pSPL3 vector (V001167)

Price Information

Cat No.	Plasmid Name	Availability	Add to cart
V001167	pSPL3	In stock, instant shipping	Buy one, get one free!

Two tubes of lyophilized plasmid will be delivered, each tube is about 5µg.

Basic Vector Information

It is designed to search genomic DNA for potential exon sequences.

Vector Name:: pSPL3

Antibiotic Resistance:: Ampicillin

Length:: 6031 bp

Type:: Cloning vectors

Replication origin:: ori

pSPL3 vector Vector Map

View Map in NovoBuilder

Plasmid Protocol

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5. Store the plasmid at -20 ℃.

6. The concentration of plasmid re-measurement sometimes differs from the nominal value, which may be due to the position of the lyophilized plasmid in the tube, the efficiency of the re-dissolution, the measurement bias, and adsorption on the wall of the tube, therefore, it is recommended to transform and extract the plasmid before using it

General Plasmid Transform Protocol

1. Take one 100μl of the competent cells and thaw it on ice for 10min, add 2μl of plasmid, then ice bath for 30min, then heat-shock it at 42℃ for 60s, do not stir, and then ice bath for 2min.

2. Add 900μl of LB liquid medium without antibiotics, and incubate at 37℃ for 45min (30℃ for 1-1.5 hours) with 180rpm shaking.

3. Centrifuge at 6000rpm for 5min, leave only 100μl of supernatant to resuspend the bacterial precipitate and spread it onto the target plasmid-resistant LB plate.

4. Invert the plate and incubate at 37℃ for 14h, or at 30℃ for 20h.

5. Pick a single colony into LB liquid medium, add the corresponding antibiotics, incubate at 220rpm for 14h, and extract the plasmid according to the experimental needs and the instructions of the plasmid extraction kit.

pSPL3 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       V001167                 6031 bp    DNA     circular SYN 13-JUL-2022
DEFINITION  Exported.
ACCESSION   V001167
VERSION     V001167
KEYWORDS    pSPL3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6031)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6031)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6031
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..339
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             715..957
                     /gene="vpu"
                     /label="Protein Vpu"
                     /note="Protein Vpu from Human immunodeficiency virus type 1
                     group M subtype B (isolate BH10). Accession#: P69699"
     misc_feature    2485..2718
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     polyA_signal    complement(3263..3397)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        4071..4175
                     /label="AmpR promoter"
     CDS             4176..5033
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5207..5795
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"