LOCUS pAAV-hSyn-GRAB_D 6349 bp DNA circular SYN 09-APR-2021 DEFINITION natural circular DNA. ACCESSION . VERSION . KEYWORDS pAAV-hSyn-GRAB_DA1h. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6349) TITLE Direct Submission REFERENCE 2 (bases 1 to 6349) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..6349 /mol_type="other DNA" /organism="synthetic DNA construct" repeat_region 1..141 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" promoter 156..597 /note="synapsin" CDS 598..660 /codon_start=1 /product="leader sequence from mouse immunoglobulin kappa light chain" /label=leader sequence from mouse immunoglobulin kappa /note="Ig-kappa leader" /translation="METDTLLLWVLLLWVPGSTGD" protein_bind 661..685 /gene="mutant version of attB" /label=BP Clonase(TM) binding site /bound_moiety="BP Clonase(TM)" /note="attB1" /note="recombination site for the Gateway(R) BP reaction" CDS 694..1446 /codon_start=1 /label=D(2) dopamine receptor(2-252) /note="D(2) dopamine receptor(2-252)" /translation="DPLNLSWYDDDLERQNWSRPFNGSDGKADRPHYNYYATLLTLLIA VIVFGNVLVCMAVSREKALQTTTNYLIVSLAVADLLVATLVMPWVVYLEVVGEWKFSRI HCDIFVTLDVMMCTASILNLCAISIDRYTAVAMPMLYNTRYSSKRRVTVMISIVWVLSF TISCPLLFGLNNADQNECIIANPAFVVYSSIVSFYVPFIVMLLVYIKIYIVLRRRRKRV NTKRSSRAFRAHLRAPLKGNCTHPEDMKL" CDS 1471..1722 /label=VC155 /note="C-terminal fragment of mVenus for use in bimolecular fluorescence complementation (BiFC) (Kodama and Hu, 2010)" CDS 2191..2451 /codon_start=1 /label=D(2) dopamine receptor(358-443) /note="D(2) dopamine receptor(358-443)" /translation="MSRRKLSQQKEKKATQMLAIVLGVFIICWLPFFITHILNIHCDCN IPPVLYSAFTWLGYVNSAVNPIIYTTFNIEFRKAFLKILHC" misc_feature 2476..3064 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" polyA_signal 3096..3572 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" repeat_region 3612..3752 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" rep_origin 3827..4282 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 4564..4668 /label=AmpR promoter CDS 4669..5526 /label=AmpR /note="beta-lactamase" rep_origin 5700..6288 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtatgag gcggggtggg ggtgcctacc 181 tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc 241 ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt 301 cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca 361 ctgaaggcgc gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg 421 gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg 481 ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg 541 tgggcagcgg aggagtcgtg tcgtgcctga gagcgcagtc gagaaaccgg tgccaccatg 601 gagacagaca cactcctgct atgggtactg ctgctctggg ttccaggttc cactggtgac 661 acaagtttgt acaaaaaagt tggcaccacc ggtgatccac tgaatctgtc ctggtatgat 721 gatgatctgg agaggcagaa ctggagccgg cccttcaacg ggtcagacgg gaaggcggac 781 agaccccact acaactacta tgccacactg ctcaccctgc tcatcgctgt catcgtcttc 841 ggcaacgtgc tggtgtgcat ggctgtgtcc cgcgagaagg cgctgcagac caccaccaac 901 tacctgatcg tcagcctcgc agtggccgac ctcctcgtcg ccacactggt catgccctgg 961 gttgtctacc tggaggtggt aggtgagtgg aaattcagca ggattcactg tgacatcttc 1021 gtcactctgg acgtcatgat gtgcacggcg agcatcctga acttgtgtgc catcagcatc 1081 gacaggtaca cagctgtggc catgcccatg ctgtacaata cgcgctacag ctccaagcgc 1141 cgggtcaccg tcatgatctc catcgtctgg gtcctgtcct tcaccatctc ctgcccactc 1201 ctcttcggac tcaataacgc agaccagaac gagtgcatca ttgccaaccc ggccttcgtg 1261 gtctactcct ccatcgtctc cttctacgtg cccttcattg tcatgctgct ggtctacatc 1321 aagatctaca ttgtcctccg cagacgccgc aagcgagtca acaccaaacg cagcagccga 1381 gctttcaggg cccacctgag ggctccacta aagggcaact gtactcaccc cgaggacatg 1441 aaactcggag gaaacgtcta tatcaaggcc gacaagcaga agaacggcat caaggcgaac 1501 ttccacatcc gccacaacat cgaggacggc ggcgtgcagc tcgcctacca ctaccagcag 1561 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcgtgcag 1621 tccaaacttt cgaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg 1681 accgccgccg ggatcactct cggcatggac gagctgtaca agggcggtac cggagggagc 1741 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac 1801 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gtgagggcga tgccacctac 1861 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc 1921 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag 1981 cagcacgact tcttcaagtc cgccatgccc gaaggctaca tccaggagcg caccatcttc 2041 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg 2101 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac 2161 aagctggagt acaacactgg agcagcagca atgagccgta ggaagctctc ccagcagaag 2221 gagaagaaag ccactcagat gctcgccatt gttctcggcg tgttcatcat ctgctggctg 2281 cccttcttca tcacacacat cctgaacata cactgtgact gcaacatccc gcctgtcctg 2341 tacagcgcct tcacgtggct gggctatgtc aacagcgccg tgaaccccat catctacacc 2401 accttcaaca ttgagttccg caaggccttc ctgaagatcc tccactgctg agaattcgat 2461 atcaagctta tcgatcatca acctctggat tacaaaattt gtgaaagatt gactggtatt 2521 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat 2581 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct 2641 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct 2701 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc 2761 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg 2821 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc 2881 tttccttggc tgctcgccta tgttgccacc tggattctgc gcgggacgtc cttctgctac 2941 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg 3001 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc 3061 ccgcatcgat accgagcgct gctcgagaga tctacgggtg gcatccctgt gacccctccc 3121 cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt gtcctaataa 3181 aattaagttg catcattttg tctgactagg tgtccttcta taatattatg gggtggaggg 3241 gggtggtatg gagcaagggg caagttggga agacaacctg tagggcctgc ggggtctatt 3301 gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc gcctcctggg 3361 ttcaagcgat tctcctgcct cagcctcccg agttgttggg attccaggca tgcatgacca 3421 ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg gccaggctgg 3481 tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg ctgggattac 3541 aggcgtgaac cactgctccc ttccctgtcc ttctgatttt gtaggtaacc acgtgcggac 3601 cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc 3661 gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc 3721 agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac 3781 gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc 3841 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc 3901 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt 3961 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac 4021 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag 4081 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa 4141 actggaacaa cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg 4201 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac 4261 aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca 4321 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg 4381 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg 4441 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta 4501 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat 4561 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 4621 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa 4681 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac 4741 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac 4801 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt 4861 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc 4921 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca 4981 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc 5041 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag 5101 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa 5161 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg 5221 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa 5281 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg 5341 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt 5401 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt 5461 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag 5521 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat 5581 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 5641 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 5701 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 5761 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 5821 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc 5881 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 5941 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 6001 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 6061 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg 6121 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 6181 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 6241 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 6301 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt //