LOCUS       V001167                 6031 bp    DNA     circular SYN 13-JUL-2022
DEFINITION  Exported.
ACCESSION   V001167
VERSION     V001167
KEYWORDS    pSPL3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6031)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6031)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6031
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..339
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             715..957
                     /gene="vpu"
                     /label="Protein Vpu"
                     /note="Protein Vpu from Human immunodeficiency virus type 1
                     group M subtype B (isolate BH10). Accession#: P69699"
     misc_feature    2485..2718
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     polyA_signal    complement(3263..3397)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        4071..4175
                     /label="AmpR promoter"
     CDS             4176..5033
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5207..5795
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
       61 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
      121 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
      181 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
      241 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
      301 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggact gtgtttactt
      361 gcaatccccc aaaacagaca gaatggtgca tctgtccagt gaggagaagt ctgcggtcac
      421 tgccctgtgg ggcaaggtga atgtggaaga agttggtggt gaggccctgg gcaggctgct
      481 ggttgtctac ccatggaccc agaggttctt cgagtccttt ggggacctgt cctctgcaaa
      541 tgctgttatg aacaatccta aggtgaaggc tcatggcaag aaggtgctgg ctgccttcag
      601 tgagggtctg agtcacctgg acaacctcaa aggcaccttt gctaagctga gtgaactgca
      661 ctgtgacaag ctgcacgtgc tctagagtcg acccagcagt aagtaataca tgtaatgcaa
      721 cctatacaaa tagcaatagt agcattagta gtagcaataa taatagcaat agttgtgtgg
      781 tccatagtaa tcatagaata taggaaaata ttaagacaaa gaaaaataga caggttaatt
      841 gatagactaa tagaaagagc agaagacagt ggcaatgaga gtgaaggaga aatatcagca
      901 cttgtggaga tgggggtgga gatggggcac catgctcctt gggatgttga tgatctgtag
      961 tgctacagaa aaattgtggg tcacagtcta ttatggggta cgggatcacc agaattctgg
     1021 agctcgagcg gccgctgcag gatcccagat atctggtgat cccgtacctg tgtggaagga
     1081 agcaaccacc actctatttt gtgcatcaga tgctaaagca tatgatacag aggtacataa
     1141 tgtttgggcc acacatgccg gtgtacccac agaccccaac ccacaagaag tagtattggt
     1201 aaatgtgaca gaaaatttta acatgtggaa aaatgacatg gtagaacaga tgcatgagga
     1261 tataatcagt ttatgggatc aaagcctaaa gccatgtgta aaattaaccc cactctgtgt
     1321 tagtttaaag tgcactgatt tgaagaatga tactaatacc aatagtagta gcgggagaat
     1381 gataatggag aaaggagaga taaaaaactg ctctttcaat atcagcacaa gcataagagg
     1441 taaggtgcag aaagaatatg cattttttta taaacttgat ataataccaa tagataatga
     1501 tactaccagc tatacgttga caagttgtaa cacctcagtc attacacagg cctgtccaaa
     1561 ggtatccttt gagccaattc ccatacatta ttgtgccccg gctggttttg cgattctaaa
     1621 atgtaataat aagacgttca atggaacagg accatgtaca aatgtcagca cagtacaatg
     1681 tacacatgga attaggccag tagtatcaac tcaactgctg ttaaatggca gtctagcaga
     1741 agaagaggta gtaattagat ctgtcaattt cacggacaat gctaaaacca taatagtaca
     1801 gctgaacaca tctgtagaaa ttaattgtac aagacccaac aacaatacaa gaaaaaaaat
     1861 ccgtatccag aggggaccag ggagagcatt tgttacaata ggaaaaatag gaaatatgag
     1921 acaagcacat tgtaacatta gtagagcaaa atggaatgcc actttaaaac agatagctag
     1981 caaattaaga gaacaatttg gaaataataa aacaataatc tttaagcaat cctcaggagg
     2041 ggacccagaa attgtaacgc acagttttaa ttgtggaggg gaatttttct actgtaattc
     2101 aacacaactg tttaatagta cttggtttaa tagtacttgg agtactgaag ggtcaaataa
     2161 cactgaagga agtgacacaa tcacactccc atgcagaata aaacaattta taaacatgtg
     2221 gcaggaagta ggaaaagcaa tgtatgcccc tcccatcagc ggacaaatta gatgttcatc
     2281 aaatattaca gggctgctat taacaagaga tggtggtaat aacaacaatg ggtccgagat
     2341 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     2401 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     2461 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     2521 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     2581 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     2641 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     2701 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactacttt gcaccactgc
     2761 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     2821 gtggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     2881 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     2941 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     3001 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttctg tagtgaatag
     3061 agttaggcag ggatattcac cattatcgtt tcagacctgg agatctcccg aggggacccg
     3121 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccatttcga
     3181 ccaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc
     3241 cctggctcac aaataccact gagatccaga catgataaga tacattgatg agtttggaca
     3301 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     3361 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     3421 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     3481 atgtggtatg gctgattatg atccccagga agctcctctg tgtcctcata aaccctaacc
     3541 tcctctactt gagaggacat tccaatcata ggctgcccat ccaccctctg tgtcctcctg
     3601 ttaattaggt cacttaacaa aaaggaaatt gggtaggggt ttttcacaga ccgctttcta
     3661 agggtaattt taaaatatct gggaagtccc ttccactgct gtgttccaga agtgttggta
     3721 aacagcccac aaatgtcaac agcagaaaca tacaagctgt cagctttgca caagggccca
     3781 acaccctgct catcaagaag cactgtggtt gctgtgttag taatgtgcaa aacaggaggc
     3841 acattttccc cacctgtgta ggttccaaaa tatctagtgt tttcattttt acttggatca
     3901 ggaacccagc actccactgg ataagcatta tccttatcca aaacagcctt gtggtcagtg
     3961 ttcatctgct gactgtcaac tgtagcattt tttggggtta cagtttgagc aggatatttg
     4021 gtcctgtagt ttgctaacac accccaggtg gcacttttcg gggaaatgtg cgcggaaccc
     4081 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     4141 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     4201 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     4261 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     4321 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     4381 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     4441 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     4501 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     4561 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     4621 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     4681 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     4741 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     4801 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     4861 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     4921 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     4981 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     5041 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     5101 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatccttaac ggtgagtttt
     5161 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     5221 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5281 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     5341 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     5401 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     5461 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     5521 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     5581 gatacctaca gcgcgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     5641 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     5701 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     5761 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     5821 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     5881 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     5941 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     6001 tccccgcgcg ttggccgatt cattaatgca g
//
