LOCUS       addgene-plasmid-       13380 bp DNA     circular SYN 13-DEC-2021
DEFINITION  2nd generation lentiviral packaging plasmid. Can be used with 2nd 
            and 3rd generation transfer vectors. Use in conjunction with an 
            envelope plasmid such as pCMV-VSV-G..
ACCESSION   .
VERSION     .
KEYWORDS    addgene-plasmid-8455-sequence-327823.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13380)
  AUTHORS   Stewart SA, Dykxhoorn DM, Palliser D, Mizuno H, Yu EY, An DS, 
            Sabatini DM, Chen IS, Hahn WC, Sharp PA, Weinberg RA, Novina CD
  TITLE     Lentivirus-delivered stable gene silencing by RNAi in primary cells.
  JOURNAL   RNA 2003 Apr;9(4):493-501.
  PUBMED    12649500
REFERENCE   2  (bases 1 to 13380)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 13380)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA"; date:
            "2003-04"; volume: "9(4)"; pages: "493-501"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13380
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             855..2354
                     /label=HIV-1 gag
                     /note="gag protein from human immunodeficiency virus 1"
     CDS             2150..5158
                     /label=HIV-1 pol
                     /note="pol protein from human immunodeficiency virus 1"
     CDS             5106..5681
                     /gene="vif"
                     /label=vif
                     /note="Virion infectivity factor from Human
                     immunodeficiency virus type 1 group M subtype B (isolate 
                     NY5). Accession#: P12504"
     CDS             5821..5994
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate BH5). Accession#: P04612"
     CDS             6052..6294
                     /gene="vpu"
                     /label=vpu
                     /note="Protein Vpu from Human immunodeficiency virus type 1
                     group M subtype B (isolate BH10). Accession#: P69699"
     misc_feature    6458..6691
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6876..6920
                     /label=gp41 peptide
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et 
                     al., 2013)"
     CDS             7069..7110
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     CDS             7214..7240
                     /label=NES
                     /note="nuclear export signal from the HIV Rev protein
                     (Fischer et al., 1995)"
     CDS             7486..8103
                     /gene="nef"
                     /label=nef
                     /note="Protein Nef from Human immunodeficiency virus type 1
                     group M subtype B (isolate LW123). Accession#: Q70627"
     promoter        complement(8477..8495)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8785..8804)
                     /label=pBRrevBam
                     /note="pBRrevBam"
                     /note="pBR322 vectors, tet region, downstream of BamHI,
                     reverse primer"
     promoter        9283..9387
                     /label=AmpR promoter
     CDS             9388..10245
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      10419..11007
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        11253..11582
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     intron          12716..12781
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             12911..12931
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg
      721 taagtaccgc ctatagagtc tataggccca cccccttggc ttcttatgcg acggatcgat
      781 cccgtaataa gcttcgaggt ccgcggccgc gttgacgcgc acggcaagag gcgaggggcg
      841 gcgactggtg agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgat
      901 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      961 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     1021 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     1081 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     1141 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag aaaaaagcac
     1201 agcaagcagc agctgacaca ggacacagca atcaggtcag ccaaaattac cctatagtgc
     1261 agaacatcca ggggcaaatg gtacatcagg ccatatcacc tagaacttta aatgcatggg
     1321 taaaagtagt agaagagaag gctttcagcc cagaagtgat acccatgttt tcagcattat
     1381 cagaaggagc caccccacaa gatttaaaca ccatgctaaa cacagtgggg ggacatcaag
     1441 cagccatgca aatgttaaaa gagaccatca atgaggaagc tgcagaatgg gatagagtgc
     1501 atccagtgca tgcagggcct attgcaccag gccagatgag agaaccaagg ggaagtgaca
     1561 tagcaggaac tactagtacc cttcaggaac aaataggatg gatgacacat aatccaccta
     1621 tcccagtagg agaaatctat aaaagatgga taatcctggg attaaataaa atagtaagaa
