LOCUS       V012717                 8770 bp    DNA     circular SYN 08-APR-2021
DEFINITION  Exported.
ACCESSION   V012717
VERSION     V012717
KEYWORDS    pLVX-PAmCherry-C1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8770)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8770)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8770
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        2201..2504
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2806..3513
                     /label="PAmCherry1"
                     /note="photoactivatable monomeric derivative of DsRed
                     fluorescent protein (Subach et al., 2009)"
     misc_feature    3517..3582
                     /label="MCS"
                     /note="multiple cloning site"
     promoter        3605..4104
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             4125..4721
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    4738..5326
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             5533..6166
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6295..6311)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6319..6335)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6343..6373)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6388..6409)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6697..7285)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7459..8316)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8317..8421)
                     /label="AmpR promoter"
     polyA_signal    8469..8603
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tagctagagg atcgctagcg ccaccatggt gagcaagggc
     2821 gaggaggata acatggccat cattaaggag ttcatgcgct tcaaggtgca catggagggg
     2881 tccgtgaacg gccacgtgtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     2941 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcac ctgggacatc
     3001 ctgtcccctc aattcatgta cggctccaat gcctacgtga agcaccccgc cgacatcccc
     3061 gactacttta agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaaattcgag
     3121 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggtga gttcatctac
     3181 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
     3241 atgggctggg aggccctctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     3301 gtcaagccga gagtgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     3361 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaaccg caagttggac
     3421 atcacctcac acaacgagga ctacaccatc gtggaacagt acgaacgtgc cgagggccgc
     3481 cactccaccg gcggcatgga cgagctgtac aaggctaccg gactcagatc tcgagctcaa
     3541 gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat cccgcgactc tagataattc
     3601 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
     3661 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
     3721 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
     3781 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
     3841 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
     3901 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
     3961 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg
     4021 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
     4081 tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc gagtacaagc
     4141 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     4201 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     4261 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     4321 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     4381 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     4441 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     4501 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     4561 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     4621 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     4681 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaccgcgt ctggaacaat
     4741 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     4801 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     4861 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     4921 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     4981 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     5041 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     5101 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     5161 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     5221 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     5281 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattaattct
     5341 gcagtcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg
     5401 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc
     5461 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     5521 aaaagagggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt
     5581 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg
     5641 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg
     5701 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga
     5761 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc
     5821 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca
     5881 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc
     5941 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta
     6001 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     6061 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     6121 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     6181 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggcct
     6241 tgacattgct agcgttttac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     6301 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     6361 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     6421 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     6481 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     6541 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     6601 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     6661 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     6721 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     6781 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     6841 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     6901 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     6961 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7021 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7081 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     7141 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7201 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     7261 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     7321 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     7381 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     7441 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     7501 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     7561 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     7621 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     7681 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     7741 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     7801 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     7861 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     7921 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     7981 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     8041 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     8101 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     8161 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     8221 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     8281 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     8341 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     8401 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
     8461 gggagatcaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     8521 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     8581 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac
     8641 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct
     8701 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
     8761 acttagtagt
//
