Basic Vector Information
pLVX-PAmCherry-C1 Vector is an HIV-1-based, lentiviral expression vector. Lentiviral particles derived from the vector allow you to infect cells and express your gene of interest fused to PAmCherry, a photoactivatable mutant of the fluorescent protein mCherry. PAmCherry is non-fluorescence until photoactivated by a short exposure to light at a wavelength between 350 nm and 400 nm. The excitation/emission wavelengths of photoactivated PAmCherry are 564 nm and 595 nm. Genes cloned into the MCS will be expressed as fusions to the C-terminus of PAmCherry if they are in the same reading frame as PAmCherry and there are no intervening stop codons.
- Vector Name:
- pLVX-PAmCherry-C1
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8770 bp
- Type:
- Viral Expression & Packaging Vectors
- Replication origin:
- ori
- Source/Author:
- Clontech
- Selection Marker:
- Puromycin
- Copy Number:
- High copy number
- Promoter:
- mPGK
- Cloning Method:
- Enzyme Cut
- Fusion Tag:
- PAmCherry
- Expression Method:
- Constiutive, Stable
pLVX-PAmCherry-C1 vector Vector Map
pLVX-PAmCherry-C1 vector Sequence
LOCUS V012717 8770 bp DNA circular SYN 08-APR-2021
DEFINITION Exported.
ACCESSION V012717
VERSION V012717
KEYWORDS pLVX-PAmCherry-C1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 8770)
TITLE Direct Submission
REFERENCE 2 (bases 1 to 8770)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..8770
/mol_type="other DNA"
/organism="synthetic DNA construct"
LTR 1..634
/label="3' LTR"
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label="HIV-1 Psi"
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label="RRE"
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1721..1765
/label="gp41 peptide"
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
CDS 1914..1955
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
/label="Protein Tat"
misc_feature 2027..2144
/label="cPPT/CTS"
/note="central polypurine tract and central termination
sequence of HIV-1"
enhancer 2201..2504
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 2505..2708
/label="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 2806..3513
/label="PAmCherry1"
/note="photoactivatable monomeric derivative of DsRed
fluorescent protein (Subach et al., 2009)"
misc_feature 3517..3582
/label="MCS"
/note="multiple cloning site"
promoter 3605..4104
/label="PGK promoter"
/note="mouse phosphoglycerate kinase 1 promoter"
CDS 4125..4721
/label="PuroR"
/note="puromycin N-acetyltransferase"
misc_feature 4738..5326
/label="WPRE"
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
LTR 5533..6166
/label="5' LTR"
/note="5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(6295..6311)
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(6319..6335)
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6343..6373)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(6388..6409)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(6697..7285)
/direction=LEFT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(7459..8316)
/label="AmpR"
/note="beta-lactamase"
promoter complement(8317..8421)
/label="AmpR promoter"
polyA_signal 8469..8603
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
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