     1681 tgtatagccc taccagcatt ctggacataa gacaaggacc aaaggaaccc tttagagact
     1741 atgtagaccg attctataaa actctaagag ccgagcaagc ttcacaagag gtaaaaaatt
     1801 ggatgacaga aaccttgttg gtccaaaatg cgaacccaga ttgtaagact attttaaaag
     1861 cattgggacc aggagcgaca ctagaagaaa tgatgacagc atgtcaggga gtggggggac
     1921 ccggccataa agcaagagtt ttggctgaag caatgagcca agtaacaaat ccagctacca
     1981 taatgataca gaaaggcaat tttaggaacc aaagaaagac tgttaagtgt ttcaattgtg
     2041 gcaaagaagg gcacatagcc aaaaattgca gggcccctag gaaaaagggc tgttggaaat
     2101 gtggaaagga aggacaccaa atgaaagatt gtactgagag acaggctaat tttttaggga
     2161 agatctggcc ttcccacaag ggaaggccag ggaattttct tcagagcaga ccagagccaa
     2221 cagccccacc agaagagagc ttcaggtttg gggaagagac aacaactccc tctcagaagc
     2281 aggagccgat agacaaggaa ctgtatcctt tagcttccct cagatcactc tttggcagcg
     2341 acccctcgtc acaataaaga taggggggca attaaaggaa gctctattag atacaggagc
     2401 agatgataca gtattagaag aaatgaattt gccaggaaga tggaaaccaa aaatgatagg
     2461 gggaattgga ggttttatca aagtaagaca gtatgatcag atactcatag aaatctgcgg
     2521 acataaagct ataggtacag tattagtagg acctacacct gtcaacataa ttggaagaaa
     2581 tctgttgact cagattggct gcactttaaa ttttcccatt agtcctattg agactgtacc
     2641 agtaaaatta aagccaggaa tggatggccc aaaagttaaa caatggccat tgacagaaga
     2701 aaaaataaaa gcattagtag aaatttgtac agaaatggaa aaggaaggaa aaatttcaaa
     2761 aattgggcct gaaaatccat acaatactcc agtatttgcc ataaagaaaa aagacagtac
     2821 taaatggaga aaattagtag atttcagaga acttaataag agaactcaag atttctggga
     2881 agttcaatta ggaataccac atcctgcagg gttaaaacag aaaaaatcag taacagtact
     2941 ggatgtgggc gatgcatatt tttcagttcc cttagataaa gacttcagga agtatactgc
     3001 atttaccata cctagtataa acaatgagac accagggatt agatatcagt acaatgtgct
     3061 tccacaggga tggaaaggat caccagcaat attccagtgt agcatgacaa aaatcttaga
     3121 gccttttaga aaacaaaatc cagacatagt catctatcaa tacatggatg atttgtatgt
     3181 aggatctgac ttagaaatag ggcagcatag aacaaaaata gaggaactga gacaacatct
     3241 gttgaggtgg ggatttacca caccagacaa aaaacatcag aaagaacctc cattcctttg
     3301 gatgggttat gaactccatc ctgataaatg gacagtacag cctatagtgc tgccagaaaa
     3361 ggacagctgg actgtcaatg acatacagaa attagtggga aaattgaatt gggcaagtca
     3421 gatttatgca gggattaaag taaggcaatt atgtaaactt cttaggggaa ccaaagcact
     3481 aacagaagta gtaccactaa cagaagaagc agagctagaa ctggcagaaa acagggagat
     3541 tctaaaagaa ccggtacatg gagtgtatta tgacccatca aaagacttaa tagcagaaat
     3601 acagaagcag gggcaaggcc aatggacata tcaaatttat caagagccat ttaaaaatct
     3661 gaaaacagga aagtatgcaa gaatgaaggg tgcccacact aatgatgtga aacaattaac
     3721 agaggcagta caaaaaatag ccacagaaag catagtaata tggggaaaga ctcctaaatt
     3781 taaattaccc atacaaaagg aaacatggga agcatggtgg acagagtatt ggcaagccac
     3841 ctggattcct gagtgggagt ttgtcaatac ccctccctta gtgaagttat ggtaccagtt
     3901 agagaaagaa cccataatag gagcagaaac tttctatgta gatggggcag ccaataggga
     3961 aactaaatta ggaaaagcag gatatgtaac tgacagagga agacaaaaag ttgtccccct
     4021 aacggacaca acaaatcaga agactgagtt acaagcaatt catctagctt tgcaggattc
     4081 gggattagaa gtaaacatag tgacagactc acaatatgca ttgggaatca ttcaagcaca
     4141 accagataag agtgaatcag agttagtcag tcaaataata gagcagttaa taaaaaagga
     4201 aaaagtctac ctggcatggg taccagcaca caaaggaatt ggaggaaatg aacaagtaga
     4261 taaattggtc agtgctggaa tcaggaaagt actattttta gatggaatag ataaggccca
     4321 agaagaacat gagaaatatc acagtaattg gagagcaatg gctagtgatt ttaacctacc
     4381 acctgtagta gcaaaagaaa tagtagccag ctgtgataaa tgtcagctaa aaggggaagc
     4441 catgcatgga caagtagact gtagcccagg aatatggcag ctagattgta cacatttaga
     4501 aggaaaagtt atcttggtag cagttcatgt agccagtgga tatatagaag cagaagtaat
     4561 tccagcagag acagggcaag aaacagcata cttcctctta aaattagcag gaagatggcc
     4621 agtaaaaaca gtacatacag acaatggcag caatttcacc agtactacag ttaaggccgc
     4681 ctgttggtgg gcggggatca agcaggaatt tggcattccc tacaatcccc aaagtcaagg
     4741 agtaatagaa tctatgaata aagaattaaa gaaaattata ggacaggtaa gagatcaggc
     4801 tgaacatctt aagacagcag tacaaatggc agtattcatc cacaatttta aaagaaaagg
     4861 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     4921 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     4981 cagcagagat ccagtttgga aaggaccagc aaagctcctc tggaaaggtg aaggggcagt
     5041 agtaatacaa gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcatcag
     5101 ggattatgga aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta
     5161 acacatggaa aagattagta aaacaccata tgtatatttc aaggaaagct aaggactggt
     5221 tttatagaca tcactatgaa agtactaatc caaaaataag ttcagaagta cacatcccac
     5281 taggggatgc taaattagta ataacaacat attggggtct gcatacagga gaaagagact
