pRSV-Rev vector (V012790)

Price Information

Cat No.	Plasmid Name	Availability	Add to cart
V012790	pRSV-Rev	In stock (lyophilized plasmid)	Buy one, get one free!

Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE and envelope expressing plasmid.

Vector Name:: pRSV-Rev

Antibiotic Resistance:: Ampicillin

Length:: 4174 bp

Type:: Viral Expression &amp; Packaging Vectors

Replication origin:: ori

Source/Author:: Didier Trono

Copy Number:: High copy number

Promoter:: RSV

Growth Strain(s):: JM108

Growth Temperature:: 37℃

pRSV-Rev vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

References

Farzaneh M, Sayyah M, Eshraghi HR, Panahi N, Mirzapourdelavar H, Gholami Pourbadie H. The Lentiviral Vector Pseudotyped by Modified Rabies Glycoprotein Does Not Cause Reactive Gliosis and Neurodegeneration in Rat Hippocampus. Iran Biomed J. 2019 Sep;23(5):324-9. doi: 10.29252/.23.5.324. Epub 2019 May 19. PMID: 31103020; PMCID: PMC6661131.

pRSV-Rev vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_38283        4174 bp DNA     circular SYN 13-DEC-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4174)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4174
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        365..627
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     CDS             756..1103
                     /codon_start=1
                     /label=Rev
                     /note="human immunodeficiency virus 1 (HIV-1) Rev protein"
                     /translation="MAGRSGDSDEDLLKAVRLIKFLYQSNPPPNPEGTRQARRNRRRRW
                     RERQRQIHSISERILSTYLGRSAEPVPLQLPPLERLTLDCNEDCGTSGTQGVGSPQILV
                     ESPTILESGAKE"
     primer_bind     complement(1190..1206)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1532..1987
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2269..2373
                     /label=AmpR promoter
     CDS             2374..3231
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3405..3993
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"