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pAAV-hSyn-GRAB_DA1h vector (V012732#)

Basic Vector Information

express the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1h in neurons

Basic Vector Information
Vector Name pAAV-hSyn-GRAB_DA1h Antibiotic Resistance Ampicillin
Length 6349 bp Type Viral Expression & Packaging Vectors
Source Yulong Li Copy Number High copy number
Promoter human synapsin Expression Method Constiutive, Stable

pAAV-hSyn-GRAB_DA1h vector Vector Map

pAAV-hSyn-GRAB_DA1h vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                6349 bp ds-DNA     circular UNA 09-APR-2021
DEFINITION  natural circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-hSyn-GRAB_DA1h
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6349)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6349
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     repeat_region   1..141
                     /note="AAV2 ITR"
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     promoter        156..597
                     /note="synapsin"
     CDS             598..660
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /note="Ig-kappa leader"
                     /translation="METDTLLLWVLLLWVPGSTGD"
     protein_bind    661..685
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /note="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             694..1446
                     /codon_start=1
                     /note="D(2) dopamine receptor(2-252)"
                     /translation="DPLNLSWYDDDLERQNWSRPFNGSDGKADRPHYNYYATLLTLLIA
                     VIVFGNVLVCMAVSREKALQTTTNYLIVSLAVADLLVATLVMPWVVYLEVVGEWKFSRI
                     HCDIFVTLDVMMCTASILNLCAISIDRYTAVAMPMLYNTRYSSKRRVTVMISIVWVLSF
                     TISCPLLFGLNNADQNECIIANPAFVVYSSIVSFYVPFIVMLLVYIKIYIVLRRRRKRV
                     NTKRSSRAFRAHLRAPLKGNCTHPEDMKL"
     CDS             1453..2175
                     /codon_start=1
                     /note="circular-permutated EGFP(cpEGFP)"
                     /translation="NVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGDGPV
                     LLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEEL
                     FTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTY
                     GVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIEL
                     KGIDFKEDGNILGHKLEYN"
     CDS             2191..2451
                     /codon_start=1
                     /note="D(2) dopamine receptor(358-443)"
                     /translation="MSRRKLSQQKEKKATQMLAIVLGVFIICWLPFFITHILNIHCDCN
                     IPPVLYSAFTWLGYVNSAVNPIIYTTFNIEFRKAFLKILHC"
     unsure          2466..3074
                     /note="WPRE"
     polyA_signal    3094..3572
                     /label=hGH polyA
                     /note="PolyA signal"
     repeat_region   3612..3752
                     /note="AAV2 ITR"
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      3827..4282
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4564..4668
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             4669..5529
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5677..6344
                     /label=pUC ori
                     /note="Replication origin"

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