     5341 ggcatttggg tcagggagtc tccatagaat ggaggaaaaa gagatatagc acacaagtag
     5401 accctgacct agcagaccaa ctaattcatc tgcactattt tgattgtttt tcagaatctg
     5461 ctataagaaa taccatatta ggacgtatag ttagtcctag gtgtgaatat caagcaggac
     5521 ataacaaggt aggatctcta cagtacttgg cactagcagc attaataaaa ccaaaacaga
     5581 taaagccacc tttgcctagt gttaggaaac tgacagagga cagatggaac aagccccaga
     5641 agaccaaggg ccacagaggg agccatacaa tgaatggaca ctagagcttt agtagcccga
     5701 acccggggcg gaccgaagtg gaagccataa taagaattct gcaacaactg ctgtttatcc
     5761 atttcagaat tgggtgtcga catagcagaa taggcgttac tcgacagagg agagcaagaa
     5821 atggagccag tagatcctag actagagccc tggaagcatc caggaagtca gcctaaaact
     5881 gcttgtacca attgctattg taaaaagtgt tgctttcatt gccaagtttg tttcatgaca
     5941 aaagccttag gcatctccta tggcaggaag aagcggagac agcgacgaag agctcatcag
     6001 aacagtcaga ctcatcaagc ttctctatca aagcagtaag tagtacatgt aatgcaacct
     6061 ataatagtag caatagtagc attagtagta gcaataataa tagcaatagt tgtgtggtcc
     6121 atagtaatca tagaatatag gaaaatatta agacaaagaa aaatagacag gttaattgat
     6181 agactaatag aaagagcaga agacagtggc aatgagagtg aaggagaagt atcagcactt
     6241 gtggagatgg gggtggaaat ggggcaccat gctccttggg atattgatga tctgtagttg
     6301 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     6361 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     6421 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     6481 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     6541 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     6601 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     6661 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     6721 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     6781 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     6841 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     6901 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     6961 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     7021 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     7081 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
     7141 acagatccat tcgattagtg aacggatcct tggcacttat ctgggacgat ctgcggagcc
     7201 tgtgcctctt cagctaccac cgcttgagag acttactctt gattgtaacg aggattgtgg
     7261 aacttctggg acgcaggggg tgggaagccc tcaaatattg gtggaatctc ctacaatatt
     7321 ggagtcagga gctaaagaat agtgctgtta gcttgctcaa tgccacagcc atagcagtag
     7381 ctgaggggac agatagggtt atagaagtag tacaaggagc ttgtagagct attcgccaca
     7441 tacctagaag aataagacag ggcttggaaa ggattttgct ataagatggg tggcaagtgg
     7501 tcaaaaagta gtgtgattgg atggcctact gtaagggaaa gaatgagacg agctgagcca
     7561 gcagcagatg gggtgggagc agtatctcga gacctagaaa aacatggagc aatcacaagt
     7621 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag
     7681 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta
     7741 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     7801 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag
     7861 aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta
     7921 gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta
     7981 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt
     8041 gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggagta cttcaagaac
     8101 tgctgagcgg ccgccccggt gaccttcaga ccttggcact ggaggtggcc cggcagaagc
     8161 gcggcatcgt ggatcagtgc tgcaccagca tctgctctct ctaccaactg gagaactact
     8221 gcaactaggc ccaccactac cctgtccacc cctctgcaat gaataaaacc tttgaaagag
     8281 cactacaagt tgtgtgtaca tgcgtgcatg tgcatatgtg gtgcgggggg aacatgagtg
     8341 gggctggctg gagtggcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg
     8401 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagtctag
     8461 aattaattcc gtgtattcta tagtgtcacc taaatcgtat gtgtatgata cataaggtta
     8521 tgtattaatt gtagccgcgt tctaacgaca atatgtacaa gcctaattgt gtagcatctg
     8581 gcttactgaa gcagacccta tcatctctct cgtaaactgc cgtcagagtc ggtttggttg
     8641 gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc agcgaaccaa
     8701 tcagcagggt catcgctagc cagatcctct acgccggacg catcgtggcc ggcatcaccg
     8761 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg
     8821 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg
     8881 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca
     8941 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc
