LOCUS       Exported                3898 bp DNA     circular SYN 26-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 26, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3898
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      232..583
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from 
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     CDS             597..1430
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pRO1600 from Pseudomonas aeruginosa "
                     /label=pRO1600 Rep
                     /translation="MASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     primer_bind     1591..1607
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1609..1665
                     /label=Multiple Cloning Site (MCS)
                     /note="A region containing multiple restriction enzyme 
                     recognition sites used for inserting foreign DNA fragments,
                     commonly derived from the lacZ gene in pUC18 plasmid 
                     vectors for blue-white screening selection.; id: 4341885"
     promoter        1682..1804
                     /label=lac operon promoter
                     /note="Promoter region that regulates transcription of the 
                     lactose operon genes, including lacZ, controlling bacterial
                     metabolism of lactose in response to environmental 
                     conditions; id: 3799053"
     rep_origin      1980..2743
                     /label=ColE1 replication origin
                     /note="Origin of replication from the ColE1 plasmid,
                     responsible for initiating DNA replication in bacterial 
                     systems; id: 4018607"
     CDS             complement(2838..3698)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        3699..3829
                     /label=AmpR promoter
                     /note="Regulatory region that initiates transcription of
                     the ampicillin resistance gene in bacterial plasmids; id: 
                     3672594"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatacctc tcaggcgccg ctggtgccgc tggttggacg ccaagggtga atccgcctcg
      241 ataccctgat tactcgcttc ctgcgccctc tcaggcggcg ataggggact ggtaaaacgg
      301 ggattgccca gacgcctccc ccgccccttc aggggcacaa atgcggcccc aacggggcca
      361 cgtagtggtg cgttttttgc gtttccaccc ttttcttcct tttccctttt aaacctttta
      421 ggacgtctac aggccacgta atccgtggcc tgtagagttt aaaaagggac ggatttgttg
      481 ccattaaggg acggatttgt tgttaagaag ggacggattt gttgttgtaa agggacggat
      541 ttgttgtatt gtgggacgca gatacagtgt ccccttatac acaaggaatg tcgaacgtgg
      601 cctcaccccc aatggtttac aaaagcaatg ccctggtcga ggccgcgtat cgcctcagtg
      661 ttcaggaaca gcggatcgtt ctggcctgta ttagccaggt gaagaggagc gagcctgtca
      721 ccgatgaagt gatgtattca gtgacggcgg aggacatagc gacgatggcg ggtgtcccta
      781 tcgaatcttc ctacaaccag ctcaaagaag cggccctgcg cctgaaacgg cgggaagtcc
      841 ggttaaccca agagcccaat ggcaagggga aaagaccgag tgtgatgatt accggctggg
      901 tgcaaacaat catctaccgg gagggtgagg gccgtgtaga actcaggttc accaaagaca
      961 tgctgccgta cctgacggaa ctcaccaaac agttcaccaa atacgccttg gctgacgtgg
     1021 ccaagatgga cagcacccac gcgatcaggc tttacgagct gctcatgcaa tgggacagca
     1081 tcggccagcg cgaaatagaa attgaccagc tgcgaaagtg gtttcaactg gaaggccggt
     1141 atccctcgat caaggacttc aagttgcgag tgcttgatcc agccgtgacg cagatcaacg
     1201 agcacagccc gctacaggtg gagtgggcgc agcgaaagac cgggcgcaag gtcacacatc
     1261 tgttgttcag ttttggaccg aagaagcccg ccaaggcggt gggtaaggcc ccagcgaagc
     1321 gcaaggccgg gaagatttca gatgctgaga tcgcgaaaca ggctcgccct ggtgagacat
     1381 gggaagcggc ccgcgctcga ctaacccaga tgccgctgga tctggcctag aggccgtggc
     1441 caccacggcc cggcctgcct ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     1501 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     1561 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagcttgcat
     1621 gcctgcaggt cgactctaga ggatccccgg gtaccgagct cgaattcgta atcatggtca
     1681 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     1741 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     1801 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     1861 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     1921 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     1981 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     2041 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     2101 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     2161 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     2221 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     2281 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     2341 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     2401 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     2461 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     2521 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     2581 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     2641 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     2701 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     2761 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     2821 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     2881 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     2941 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     3001 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     3061 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     3121 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     3181 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     3241 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     3301 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     3361 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     3421 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     3481 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     3541 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     3601 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     3661 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     3721 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     3781 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     3841 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtc
//
