Basic Vector Information
2nd generation lentiviral packaging plasmid. Can be used with 2nd and 3rd generation transfer vectors. Use in conjunction with an envelope plasmid such as pCMV-VSV-G.
- Vector Name:
- pCMV-dR8.2 dvpr
- Antibiotic Resistance:
- Ampicillin
- Length:
- 13380 bp
- Type:
- Viral Expression & Packaging Vectors
- Replication origin:
- ori
- Source/Author:
- Bob Weinberg
- Copy Number:
- High copy number
- Promoter:
- CMV
pCMV-dR8.2 dvpr vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pCMV-dR8.2 dvpr vector Sequence
LOCUS addgene-plasmid- 13380 bp DNA circular SYN 13-DEC-2021 DEFINITION 2nd generation lentiviral packaging plasmid. Can be used with 2nd and 3rd generation transfer vectors. Use in conjunction with an envelope plasmid such as pCMV-VSV-G.. ACCESSION . VERSION . KEYWORDS addgene-plasmid-8455-sequence-327823. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13380) AUTHORS Stewart SA, Dykxhoorn DM, Palliser D, Mizuno H, Yu EY, An DS, Sabatini DM, Chen IS, Hahn WC, Sharp PA, Weinberg RA, Novina CD TITLE Lentivirus-delivered stable gene silencing by RNAi in primary cells. JOURNAL RNA 2003 Apr;9(4):493-501. PUBMED 12649500 REFERENCE 2 (bases 1 to 13380) TITLE Direct Submission REFERENCE 3 (bases 1 to 13380) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "RNA"; date: "2003-04"; volume: "9(4)"; pages: "493-501" COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..13380 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 1..380 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 381..584 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" CDS 855..2354 /label=HIV-1 gag /note="gag protein from human immunodeficiency virus 1" CDS 2150..5158 /label=HIV-1 pol /note="pol protein from human immunodeficiency virus 1" CDS 5106..5681 /gene="vif" /label=vif /note="Virion infectivity factor from Human immunodeficiency virus type 1 group M subtype B (isolate NY5). Accession#: P12504" CDS 5821..5994 /note="Protein Tat from Human immunodeficiency virus type 1 group M subtype B (isolate BH5). Accession#: P04612" CDS 6052..6294 /gene="vpu" /label=vpu /note="Protein Vpu from Human immunodeficiency virus type 1 group M subtype B (isolate BH10). Accession#: P69699" misc_feature 6458..6691 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 6876..6920 /label=gp41 peptide /note="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" CDS 7069..7110 /note="Protein Tat from Human immunodeficiency virus type 1 group M subtype B (isolate WMJ22). Accession#: P12509" CDS 7214..7240 /label=NES /note="nuclear export signal from the HIV Rev protein (Fischer et al., 1995)" CDS 7486..8103 /gene="nef" /label=nef /note="Protein Nef from Human immunodeficiency virus type 1 group M subtype B (isolate LW123). Accession#: Q70627" promoter complement(8477..8495) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(8785..8804) /label=pBRrevBam /note="pBRrevBam" /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" promoter 9283..9387 /label=AmpR promoter CDS 9388..10245 /label=AmpR /note="beta-lactamase" rep_origin 10419..11007 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" promoter 11253..11582 /label=SV40 promoter /note="SV40 enhancer and early promoter" intron 12716..12781 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 12911..12931 /label=SV40 NLS /note="nuclear localization signal of SV40 (simian virus 40) large T antigen"
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