     9001 gaatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     9061 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     9121 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     9181 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     9241 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     9301 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     9361 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     9421 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     9481 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     9541 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     9601 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     9661 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     9721 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     9781 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     9841 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     9901 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     9961 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
    10021 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
    10081 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
    10141 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
    10201 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
    10261 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
    10321 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
    10381 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
    10441 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
    10501 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
    10561 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
    10621 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
    10681 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
    10741 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
    10801 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
    10861 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
    10921 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
    10981 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
    11041 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
    11101 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
    11161 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
    11221 cgttggccga ttcattaatg cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc
    11281 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
    11341 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
    11401 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
    11461 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
    11521 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
    11581 aaaagcttgg acacaagaca ggcttgcgag atatgtttga gaataccact ttatcccgcg
    11641 tcagggagag gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg
    11701 ccagatctct ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat
    11761 aaacaggctg ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag
    11821 atccatgcac gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt
    11881 gccgtaagcc gtggcggtct gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc
    11941 atgtcgatac cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc
    12001 tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg
    12061 gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg
    12121 caaaaccggc tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct
    12181 ggattgaaca gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat
    12241 cttttcaacg cctggcactg ccgggcgttg ttctttttaa cttcaggcgg gttacaatag
    12301 tttccagtaa gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg
    12361 ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc
    12421 gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg gtatcgcatg
    12481 attaaccgtc tgatgtggat ctggcgcggc attgacccac gcgaaatcct cgacgtccag
    12541 gcacgtattg tgatgagcga tgccgaacgt accgacgatg atttatacga tacggtgatt
    12601 ggctaccgtg gcggcaactg gatttatgag tgggccccgg atctttgtga aggaacctta
    12661 cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa
    12721 tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta
    12781 gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa
    12841 cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca
    12901 ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt
    12961 gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta
    13021 caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac
    13081 ctttataagt aggcataaca gttataatca taacatactg ttttttctta ctccacacag
    13141 gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat
    13201 ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca
    13261 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
    13321 acctgaaaca taaaatgaat gcaattgttg ttgttgggct gcaggaattc gagctcgccc
//
