Basic Vector Information
pUCP20, a shuttle vector that replicates in Escherichia coli as well as in Pseudomonas.
- Vector Name:
- pUCP20
- Antibiotic Resistance:
- Ampicillin
- Length:
- 3898 bp
- Copy Number:
- High copy number
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pUCP20 vector Map
References
- Olsen RH, DeBusscher G, McCombie WR. Development of broad-host-range vectors and gene banks: self-cloning of the Pseudomonas aeruginosa PAO chromosome. J Bacteriol. 1982 Apr;150(1):60-9. doi: 10.1128/jb.150.1.60-69.1982. PMID: 6277872; PMCID: PMC220082.
pUCP20 vector Sequence
LOCUS Exported 3898 bp DNA circular SYN 26-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3898)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..3898
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin 232..583
/label=pRO1600 oriV
/note="broad-host-range origin of replication from
Pseudomonas aeruginosa plasmid pRO1600; requires the
pRO1600 Rep protein for replication (West et al., 1994)"
CDS 597..1430
/codon_start=1
/product="replication protein for the broad-host-range
plasmid pRO1600 from Pseudomonas aeruginosa "
/label=pRO1600 Rep
/translation="MASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
primer_bind 1591..1607
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 1609..1665
/label=Multiple Cloning Site (MCS)
/note="A region containing multiple restriction enzyme
recognition sites used for inserting foreign DNA fragments,
commonly derived from the lacZ gene in pUC18 plasmid
vectors for blue-white screening selection.; id: 4341885"
promoter 1682..1804
/label=lac operon promoter
/note="Promoter region that regulates transcription of the
lactose operon genes, including lacZ, controlling bacterial
metabolism of lactose in response to environmental
conditions; id: 3799053"
rep_origin 1980..2743
/label=ColE1 replication origin
/note="Origin of replication from the ColE1 plasmid,
responsible for initiating DNA replication in bacterial
systems; id: 4018607"
CDS complement(2838..3698)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter 3699..3829
/label=AmpR promoter
/note="Regulatory region that initiates transcription of
the ampicillin resistance gene in bacterial plasmids; id:
3672594"
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