct gtctcgttgc gtgtttcgtt gcgtccgttt tgaataccag ccagacgaga
     3781 cggggttcta cgaatcttgg tcgataccaa gccatttccg ctgaatatcg tggagctcac
     3841 cgccagaatc ggtggttgtg gtgatgtacg tggcgaactc cgttgtagtg cttgtggtgg
     3901 catccgtggc gcggccgcgg taccagatct ttaaatctag aggtgaccac aacgacgcgc
     3961 ccgctttgat cggggacgtc tgcggccgac catttacggg tcttgttgtc gttggcggtc
     4021 atgggccgaa catactcacc cggatcggag ggccgaggac aaggtcgaac gaggggcatg
     4081 acccggtgcg gggcttcttg cactcggcat aggcgagtgc taagaataac gttggcactc
     4141 gcgaccggtg agtcgtaggt cgggacggtg aggccaggcc cgtcgtcgca gcgagtggca
     4201 gcgaggacaa cttgagccgt ccgtcgcggg cactgcgccc ggccagcgta agtagcgggg
     4261 ttgccgtcac ccggtgaccc ccggtttcat ccccgatccg gaggaatcac ttcgcaatgg
     4321 ccaagacaat tgcggatcca gctgcagaat tcgaagctta tcgatgtcga cgtagttaac
     4381 tagcgtacga tcgactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     4441 ctttcgtttt atctgttgtt tgtccggcca tcatggccgc ggtgatca
//
