LOCUS       40924_38283        4174 bp DNA     circular SYN 13-DEC-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4174
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        365..627
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     CDS             756..1103
                     /codon_start=1
                     /label=Rev
                     /note="human immunodeficiency virus 1 (HIV-1) Rev protein"
                     /translation="MAGRSGDSDEDLLKAVRLIKFLYQSNPPPNPEGTRQARRNRRRRW
                     RERQRQIHSISERILSTYLGRSAEPVPLQLPPLERLTLDCNEDCGTSGTQGVGSPQILV
                     ESPTILESGAKE"
     primer_bind     complement(1190..1206)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1532..1987
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2269..2373
                     /label=AmpR promoter
     CDS             2374..3231
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3405..3993
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gaattcgatg
      241 tacgggccag atatacgcgt atctgagggg actaggtgtg tttaggcgaa aagcggggct
      301 tcggttgtac gcggttagga gtcccctcag gattagtagt ttcgcttttg catagggagg
      361 gggaaatgta gtcttatgca atacacttgt agtcttgcaa catggtaacg atgagttagc
      421 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt
      481 acgatcgtgc cttattagga aggcaacaga caggtctgac atggattgga cgaaccactg
      541 aattccgcat tgcagagata attgtattta agtgcctagc tcgatacaat aaacgccatt
      601 tgaccattca ccacattggt gtgcacctcc aagctcgagc tcgtttagtg aaccgtcaga
      661 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
      721 gcctcccctc gaagctagtc gattaggcat ctcctatggc aggaagaagc ggagacagcg
      781 acgaagacct cctcaaggca gtcagactca tcaagtttct ctatcaaagc aacccacctc
      841 ccaatcccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
      901 agagacagat ccattcgatt agtgaacgga tccttagcac ttatctggga cgatctgcgg
      961 agcctgtgcc tcttcagcta ccaccgcttg agagacttac tcttgattgt aacgaggatt
     1021 gtggaacttc tgggacgcag ggggtgggaa gccctcaaat attggtggaa tctcctacaa
     1081 tattggagtc aggagctaaa gaatagtgct gttagcttgc tcaatgccac agctatagca
     1141 gtagctgagg ggacagatag ggttatagaa gtagtacaag aagcttggca ctggccgtcg
     1201 ttttacatga tctgagcctg ggagatctct ggctaactag ggaacccact gcttaagcct
     1261 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     1321 aactagagat caggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     1381 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     1441 cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     1501 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg
     1561 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     1621 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     1681 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     1741 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     1801 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     1861 aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     1921 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
     1981 acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     2041 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     2101 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     2161 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     2221 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     2281 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     2341 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     2401 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     2461 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     2521 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     2581 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     2641 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     2701 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     2761 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     2821 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     2881 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     2941 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     3001 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     3061 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     3121 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     3181 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     3241 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     3301 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     3361 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     3421 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     3481 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     3541 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     3601 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     3661 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     3721 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     3781 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     3841 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     3901 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     3961 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     4021 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     4081 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     4141 gagcgcagcg agtcagtgag cgaggaagcg gaag
//
